Reaction of the Mouse Uterus During Implantation and Deciduoma Formation as Demonstrated by Changes in the Distribution of Alkaline Phosphatase

Finn, C. A.; Hinchliffe, J. R.

doi:10.1530/jrf.0.0080331

Cited by 130 publications

(98 citation statements)

References 12 publications

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“…These are characteristic features of the decidual reaction and have been described previously (Finn and Hinchliffe 1964;Murdoch et at. 1978).…”

Section: Discussionsupporting

confidence: 72%

“…In this context, alkaline phosphatase (orthophosphoric monoester phosphohydrolase, EC 3.1.3.1) from the pregnant mouse uterus is a zinc-containing metalloenzyme and is also inhibited in vitro by cadmium (Murdoch et al 1980). This enzyme is associated with the induction of the decidual cell reaction during early pregnancy (Finn and Hinchliffe 1964;Murdoch et al 1978) and may play an important role in facilitating maternal-embryonic relationships during the peri-implantation period. It is possible, therefore, that in vivo inhibition of alkaline phosphatase in uterine decidual cells by cadmium could lead to functional alterations in the decidua which may be detrimental to the developing embryo.…”

Section: Introductionmentioning

confidence: 99%

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Foetotoxicity of Cadmium in Quackenbush Strain Mice and the Effects of the Metal on Uterine Alkaline Phosphatase During Pseudopregnancy

Murdoch¹,

Cowen²

1981

Aust. Jnl. Of Bio. Sci.

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Experiments were conducted to determine the sensitivity of Quackenbush strain (QS) mice to the foetotoxic effects of single subcutaneous injections of CdClz (2, 4 and 8 mg of cadmium per kilogram body weight) on days 1, 2, 4 or 8 of pregnancy. Autopsies performed on day 16 of pregnancy revealed that cadmium was teratogenic and increased the incidence of malformed forelimbs and exencephaly and decreased foetal weight. The metal caused foetal weight changes and exencephaly only when it was administered on day 8 of pregnancy indicating that the post-implantation stage presents the QS mouse with a period of increased sensitivity to the foetotoxic effects of cadmium.Although injections of cadmium (8 mg per kilogram body weight) during early pseudopregnancy did not prevent the uterus from undergoing a decidual reaction in response to a peanut oil stimulus, they did in some cases prevent alkaline phosphatase from reaching maximal activity levels in decidualized uterine horns. Thus, inhibition of alkaline phosphatase occurred only in response to the metal injected on day 4 of pseudopregnancy and the enzyme was not affected when cadmium was administered on days 1,2, 3, 5 or 6 of pseudopregnancy.The results suggested that changes in either progesterone and oestrogen production by the ovary or the alkaline phosphatase activity of the uterus are unlikely to be responsible to any great extent for the teratogenic effects of single subcutaneous injections of cadmium in the QS mouse during early pregnancy.

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“…These are characteristic features of the decidual reaction and have been described previously (Finn and Hinchliffe 1964;Murdoch et at. 1978).…”

Section: Discussionsupporting

confidence: 72%

Section: Introductionmentioning

confidence: 99%

Foetotoxicity of Cadmium in Quackenbush Strain Mice and the Effects of the Metal on Uterine Alkaline Phosphatase During Pseudopregnancy

Murdoch¹,

Cowen²

1981

Aust. Jnl. Of Bio. Sci.

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“…Further, the decidual cells in the LIF-replaced glandless Pgr Cre/+ Foxa2 f/f mice on GD 5.5 were clearly abnormal based on decidual marker gene analysis; Alpl, Bmp2, Bmp8a, Prl8a2, and Wnt4 were substantially lower in the implantation sites in these mice than in control and LIF-replaced Ltf iCre/+ Foxa2 f/f mice. BMP2 and WNT4 are key regulators of stromal cell decidualization (34,57), and ALPL, BMP8A, and PRL8A2 are expressed only by fully differentiated decidual cells (57)(58)(59). Of note, complete embryo resorption occurred by GD 9.5 in LIF-replaced glandless Pgr Cre Foxa2 f/f mice, likely because pf inadequate formation of the decidua (7).…”

