Re-evaluation of the ferrous oxidation in xylenol orange assay for the measurement of plasma lipid hydroperoxides

Södergren, Eva; Nourooz‐Zadeh, Jaffar; Berglund, Lars; Vessby, Bengt

doi:10.1016/s0165-022x(98)00025-6

“…The relatively narrow CI indicates the sufficient power of this study to detect clinically significant differences. However, it should be noted that there is a large inter-individual variation in the degree of loss of detectable hydroperoxides Rapeseed oil-based fats and oxidative stress E Södergren et al after storage at 770 C (Södergren et al, 1998) and that our results from the measurements of hydroperoxides in samples stored frozen should be interpreted cautiously. In contrast to the rapeseed oil-based diets reported above, diets containing a higher proportion of PUFA and less MUFA suggest an increased lipid peroxidation with increased urinary excretion of F 2 -isoprostanes as measured by radioimmunoassay (Turpeinen et al, 1998), plasma TBARS formation (Berry et al, 1991), and urinary TBARS levels (Jenkinson et al, 1999) in healthy volunteers.…”

Section: Discussionmentioning

confidence: 61%

“…Total plasma hydroperoxide concentrations were measured using the ferrous oxidation in xylenol orange, version 2 (FOX2) assay as described previously by Södergren et al (1998). In brief, for each sample, aliquots of plasma were both incubated (30 min, 20 -25 C) in triphenylphosphine to remove hydroperoxides to generate a blank and in methanol to generate a test sample.…”

Section: Biomarkers Of Lipid Peroxidationmentioning

confidence: 99%

A diet containing rapeseed oil-based fats does not increase lipid peroxidation in humans when compared to a diet rich in saturated fatty acids

Södergren

¹

,

Gustafsson

²

,

Basu

³

et al. 2001

View full text Add to dashboard Cite

Objective: To compare the effects of a rapeseed oil-based diet containing an increased proportion of easily oxidised polyunsaturated fatty acids such as a-linolenic acid with a diet rich in saturated fatty acids on the degree of lipid peroxidation in the human body. Design: A randomised cross-over study. Subjects and interventions: Nineteen healthy moderately hyperlipidemic subjects (six women and 13 men, age 50 AE 8 y and body mass index (BMI) 24.5 AE 2.6 kg=m 2 ) were given a rapeseed oil-based diet (RO) and a control diet (SAT) rich in saturated fatty acids during two consecutive 4 week periods separated by a 4 week wash-out period. Biomarkers of lipid peroxidation and antioxidants were analysed in plasma and urine. Results: No significant differences in plasma or urinary levels of free 8-iso-prostaglandin F 2a , plasma total 8-isoprostaglandin F 2a plasma hydroperoxides or plasma malondialdehyde were observed between the RO and SAT diets (P ¼ 0.14 -0.95). A higher concentration of serum g-tocopherol was detected after the RO diet compared to the SAT diet (P < 0.001), whereas the serum a-tocopherol concentration and plasma antioxidative capacity did not differ between the two test diets. The total cholesterol, LDL cholesterol and LDL=HDL ratio were lower after the RO diet compared to the SAT diet (P < 0.001), while HDL cholesterol and total triglyceride levels were similar after the two diets. Conclusion: These results suggest that a rapeseed oil-based diet rich in a-linolenic acid does not seem to increase the degree of lipid peroxidation in plasma and urine compared to a diet rich in saturated fats. This is possibly due to a sufficient content of antioxidants in the rapeseed oil diet to increase circulating concentrations of antioxidants that may protect unsaturated fatty acids from oxidation.

show abstract

“…After 30 min, 900 ml of FOX reagent (500 ml of 1 mM ferrous-ammonium sulfate, 200 ml of 0.25 M sulfuric acid in methanol, 200 ml of 1 mM xylenol orange in methanol) was added. Absorbance from cuvettes containing TPP was measured after 30 min, and absorbance from cuvettes without TPP was assessed after 116 h. As TPP is a specific reducing agent of organic hydroperoxides, the residual absorbance developed by cuvettes containing TPP measures hydrogen peroxide, free ferric ion or other chromophores present in the sample (Nourooz-Zadeh et al, 1994;Sodergren et al, 1998;VanderJagt et al, 2001). Therefore, the difference in absorbance between cuvettes without TPP and with TPP was used to calculate the LHP value by means of a calibration curve, using CHP as standard.…”

Section: Lipid Hydroperoxide Determinationmentioning

confidence: 99%

Dietary n-6- or n-3-rich vegetable fats and α-tocopheryl acetate: effects on fatty acid composition and stability of rabbit plasma, liver and meat

