Reaction of rat connective tissue to a new calcium hydroxide–based sealer

Gomes‐Filho, João Eduardo; Bernabé, Pedro Felício Estrada; Nery, Mauro Juvenal; Otoboni-Filho, José Arlindo; Dezan, Elói; Costa, Mariana Machado Teixeira de Moraes; Faria, Max Douglas; Watanabe, Simone; Gomes, Alessandra Rodrigues

doi:10.1016/j.tripleo.2008.03.030

Cited by 29 publications

(39 citation statements)

References 23 publications

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“…34 In a calcium release study, Sealapex presented high calcium and hydroxyl release, particularly after longer time intervals, which were verified in this in vivo study by the presence of birefrigent granulations under PL from day 7 to day 60, and biomineralization at all time periods. 36 Previous studies with the original formulation of Sealapex presented mild inflammation during initial periods, which reduced over time.…”

Section: Discussionsupporting

confidence: 76%

Biocompatibility and biomineralization assessment of bioceramic-, epoxy-, and calcium hydroxide-based sealers

et al. 2016

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Obturation of the root canal system aims to fill empty spaces, promoting hermetic sealing and preventing bacterial activity in periapical tissues. This should provide optimal conditions for repair, stimulating the process of biomineralization. An endodontic sealer should be biocompatible once it is in direct contact with periapical tissues. The aim of this study was to evaluate the rat subcutaneous tissue response to implanted polyethylene tubes filled with Smartpaste Bio, Acroseal, and Sealapex and investigate mineralization ability of these endodontic sealers. Forty Wistar rats were assigned to the three sealers groups and control group, (n = 10 animals/group) and received subcutaneous implants containing the test sealers, and the control group were implanted with empty tubes. After days 7, 15, 30, and 60, animals were euthanized and polyethylene tubes were removed with the surrounding tissues. Inflammatory infiltrate and thickness of the fibrous capsule were histologically evaluated. Mineralization was analyzed by Von Kossa staining and polarized light. Data were tabulated and analyzed via Kruskal-Wallis and Dunn's test. All tested materials induced a moderate inflammatory reaction in the initial periods. Smartpaste Bio induced the mildest inflammatory reactions after day 15. No difference was observed among groups after days 30 or 60. Von Kossa-positive staining and birefringent structures observed under polarized light revealed a larger mineralization area in Sealapex-treated animals followed by Smartpaste Bio-treated animals. At the end of the experiment, all tested sealers were found to be biocompatible. All sealers induced biomineralization, except Acroseal, which induced a mild tissue reaction.

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Section: Discussionsupporting

confidence: 76%

Biocompatibility and biomineralization assessment of bioceramic-, epoxy-, and calcium hydroxide-based sealers

et al. 2016

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“…Therefore, an endodontic material must be biocompatible and have the ability to allow or induce bone repair 3) . Implantation experiments in which materials come in direct contact with subcutaneous or osseous tissues have been widely accepted as methods to evaluate their biocompatibility 15,16) . Implantation periods used in this study were consistent with the majority of studies carried out on tissue reactions to materials implanted in subcutaneous connective tissue or bone tissue 14,17) .…”

Section: Discussionmentioning

confidence: 99%

Tissue reactions after subcutaneous and intraosseous implantation of iRoot SP, MTA and AH Plus

Zhang

Peng

2015

Dental Materials Journal

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The purpose of this study was to evaluate the reactions of rat subcutaneous connective tissue and bone tissue to iRoot SP, ProRoot MTA, and AH Plus. Specimens were implanted in the dorsal subcutaneous connective tissue and tibias of 36 Wistar rats. Tissue specimens were collected after rats were sacrificed at 7, 30, and 60 days after implantation. Histologic evaluations comprising inflammation severity and new bone formation were blindly made on hematoxylin and eosin-stained sections. In subcutaneous implant test, AH Plus group showed more infiltration of inflammatory cells than the other three groups at 30 days (p<0.05). In intraosseous implant test, number of inflammatory cells decreased in all groups at 7, 30, and 60 days after implantation with no statistically significant differences. Given the similar inflammatory reactions to iRoot SP and MTA, it was concluded that iRoot SP and MTA were biocompatible with the subcutaneous and intraosseous tissues of rats.

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“…Endodontic treatment aimed to restore the normality of apical and periapical tissues [1][2][3][4] through a root canal system deep cleaning and disinfection, to control pathogenic microorganisms and the complete three-dimensional sealing using root canal sealers [5][6][7]. To achieve this goal, irrigation and aspiration of the root canal system, coupled with instrumentation, composes an important stage of radical endodontic treatment, the chemical mechanical preparation.…”

Section: Introductionmentioning

confidence: 99%

Ethanolic Extract of Psidium cattleianum as Intracanal Irrigation: An In vitro Study of Cell Viability

Martins¹

2017

J. Dent. Sci. Ther

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The aim of this study was to evaluate the ethanolic extract of Psidium cattleianum biocompatibility by structural and functional cell viability maintenance. Cell viability was analyzed after 1 h, 3 h, 6 h, 10 h and 24 h of incubation by Exclusion Method with trypan blue and MTT assay, using human mononuclear cells (PBMC) and human periodontal ligament (PDL) cells in culture. Positive control used was RPMI cell culture medium and negative control was ethanol 80%. The obtained data were subjected to statistical analysis at a significance level of 5%. By trypan blue assay, ethanolic extract presented a homogeneous performance of PBMC from the 1 h, with average viability between 30 and 50% (p>0.05). By MTT assay, the results showed the efficacy of the ethanolic extract of Psidium cattleianum in maintaining the functional viability of the periodontal ligament cells to 10 h (p>0.05), presenting values greater than the RPMI control. In both methods, it can be observed that the extract properties can neutralize the cytotoxic effect of its diluent ethanol 80%, which showed average viability nearly zero (p <0.05). Thus, biocompatibility by structural and functional cell viability maintenance was considered acceptable to ethanolic extract of Psidium cattleianum and further studies should be performed to indicate its use as an alternative to irrigation solution traditionally used in everyday practice.

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Reaction of rat connective tissue to a new calcium hydroxide–based sealer

Cited by 29 publications

References 23 publications

Biocompatibility and biomineralization assessment of bioceramic-, epoxy-, and calcium hydroxide-based sealers

Biocompatibility and biomineralization assessment of bioceramic-, epoxy-, and calcium hydroxide-based sealers

Tissue reactions after subcutaneous and intraosseous implantation of iRoot SP, MTA and AH Plus

Ethanolic Extract of Psidium cattleianum as Intracanal Irrigation: An In vitro Study of Cell Viability

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