Reaction of Flax (Linum Usitatissimum L.) Calli Culture to Supplement of Medium by Carbon Nanoparticles

Kokina, Inese; Sļedevskis, Ē.; Gerbreders, V.; Grauda, Dace; Jermaļonoka, Marija; Valaine, Kristīna; Gavarāne, Inese; Pigiņka, Inga; Filipovičs, Maksims; Rashal, Isaak

doi:10.2478/v10046-012-0010-3

Cited by 10 publications

(15 citation statements)

References 65 publications

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“…The mean number of regeneration zones was variable in all groups. Previous study showed that carbon nanoparticles can increase mean of regeneration zones in flax callus [24]. In present study, the mean number of regeneration zones in control group was the lowest ( n = 1.24 ± 0.48), although in experimental groups in calluses grown in medium supplemented by Au ( n = 3.29 ± 1.07) or Ag ( n = 2.01 ± 0.98) it increased.…”

Section: Resultscontrasting

confidence: 46%

Case Study of Somaclonal Variation in Resistance GenesMloandPme3in Flaxseed (Linum usitatissimumL.) Induced by Nanoparticles

Kokina

Mickeviča

Jermaļonoka

et al. 2017

International Journal of Genomics

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Nanoparticles influence on genome is investigated worldwide. The appearance of somaclonal variation is a cause of great concern for any micropropagation system. Somaclonal variation describes the tissue-culture-induced phenotypic and genotypic variations. This paper shows the results of somaclonal variation in two resistance genes pectin methylesterase and Mlo-like protein in all tissue culture development stages, as donor plant, calluses, and regenerants of Linum usitatissimum induced by gold and silver nanoparticles. In this paper, it was essential to obtain DNA material from all tissue culture development stages from one donor plant to record changes in each nucleotide sequence. Gene region specific primers were developed for resistance genes such as Mlo and Pme3 to define the genetic variability in tissue culture of L. usitatissimum. In recent years, utilization of gold and silver nanoparticles in tissue culture is increased and the mechanisms of changes in genome induced by nanoparticles still remain unclear. Obtained data show the somaclonal variation increase in calluses obtained from one donor plant and grown on medium supplemented by gold nanoparticles (Mlo 14.68 ± 0.98; Pme3 2.07 ± 0.87) or silver nanoparticles (Mlo 12.01 ± 0.43; Pme3 10.04 ± 0.46) and decrease in regenerants. Morphological parameters of calluses showed a number of differences between each investigated culture group.

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Section: Resultscontrasting

confidence: 46%

Case Study of Somaclonal Variation in Resistance GenesMloandPme3in Flaxseed (Linum usitatissimumL.) Induced by Nanoparticles

Kokina

Mickeviča

Jermaļonoka

et al. 2017

International Journal of Genomics

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“…64 The presence of carbon NPs in the MS medium reduced the frequency of callus induction in Linum usitatissimum. 65 Syu et al 66 investigated the effects of three different morphologies of Ag NPs, including decahedral (45 AE 5 nm), spherical (8 AE 2 nm) and triangular (47 AE 7 nm), on seedling growth, gene expression and physiological changes in Arabidopsis (Table 1). Root growth was enhanced when the seedlings were treated with either triangular or decahedral Ag NPs, whereas it was unaffected following spherical Ag NP treatment.…”

Section: Input Of Nanomaterials Towards Callus Induction Organogenesmentioning

confidence: 99%

Nanomaterials in plant tissue culture: the disclosed and undisclosed

2017

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“…Since plants are recognized as the producers in food chain, these organisms are significant component of ecological system and it is important to know different nanoparticles influence and their interaction with plant organisms [19]. Previous investigations showed that different types, sizes, concentrations, and properties of NPs variously affect different plant species [20][21][22][23][24]. Iron NPs are common in the environment despite the fact that they are unstable in the presence of oxygen [25].…”

Section: +mentioning

confidence: 99%

“…Somatic embryogenesis is widely accepted by scientists as efficient method for plant micropropagation or genetic improvement which has the crucial role in plant production on the large scale [21,28,29]. Therefore, it is important to obtain somatic embryos on in vitro plant cultures [30].…”

Section: +mentioning

confidence: 99%

Plant Explants Grown on Medium Supplemented with Fe₃O₄ Nanoparticles Have a Significant Increase in Embryogenesis

Kokina

Mickeviča

Jahundoviča

et al. 2017

Journal of Nanomaterials

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Development of nanotechnology leads to the increasing release of nanoparticles in the environment that results in accumulation of different NPs in living organisms including plants. This can lead to serious changes in plant cultures which leads to genotoxicity. The aims of the present study were to detect if iron oxide NPs pass through the flax cell wall, to compare callus morphology, and to estimate the genotoxicity in Linum usitatissimum L. callus cultures induced by different concentrations of Fe 3 O 4 nanoparticles. Two parallel experiments were performed: experiment A, where flax explants were grown on medium supplemented with 0.5 mg/l, 1 mg/l, and 1.5 mg/l Fe 3 O 4 NPs for callus culture obtaining, and experiment B, where calluses obtained from basal MS medium were transported into medium supplemented with concentrations of NPs identical to experiment A. Obtained results demonstrate similarly in both experiments that 25 nm Fe 3 O 4 NPs pass into callus cells and induce low toxicity level in the callus cultures. Nevertheless, calluses from experiment A showed 100% embryogenesis in comparison with experiment B where 100% rhizogenesis was noticed. It could be associated with different stress levels and adaptation time for explants and calluses that were transported into medium with Fe 3 O 4 NPs supplementation.

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Reaction of Flax (Linum Usitatissimum L.) Calli Culture to Supplement of Medium by Carbon Nanoparticles

Cited by 10 publications

References 65 publications

Case Study of Somaclonal Variation in Resistance GenesMloandPme3in Flaxseed (Linum usitatissimumL.) Induced by Nanoparticles

Case Study of Somaclonal Variation in Resistance GenesMloandPme3in Flaxseed (Linum usitatissimumL.) Induced by Nanoparticles

Nanomaterials in plant tissue culture: the disclosed and undisclosed

Plant Explants Grown on Medium Supplemented with Fe₃O₄ Nanoparticles Have a Significant Increase in Embryogenesis

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Reaction of Flax (Linum Usitatissimum L.) Calli Culture to Supplement of Medium by Carbon Nanoparticles

Cited by 10 publications

References 65 publications

Case Study of Somaclonal Variation in Resistance GenesMloandPme3in Flaxseed (Linum usitatissimumL.) Induced by Nanoparticles

Case Study of Somaclonal Variation in Resistance GenesMloandPme3in Flaxseed (Linum usitatissimumL.) Induced by Nanoparticles

Nanomaterials in plant tissue culture: the disclosed and undisclosed

Plant Explants Grown on Medium Supplemented with Fe3O4 Nanoparticles Have a Significant Increase in Embryogenesis

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Product

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Plant Explants Grown on Medium Supplemented with Fe₃O₄ Nanoparticles Have a Significant Increase in Embryogenesis