Reaction of cultured pulp cells to eight different cements based on glass ionomers

Müller, Jan-Dirk; Brückner, Gert; Kraft, Erik; Hörz, Wolfram

doi:10.1016/0109-5641(90)90024-9

Cited by 32 publications

(12 citation statements)

References 18 publications

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“…Thus, this material appears to be more prone to leaching of soluble components, including both F and other agents which could be responsible for the cytotoxicity. This supports the statement of Muller et al (1990) that the cytotoxicity of the GICs they tested was not due to F; rather, it was attributed to other unidentified toxic components diffusing into the culture medium. Conventional GICs release a range of other ions (Kawahara et al, 1979;Matsuya et al, 1984;Wilson et al, 1985;Bapna and Mueller, 1994).…”

Section: Discussionsupporting

confidence: 84%

Variability in Cytotoxicity and Fluoride Release of Resin-modified Glass-ionomer Cements

Kan

Messer

1997

J Dent Res

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New-generation glass-ionomer cements contain resin to improve their restorative properties. These resin-modified glass-ionomer cements vary considerably in their chemistry, which could result in corresponding variability in their physical and biological properties. This study investigated the cytotoxicity and the fluoride release of two resin-modified glass ionomers, a conventional glass-ionomer cement, and a resin composite. Samples were prepared and extracted in distilled water for 1, 4, and 7 days; eluates were filtered and tested by means of 3T3 mouse fibroblasts. Cytotoxicity (MTT assay) values were low for all materials and extraction times, indicating minimal cytotoxicity of all materials (less than 30% inhibition). Cytotoxicity of one resin-modified glass ionomer was significantly higher than for the other materials (p < 0.001). One resin-modified glass ionomer and the conventional glass-ionomer cement released significantly more fluoride at each time interval (p < 0.001) than the other resin-modified glass-ionomer cement and the resin composite. Fluoride release and cytotoxicity were correlated (r2 = 0.60; p < 0.001), although the fluoride release does not account for the cytotoxicity observed. Cytotoxicity and fluoride release suggest that one hybrid behaved more like a conventional glass ionomer, and the other like a resin composite. These differences may have implications for material selection in specific clinical situations.

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Section: Discussionsupporting

confidence: 84%

Variability in Cytotoxicity and Fluoride Release of Resin-modified Glass-ionomer Cements

Kan

Messer

1997

J Dent Res

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“…In vitro assessments of a broad range of chemically cured GICs have shown that many of the materials released chemicals that were highly toxic to mammalian cells in the hours and days after mixing (Meryon, Stephens & Browne 1983;Müller et al . 1990).…”

Section: Discussionmentioning

confidence: 99%

Component release from light‐activated glass ionomer and compomer cements

Hamid

Okamoto

Iwaku

et al. 1998

J of Oral Rehabilitation

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The purpose of this study was to identify and quantify any component released from seven commercially available light-cured or resin-modified glass ionomer and compomer cements. Twenty-one separate cylindrical stainless steel moulds 6 mm in diameter and 1.0 mm deep were filled with one of seven glass ionomer or compomer cements, light activated and then immediately immersed in separate containers of distilled water. Water samples were retrieved over a time period of up to 30 days and retained for analysis. An occlusal cavity 6 mm in diameter was prepared in extracted human third molar teeth with a remaining dentin thickness of 1.6-2.0 mm. A polypropylene chamber was attached to the cemento-enamel junction of each tooth to contain 1 mL of distilled water. Ten teeth were each filled with one of three cements and light activated. Water samples (eluates) were retrieved over a period of time. All samples were analysed by high performance liquid chromatography. Only one component, hydroxyethyl methacrylate (HEMA), was detected in the eluates from both tooth and mould samples. Analysis of diffusion of the HEMA through dentin showed a relatively sustained movement into the pulp space during the first day, with exponential decline thereafter. Our data show that HEMA was released from all of the light activated glass ionomer cements studied and from the compomer, both directly into water and through dentin. This release may be relevant both to the risk of adverse pulpal responses in patients and to the risk of allergy in patients and dental personnel.

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“…According to the results of this study, a traditional GIC exhibited the least toxicity, and cell viability was approximately 90% in this GIC group. The cytotoxicity of conventional GICs has been investigated in different studies [19][20][21] using different cell types. Interestingly, one specific GIC product, Ketac Cem TM , caused no alterations in cell morphology, but inhibited RNA and protein synthesis of the treated gingival fibroblasts.…”

Section: Discussionmentioning

confidence: 99%

Cytotoxic effects of resin-modified orthodontic band adhesives

Malkoç¹,

Çörekçi²,

Botsali³

et al. 2010

The Angle Orthodontist

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) were prepared and the samples were extracted in 3 mL of Basal Medium Eagle with 10% newborn calf serum for 24 hours. The L929 cells were plated (25,000 cells/mL) in wells of 96-well dishes and maintained in a humidified incubator for 24 hours at 37uC, 5% CO 2 , and 95% air. After 24-hour incubation of the cells, the incubation medium was replaced by the immersed medium in which the samples were stored. Then L929 cells were incubated in contact with eluates for 24 hours. The cell mitochondrial activity was evaluated by the methyltetrazolium test. Twelve wells were used for each specimen, and methyltetrazolium tests were applied two times. The data were statistically analyzed using one-way analysis of variance and Tukey Honestly Significantly Different tests. Results:Results with L929 fibroblasts demonstrated that all freshly prepared resin-modified orthodontic band adhesive materials reduced vital cell numbers (P . .05), in comparison to the control group. Our data demonstrate that all materials showed significant cytotoxicity compared to the control group. Conclusions:The results indicate that all materials showed significant cytotoxicity compared to the control group, and further studies using different test methods are needed for all resin-modified orthodontic band adhesives. (Angle Orthod. 2010;80:890-895.)

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Reaction of cultured pulp cells to eight different cements based on glass ionomers

Cited by 32 publications

References 18 publications

Variability in Cytotoxicity and Fluoride Release of Resin-modified Glass-ionomer Cements

Variability in Cytotoxicity and Fluoride Release of Resin-modified Glass-ionomer Cements

Component release from light‐activated glass ionomer and compomer cements

Cytotoxic effects of resin-modified orthodontic band adhesives

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