Section: On Gd 35 Induces the Expression Of Lif In The Glands Of Thementioning

confidence: 99%

Forkhead box a2 (FOXA2) is essential for uterine function and fertility

Kelleher

Wang

Pru

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

122

170

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Establishment of pregnancy is a critical event, and failure of embryo implantation and stromal decidualization in the uterus contribute to significant numbers of pregnancy losses in women. Glands of the uterus are essential for establishment of pregnancy in mice and likely in humans. Forkhead box a2 (FOXA2) is a transcription factor expressed specifically in the glands of the uterus and is a critical regulator of postnatal uterine gland differentiation in mice. In this study, we conditionally deleted FOXA2 in the adult mouse uterus using the lactotransferrin Cre (Ltf-Cre) model and in the neonatal mouse uterus using the progesterone receptor Cre (Pgr-Cre) model. The uteri of adult FOXA2-deleted mice were morphologically normal and contained glands, whereas the uteri of neonatal FOXA2-deleted mice were completely aglandular. Notably, adult FOXA2-deleted mice are completely infertile because of defects in blastocyst implantation and stromal cell decidualization. Leukemia inhibitory factor (LIF), a critical implantation factor of uterine gland origin, was not expressed during early pregnancy in adult FOXA2-deleted mice. Intriguingly, i.p. injections of LIF initiated blastocyst implantation in the uteri of both gland-containing and glandless adult FOXA2-deleted mice. Although pregnancy was rescued by LIF and was maintained to term in uterine gland-containing adult FOXA2-deleted mice, pregnancy failed by day 10 in neonatal FOXA2-deleted mice lacking uterine glands. These studies reveal a previously unrecognized role for FOXA2 in regulation of adult uterine function and fertility and provide original evidence that uterine glands and, by inference, their secretions play important roles in blastocyst implantation and stromal cell decidualization.T he uterus is comprised of two tissue compartments, the endometrium and the myometrium. The endometrium contains three cell types, luminal epithelium (LE), glandular epithelium (GE), and stroma. In mice, the uterus becomes receptive to blastocyst implantation on gestational day (GD) 4 (with the observation of a postcoital vaginal plug designated GD 0.5); it is prereceptive on GD 1-3, and by the afternoon of GD 5 it becomes nonreceptive (refractory) (1-3). Dynamic changes in ovarian estrogen and progesterone production act via the uterus to regulate uterine receptivity, blastocyst implantation, and stromal cell decidualization necessary for the establishment of pregnancy (4-6). The implantation process, which is initiated by the attachment of the blastocyst trophectoderm to the receptive LE, occurs before or right after midnight in the evening of GD 4 and becomes more prominent by the morning of GD 5. By GD 6, the trophectoderm begins to contact directly stromal cells that then begin to differentiate into decidual cells, which are required for successful pregnancy because they regulate placental development and local maternal immune responses (7,8).The infertility observed in leukemia inhibitory factor (Lif)-null mice and uterine gland-knockout (UGKO) mice and sheep established ...

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“…These foci of decidual induction grow to give a series of discrete swellings along the length of the horn (Finn & Hinchliffe, 1964). It is my experience that the induction of decidualization using intrauterine oil results in a series of unevenly-sized, irregularly-spaced decidual swellings at 2-4 days after induction.…”

Section: Resultsmentioning

confidence: 99%

Decidualization in the post-partum uterus of the mouse

Brandon

1990

Reproduction

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Summary. Unilaterally ovariectomized mice were allowed to complete one pregnancy before the second ovary was removed and sensitivity to decidualization was induced by hormone administration. The virgin uterine horn and the post-partum horn were stimulated to decidualize by the intraluminal injection of arachis oil. A greater decidual response was found in post-partum horns than in virgin horns. The foci of decidual induction in post-partum horns were regularly spaced reflecting the regular spacing of used and unused areas of the uterus. A focus of decidual induction occurred in 68% of the recently used uterine areas observed, i.e. areas associated with post-partum nodules. When compared with foci of decidualization in adjacent unused areas of the uterus, none of the decidualized used zones showed more decidual tissue, about half showed less decidual tissue and half showed the same amount of decidual tissue. The remaining 32% of used zones were not associated with foci of decidual induction. The results indicate that decidualization can be induced in recently used zones of the uterus using a non-traumatic method of induction, but that used zones are associated with less decidual tissue than unused zones of the post-partum uterus.

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Reaction of the Mouse Uterus During Implantation and Deciduoma Formation as Demonstrated by Changes in the Distribution of Alkaline Phosphatase

Cited by 130 publications

References 12 publications

Foetotoxicity of Cadmium in Quackenbush Strain Mice and the Effects of the Metal on Uterine Alkaline Phosphatase During Pseudopregnancy

Foetotoxicity of Cadmium in Quackenbush Strain Mice and the Effects of the Metal on Uterine Alkaline Phosphatase During Pseudopregnancy

Forkhead box a2 (FOXA2) is essential for uterine function and fertility

Decidualization in the post-partum uterus of the mouse

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