Reactivation of antibiosis in the entomogenous fungus Chrysoporthe sp. SNB-CN74

Nirma, Charlotte; Éparvier, Véronique; Stien, Didier

doi:10.1038/ja.2015.36

Cited by 16 publications

(17 citation statements)

References 32 publications

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“…Insect–microorganism interactions have been widely studied within apocrites (bees, wasps, ants), but few studies are related to termite–microorganism interactions outside the trophobiosis [ 5 , 6 , 7 , 8 ]. However, examples of antimicrobial compounds produced by microorganisms associated with termites from French Guiana were previously published in the literature, especially by the research group of D. Stien and V. Eparvier [ 9 , 10 , 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Dereplication, Annotation, and Characterization of 74 Potential Antimicrobial Metabolites from Penicillium Sclerotiorum Using t-SNE Molecular Networks

et al. 2021

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Microorganisms associated with termites are an original resource for identifying new chemical scaffolds or active metabolites. A molecular network was generated from a collection of strain extracts analyzed by liquid chromatography coupled to tandem high-resolution mass spectrometry, a molecular network was generated, and activities against the human pathogens methicillin-resistant Staphylococcus aureus, Candida albicans and Trichophyton rubrum were mapped, leading to the selection of a single active extract of Penicillium sclerotiorum SNB-CN111. This fungal species is known to produce azaphilones, a colorful family of polyketides with a wide range of biological activities and economic interests in the food industry. By exploring the molecular network data, it was shown that the chemical diversity related to the P. sclerotiorum metabolome largely exceeded the data already reported in the literature. According to the described fragmentation pathways of protonated azaphilones, the annotation of 74 azaphilones was proposed, including 49 never isolated or synthesized thus far. Our hypothesis was validated by the isolation and characterization of eight azaphilones, among which three new azaphilones were chlorogeumasnol (63), peniazaphilone E (74) and 7-deacetylisochromophilone VI (80).

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Section: Introductionmentioning

confidence: 99%

Dereplication, Annotation, and Characterization of 74 Potential Antimicrobial Metabolites from Penicillium Sclerotiorum Using t-SNE Molecular Networks

et al. 2021

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“…[20][21][22] Small changes in culture conditions or cocultivation with other microbial species may activate cryptic biosynthetic pathways. [23][24][25] Therefore, to analyze substantial subsequent production of the metabolites identified, the fungal strain was cultivated on seven different culture media.…”

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confidence: 99%

Paecilosetin Derivatives as Potent Antimicrobial Agents from Isaria farinosa

et al. 2020

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A screening of 57 entomopathogenic microorganisms against human pathogens was subjected to mass spectrometry molecular networking-based dereplication. A particular strain of fungus, Isaria farinosa BSNB-1250, producing potentially both active and novel metabolites, was grown on a large-scale on potato dextrose agar (PDA) medium. Paecilosetin (1) and five new analogues (2-6) were isolated. The structures of the new compounds were elucidated using 1D and 2D NMR. The absolute configurations of compounds 1-6 were determined by Mosher ester derivatives (1), comparison of experimental and calculated ECD spectra (2-4 and 6), and singlecrystal X-ray diffraction analysis (5). Compounds 1-6 were assayed against methicillin-sensitive (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA), Escherichia coli, Candida albicans, Trichophyton rubrum and the MRC-5 cell line. Compounds 1 and 5 exhibited strong antibacterial activity against MSSA and MRSA with MIC values of 1-2 µg/mL.

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“…Chrysoporthe sp. exhibited strong antibacterial activity against E. coli and S. aureus (Nirma et al, 2015); and Fusarium species produced metabolites with activities against microbial pathogens (Sibero et al, 2019). Besides, from F. larvarum, an antifungal compound active against C. albicans and a wide range of pathogenic fungi was isolated, called Parafungin (Harvey et al, 2015).…”

Section: Fungal Strain Code Labmicramentioning

confidence: 99%

Antimicrobial and cytotoxic activity of fungal mycelial extracts from aquatic environments in the Amazon

Oliveira

Santiago

Katak

et al. 2021

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Fungi are a prolific source of biologically active metabolites, including a wide range of clinically important drugs. Therefore, this study aims to evaluate the antimicrobial and cytotoxic activity of secondary metabolites extracted from fungal mycelia isolated from freshwater samples in the state of Amazonas. Mycelial extracts from 12 fungal were used, extracted with MeOH/AcOEt (1:1) according to the criteria established by Souza et al. (2004). For antimicrobial activity, the extracts were tested against the pathogens Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Enterococcus feacalis, Candida albicans and C. tropicalis. To identify the minimum inhibitory dosage (MID) the microdilution method was used. To perform the cytotoxicity assay, the VERO strain (ATCC® CCL-81TM) was used. The assays were determined by the Alamar Blue method according to Ahmed et al. (1994). The tested extracts did not show antibacterial activity. Five extracts (41.7%), obtained from the fungi Aspergillus - 1283, Chrysoporther - 1169, Diaporther – 1203, Fusarium – 1085, and Trichoderma, showed antifungal activity against C. albicans. Diaporther extract (8.3%) - 1203 was active against C. tropicalis. In the cytotoxicity assay, 58.3% of the evaluated extracts showed no significant toxic effect. Five extracts, Cladosporium - 1135, Chrysoporther - 1169, Cytospora - 1098, Fusarium - 1085, and Talaromyces - 1244, showed cytotoxic potential, exhibiting viability lower than 70%. The results obtained suggest that mycelial extracts of fungi isolated from water samples from the Amazon region have potential against yeasts of medical interest. Only two of the active extracts were revealed potentially cytotoxic.

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Reactivation of antibiosis in the entomogenous fungus Chrysoporthe sp. SNB-CN74

Cited by 16 publications

References 32 publications

Dereplication, Annotation, and Characterization of 74 Potential Antimicrobial Metabolites from Penicillium Sclerotiorum Using t-SNE Molecular Networks

Dereplication, Annotation, and Characterization of 74 Potential Antimicrobial Metabolites from Penicillium Sclerotiorum Using t-SNE Molecular Networks

Paecilosetin Derivatives as Potent Antimicrobial Agents from Isaria farinosa

Antimicrobial and cytotoxic activity of fungal mycelial extracts from aquatic environments in the Amazon

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