Tres

¹

,

Bou

²

,

Codony

³

et al. 2009

Animal

View full text Add to dashboard Cite

We supplemented diets with a-tocopheryl acetate (100 mg/kg) and replaced beef tallow (BT) in feeds with increasing doses of n-6-or n-3-rich vegetable fat sources (linseed and sunflower oil), and studied the effects on the fatty acid (FA) composition, the a-tocopherol (aT) content and the oxidative stability of rabbit plasma and liver. These effects were compared with those observed in a previous study in rabbit meat. As in meat, the content of saturated, monounsaturated and trans FA in plasma and liver mainly reflected feed FA profile, except stearic acid in liver, which increased as feeds contained higher doses of vegetable fat, which could be related to an inhibition of the activity of the stearoyl-CoA-desaturase. As linseed oil increased in feeds, the n-6/n-3 FA ratio was decreased in plasma and liver as a result of the incorporation of FA from diets and also, due to the different performance and selectivity of desaturase enzymes. However, an increase in the dose of vegetable fat in feeds led to a significant reduction in the aT content of plasma and liver, which was greater when the fat source was linseed oil. Increasing the dose of vegetable fat in feeds also led to an increase in the susceptibility to oxidation (lipid hydroperoxide (LHP) value) of rabbit plasma, liver and meat and on the thiobarbituric acid (TBA) values of meat. Although the dietary supplementation with a-tocopheryl acetate increased the aT content in plasma and liver, it did not modify significantly their TBA or LHP values. In meat however, both TBA and LHP values were reduced by the dietary supplementation with a-tocopheryl acetate. The plasma aT content reflected the aT content in tissues, and correlated negatively with tissue oxidability. From the studied diets, those containing 1.5% linseed oil plus 1.5% BT and 100 mg of a-tocopheryl acetate/kg most improved the FA composition and the oxidative stability of rabbit tissues.

show abstract

“…The relatively narrow CI indicates the sufficient power of this study to detect clinically significant differences. However, it should be noted that there is a large inter-individual variation in the degree of loss of detectable hydroperoxides Rapeseed oil-based fats and oxidative stressafter storage at 770 C (Södergren et al, 1998) and that our results from the measurements of hydroperoxides in samples stored frozen should be interpreted cautiously. In contrast to the rapeseed oil-based diets reported above, diets containing a higher proportion of PUFA and less MUFA suggest an increased lipid peroxidation with increased urinary excretion of F 2 -isoprostanes as measured by radioimmunoassay (Turpeinen et al, 1998), plasma TBARS formation (Berry et al, 1991), and urinary TBARS levels (Jenkinson et al, 1999) in healthy volunteers.…”

Section: Discussionmentioning

confidence: 77%

A diet containing rapeseed oil-based fats does not increase lipid peroxidation in humans when compared to a diet rich in saturated fatty acids

Södergren¹,

Gustafsson²,

Basu³

et al. 2024

Eur J Clin Nutr

1

0

View full text Add to dashboard Cite

Objective: To compare the effects of a rapeseed oil-based diet containing an increased proportion of easily oxidised polyunsaturated fatty acids such as a-linolenic acid with a diet rich in saturated fatty acids on the degree of lipid peroxidation in the human body. Design: A randomised cross-over study. Subjects and interventions: Nineteen healthy moderately hyperlipidemic subjects (six women and 13 men, age 50 AE 8 y and body mass index (BMI) 24.5 AE 2.6 kg=m 2 ) were given a rapeseed oil-based diet (RO) and a control diet (SAT) rich in saturated fatty acids during two consecutive 4 week periods separated by a 4 week wash-out period. Biomarkers of lipid peroxidation and antioxidants were analysed in plasma and urine. Results: No significant differences in plasma or urinary levels of free 8-iso-prostaglandin F 2a , plasma total 8-isoprostaglandin F 2a plasma hydroperoxides or plasma malondialdehyde were observed between the RO and SAT diets (P ¼ 0.14 -0.95). A higher concentration of serum g-tocopherol was detected after the RO diet compared to the SAT diet (P < 0.001), whereas the serum a-tocopherol concentration and plasma antioxidative capacity did not differ between the two test diets. The total cholesterol, LDL cholesterol and LDL=HDL ratio were lower after the RO diet compared to the SAT diet (P < 0.001), while HDL cholesterol and total triglyceride levels were similar after the two diets. Conclusion: These results suggest that a rapeseed oil-based diet rich in a-linolenic acid does not seem to increase the degree of lipid peroxidation in plasma and urine compared to a diet rich in saturated fats. This is possibly due to a sufficient content of antioxidants in the rapeseed oil diet to increase circulating concentrations of antioxidants that may protect unsaturated fatty acids from oxidation.

show abstract

Re-evaluation of the ferrous oxidation in xylenol orange assay for the measurement of plasma lipid hydroperoxides

Cited by 135 publications

References 36 publications

A diet containing rapeseed oil-based fats does not increase lipid peroxidation in humans when compared to a diet rich in saturated fatty acids

A diet containing rapeseed oil-based fats does not increase lipid peroxidation in humans when compared to a diet rich in saturated fatty acids

Dietary n-6- or n-3-rich vegetable fats and α-tocopheryl acetate: effects on fatty acid composition and stability of rabbit plasma, liver and meat

A diet containing rapeseed oil-based fats does not increase lipid peroxidation in humans when compared to a diet rich in saturated fatty acids

Contact Info

Product

Resources

About