Re-Evaluation of Imaging Methods of Reactive Oxygen and Nitrogen Species in Plants and Fungi: Influence of Cell Wall Composition

This article contains data related to the research article entitled, “Organic radical imaging in plants: Focus on protein radicals” (Kumar et al., 2018). The data presented herein focus on reactive oxygen species (ROS) and organic radical formed within photosynthetic tissues of Arabidopsis thaliana during high light stress and includes (1) Confocal laser scanning microscopic images using 3′-p-(hydroxyphenyl) fluorescein (HPF) as specific probe for the detection of hydroxyl radical (HO•); (2) Confocal laser scanning microscopic images using Singlet Oxygen Sensor Green (SOSG) as a specific probe for the detection of singlet oxygen (1O2) and; (3) Electron paramagnetic resonance (EPR) spectroscopy using spin traps for the detection of organic radical.

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“…Fig. 2 A shows a schematic representation of the experimental procedure for details refer to references [2] , [3] .…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

Data on detection of singlet oxygen, hydroxyl radical and organic radical in Arabidopsis thaliana

et al. 2018

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Biological autoluminescence as a perturbance-free method for monitoring oxidation in biosystems

Vahalová

Cifra

2023

Progress in Biophysics and Molecular Biology

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Singlet oxygen imaging using fluorescent probe Singlet Oxygen Sensor Green in photosynthetic organisms

Prasad

Sedlářová

Pospı́šil

2018

Sci Rep

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Formation of singlet oxygen (1O2) was reported to accompany light stress in plants, contributing to cell signaling or oxidative damage. So far, Singlet Oxygen Sensor Green (SOSG) has been the only commercialized fluorescent probe for 1O2 imaging though it suffers from several limitations (unequal penetration and photosensitization) that need to be carefully considered to avoid misinterpretation of the analysed data. Herein, we present results of a comprehensive study focused on the appropriateness of SOSG for 1O2 imaging in three model photosynthetic organisms, unicellular cyanobacteria Synechocystis sp. PCC 6803, unicellular green alga Chlamydomonas reinhardtii and higher plant Arabidopsis thaliana. Penetration of SOSG differs in both unicellular organisms; while it is rather convenient for Chlamydomonas it is restricted by the presence of mucoid sheath of Synechocystis, which penetrability might be improved by mild heating. In Arabidopsis, SOSG penetration is limited due to tissue complexity which can be increased by pressure infiltration using a shut syringe. Photosensitization of SOSG and SOSG endoperoxide formed by its interaction with 1O2 might be prevented by illumination of samples by a red light. When measured under controlled conditions given above, SOSG might serve as specific probe for detection of intracellular 1O2 formation in photosynthetic organisms.

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Re-Evaluation of Imaging Methods of Reactive Oxygen and Nitrogen Species in Plants and Fungi: Influence of Cell Wall Composition

Cited by 5 publications

References 54 publications

Data on detection of singlet oxygen, hydroxyl radical and organic radical in Arabidopsis thaliana

Data on detection of singlet oxygen, hydroxyl radical and organic radical in Arabidopsis thaliana

Biological autoluminescence as a perturbance-free method for monitoring oxidation in biosystems

Singlet oxygen imaging using fluorescent probe Singlet Oxygen Sensor Green in photosynthetic organisms

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