Reactions of 2,2-dimethylaziridine-type Alkylating Agents in Biological Systems I: Colorimetric Estimation and Stability in Physiological Media

Lalka, David; Bardos, Thomas J.

doi:10.1002/jps.2600620813

Cited by 7 publications

(2 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Various well-developed methods, including non-aqueous titration (Ciaccio et al, 1957), gravimetry (Nnaji et al, 2017), colorimetry (Lalka et al, 2010), spectrophotometry (Hayat et al, 2017), liquid chromatography (Park et al, 2016), liquid chromatography-tandem mass spectrometry (Lin et al, 2010;Xie et al, 2016) and gas chromatography-tandem mass spectrometry (Wang et al, 2017), have been reported for the measurement of piperazine and its salt residues. Nevertheless, traditional methods face many operational challenges; the processes are often quite cumbersome and time-consuming, or the detection is susceptible to substantial interference from impurities.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The establishment of a practical method for the determination of piperazine residues using accelerated solvent extraction and UHPLC-FLD

Guo

Xie

Wang

et al. 2020

Qual. Assur. Saf. Crops Foods

View full text Add to dashboard Cite

This article describes a reliable method for estimating piperazine residues in chicken tissues (muscle, kidney and liver) and pork via ultrahigh-performance liquid chromatography (UHPLC) coupled with a fluorescence detector (FLD) using dansyl chloride (DNS-Cl) as the derivatisation reagent. After extraction by accelerated solvent extraction (ASE), the analyte was purified on a strong cation-exchange solid-phase extraction (SPE) column. Separation was achieved using an Acquity UPLC HSS T3 (2.1 mm × 100 mm, 1.8 μm) column with ultrapure water-acetonitrile (15:85, V/V) as the mobile phase. The results showed that when the concentration of piperazine was between the limit of quantitation (LOQ) and 200.0 μg/kg, the peak area of the derivative had a good linear relationship with the piperazine concentration, and the coefficients of determination (R 2 ) were greater than or equal to 0.9991. When the spiked concentration of piperazine was equal to the LOQ, maximum residue limit (MRL) of 0.5, 1.0 and 2.0, the recoveries ranged from 79.64 to 99.77% and the relative standard deviations (RSDs) were 1.14-5.63%. The limits of detection (LODs) and LOQs were 0.50-1.20 and 1.80-3.50 μg/kg, respectively. The method was applied to the quantification of piperazine residues in commercial chicken tissues and pork from local supermarkets.

show abstract

Section: Introductionmentioning

confidence: 99%

“…Gravimetry is cumbersome and has a large measurement error for low-abundance components (Nnaji et al, 2017). The applicable range of colorimetry is limited (Lalka et al, 2010). Spectrophotometry is affected by the light source, pH, cuvette material and other factors.…”

Section: Introductionmentioning

confidence: 99%