Reaction of human macrophages on protein corona covered TiO2 nanoparticles

Borgognoni, Camila Figueiredo; Mormann, Michael; Qu, Ying; Schäfer, Marcus; Langer, Klaus; Öztürk, Cengiz; Wagner, Sylvia; Chen, Chunying; Yang, Zhao; Fuchs, Harald; Riehemann, Kristina

doi:10.1016/j.nano.2014.10.001

Cited by 35 publications

(24 citation statements)

References 23 publications

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“…14-15 For example, proteins can rapidly adsorb onto nanoparticles and form the so-called "proteins corona". [16][17][18][19][20] The formation of proteins corona will cover the original surface properties of nanomaterials and become the real substance our organs and cells firstly see. 17,21 As a result, the in vivo fate of nanomaterials becomes complicated no matter how precisely they are initially designed.…”

Section: Introductionmentioning

confidence: 99%

Insight into the Interaction of Graphene Oxide with Serum Proteins and the Impact of the Degree of Reduction and Concentration

Wei

Hao

Shao

et al. 2015

ACS Appl. Mater. Interfaces

114

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As novel applied nanomaterials, both graphene oxide (GO) and its reduced form (rGO) have attracted global attention, because of their excellent properties. However, the lack of comprehensive understanding of their interactions with biomacromolecules highly limits their biomedical applications. This work aims to initiate a systematic study on the property changes of GO/rGO upon interaction with serum proteins and on how their degree of reduction and exposure concentration affect this interaction, as well as to analyze the possible biomedical impacts of the interaction. We found that the adsorption of proteins on GO/rGO occurred spontaneously and rapidly, leading to significant changes in size, zeta potential, and morphology. Compared to rGO, GO showed a higher ability in quenching intrinsic fluorescence of serum proteins in a concentration-dependent manner. The protein adsorption efficiency and the types of associated proteins varied, depending on the degree of reduction and concentration of graphene. Our findings indicate the importance of evaluating the potential protein adsorption before making use of GO/rGO in drug delivery, because the changed physicochemical properties after protein adsorption will have significant impacts on safety and effectiveness of these delivery systems. On the other hand, this interaction can also be used for the separation, purification, or delivery of certain proteins.

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Section: Introductionmentioning

confidence: 99%

Insight into the Interaction of Graphene Oxide with Serum Proteins and the Impact of the Degree of Reduction and Concentration

Wei

Hao

Shao

et al. 2015

ACS Appl. Mater. Interfaces

114

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“…Our group recently investigated the characteristics of the protein corona of TiO 2 nanoparticles and the interaction of these particles with primary cells of human macrophages. We observed preferentially secondary modified protein in the corona of nanoparticles and IL-6 gene secretion by the macrophages even in low nanoparticle concentrations [26]. Vogt et al [27] have shown that the protein corona modulates cellular uptake of silica-coated superparamagnetic iron oxide nanoparticles (SPIONs) in primary human macrophages.…”

Section: Nanomaterials Recognition By Macrophagesmentioning

confidence: 75%

“…Surface coating, a preset to alter undesirable properties of nanoparticles, can highly affect the nanoparticle-protein corona since the surface is the first contact site of the nanoparticles with the cellular environment. Consequently, the interaction of nanoparticles with cells occurs via this protein corona [26]. Uncoated TiO 2 nanoparticles adsorbed more proteins than alumina and silicone-coated particles in contact with primary human monocyte-derived macrophages (GM-CSF-differentiated) as demonstrated by Sund et al [84].…”

Section: Physicochemical Properties Of Metal-oxide Nanomaterials On Mmentioning

confidence: 95%

Human macrophage responses to metal-oxide nanoparticles: a review

Borgognoni

Kim

Zucolotto

et al. 2018

Artificial Cells, Nanomedicine, and Biotechnology

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Nanomaterials have been widely used in our daily lives in medicine, cosmetics, paints, textiles and food products. Many studies aim to determine their biological effects in different types of cells. The interaction of these materials with the immune system leads to reactions by modifying the susceptibility or resistance of the host body which could induce adverse health effects. Macrophages, as specific cells of the innate immune response, play a crucial role in the human defence system to foreign agents. They can be used as a reliable test object for the investigation of immune responses under nanomaterials exposure displayed by expression of a variety of receptors and active secretion of key signalling substances for these processes. This report covers studies of human macrophage behaviours upon exposure of nanomaterials. We focused on their interaction with metal-oxide nanoparticles as these are largely used in medical and cosmetics applications. The discussion and summary of these studies can guide the development of new nanomaterials, which are, at the same time, safe and useful for new purposes, especially for health applications.ARTICLE HISTORY

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“…This effect is described for macrophages after TiO 2 /protein corona contact, indicating a central role of this cytokine during interaction of specific nanoparticles with cells. 46 The downregulation of IL-6 by the nanoparticles used for these experiments is a clear indication of their biocompatibility.…”

Section: Discussionmentioning

confidence: 99%

Comparison of cellular effects of starch-coated SPIONs and poly(lactic-co-glycolic acid) matrix nanoparticles on human monocytes

Gonnissen¹,

Qu²,

Langer³

et al. 2016

IJN

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Within the last years, progress has been made in the knowledge of the properties of medically used nanoparticles and their toxic effects, but still, little is known about their influence on cellular processes of immune cells. The aim of our comparative study was to present the influence of two different nanoparticle types on subcellular processes of primary monocytes and the leukemic monocyte cell line MM6. We used core-shell starch-coated superparamagnetic iron oxide nanoparticles (SPIONs) and matrix poly(lactic-co-glycolic acid) (PLGA) nanoparticles for our experiments. In addition to typical biocompatibility testing like the detection of necrosis or secretion of interleukins (ILs), we investigated the impact of these nanoparticles on the actin cytoskeleton and the two voltage-gated potassium channels Kv1.3 and Kv7.1. Induction of necrosis was not seen for PLGA nanoparticles and SPIONs in primary monocytes and MM6 cells. Likewise, no alteration in secretion of IL-1β and IL-10 was detected under the same experimental conditions. In contrast, IL-6 secretion was exclusively downregulated in primary monocytes after contact with both nanoparticles. Two-electrode voltage clamp experiments revealed that both nanoparticles reduce currents of the aforementioned potassium channels. The two nanoparticles differed significantly in their impact on the actin cytoskeleton, demonstrated via atomic force microscopy elasticity measurement and phalloidin staining. While SPIONs led to the disruption of the respective cytoskeleton, PLGA did not show any influence in both experimental setups. The difference in the effects on ion channels and the actin cytoskeleton suggests that nanoparticles affect these subcellular components via different pathways. Our data indicate that the alteration of the cytoskeleton and the effect on ion channels are new parameters that describe the influence of nanoparticles on cells. The results are highly relevant for medical application and further evaluation of nanomaterial biosafety.

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Reaction of human macrophages on protein corona covered TiO2 nanoparticles

Cited by 35 publications

References 23 publications

Insight into the Interaction of Graphene Oxide with Serum Proteins and the Impact of the Degree of Reduction and Concentration

Insight into the Interaction of Graphene Oxide with Serum Proteins and the Impact of the Degree of Reduction and Concentration

Human macrophage responses to metal-oxide nanoparticles: a review

Comparison of cellular effects of starch-coated SPIONs and poly(lactic-co-glycolic acid) matrix nanoparticles on human monocytes

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