Data on detection of singlet oxygen, hydroxyl radical and organic radical in Arabidopsis thaliana

Kumar, Aditya; Prasad, Ankush; Sedlářová, Michaela; Pospı́šil, Pavel

doi:10.1016/j.dib.2018.11.033

Cited by 12 publications

(10 citation statements)

References 5 publications

(8 reference statements)

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“…Mechanical injury of Arabidopsis leaves was carried out using a sharp razor blade. Leaf pieces of ca 5 × 5 mm were cut out in HEPES buffer (pH 7.5) and infiltrated with fluorochromes using a syringe (see protocol in references) (Kumar et al, 2018;Prasad et al, 2018). Following 30 min incubation in desired probe (100mM/250mM DHE, 50 mM Spy-LHP, or 50 mM SOSG), tissues were transferred into HEPES buffer on a glass slide and visualized by Fluorview 1000 confocal laser scanning microscope (Olympus Czech Group, Prague, Czech Republic).…”

Section: Sample Preparation and Confocal Laser Scanning Microscopymentioning

confidence: 99%

“…The peroxyl radicals are reactive intermediates and are known to be associated with the propagation part of the lipid peroxidation (Miyamoto et al, 2007). The lipid peroxidation in the biological membranes is the most obvious symptoms visible in plants as an outcome of oxidative stress in plants (Jarvis, 2011;Kumar et al, 2018;Zhang et al, 2018). The high energy intermediates (dioxetanes and tetroxide) formed during the oxidative radical reactions decompose to triplet carbonyls ( 3 C=O*) which can then transfer triplet energy to molecular oxygen creating 1 O 2 (Di Mascio et al, 1992;Miyamoto et al, 2003;Miyamoto et al, 2007;Cifra and Pospíšil, 2014;Pospíšil et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

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Reactive Oxygen Species as a Response to Wounding: In Vivo Imaging in Arabidopsis thaliana

et al. 2020

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Mechanical injury or wounding in plants can be attributed to abiotic or/and biotic causes. Subsequent defense responses are either local, i.e. within or in the close vicinity of affected tissue, or systemic, i.e. at distant plant organs. Stress stimuli activate a plethora of early and late reactions, from electric signals induced within seconds upon injury, oxidative burst within minutes, and slightly slower changes in hormone levels or expression of defense-related genes, to later cell wall reinforcement by polysaccharides deposition, or accumulation of proteinase inhibitors and hydrolytic enzymes. In the current study, we focused on the production of reactive oxygen species (ROS) in wounded Arabidopsis leaves. Based on fluorescence imaging, we provide experimental evidence that ROS [superoxide anion radical (O 2 •−) and singlet oxygen (1 O 2)] are produced following wounding. As a consequence, oxidation of biomolecules is induced, predominantly of polyunsaturated fatty acid, which leads to the formation of reactive intermediate products and electronically excited species.

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Section: Sample Preparation and Confocal Laser Scanning Microscopymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Reactive Oxygen Species as a Response to Wounding: In Vivo Imaging in Arabidopsis thaliana

et al. 2020

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“…Formation of 1 O 2 and HO ∙ in leaves was visualized by confocal laser scanning microscopy (Fluorview 1000 unit attached to IX80 microscope; Olympus Czech Group, Prague, Czechia) (Kumar et al, 2018; Prasad et al, 2018). Leaf pieces were incubated either in dark or red light (1000 μmol photons m –2 s –1 ) at room temperature with 50 μM Singlet Oxygen Sensor Green (SOSG) for the detection of 1 O 2 and 5 μM Hydroxy Phenyl Fluorescein (HPF) for the detection of HO ∙ in the presence of HEPES buffer (pH 7.5).…”

Section: Methodsmentioning

confidence: 99%

Characterization of Protein Radicals in Arabidopsis

et al. 2019

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Oxidative modification of proteins in photosystem II (PSII) exposed to high light has been studied for a few decades, but the characterization of protein radicals formed by protein oxidation is largely unknown. Protein oxidation is induced by the direct reaction of proteins with reactive oxygen species known to form highly reactive protein radicals comprising carbon-centered (alkyl) and oxygen-centered (peroxyl and alkoxyl) radicals. In this study, protein radicals were monitored in Arabidopsis exposed to high light by immuno-spin trapping technique based on the detection of 5,5-dimethyl-1-pyrroline N-oxide (DMPO) nitrone adducts using the anti-DMPO antibody. Protein radicals were imaged in Arabidopsis leaves and chloroplasts by confocal laser scanning microscopy using fluorescein conjugated with the anti-DMPO antibody. Characterization of protein radicals by standard blotting techniques using PSII protein specific antibodies shows that protein radicals are formed on D1, D2, CP43, CP47, and Lhcb3 proteins. Protein oxidation reflected by the appearance/disappearance of the protein bands reveals that formation of protein radicals was associated with protein fragmentation (cleavage of the D1 peptide bonds) and aggregation (cross-linking with another PSII subunits). Characterization of protein radical formation is important for better understating of the mechanism of oxidative modification of PSII proteins under high light.

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“…If the production of ROS is initiated, for example, during a stress response as discussed above, the generation of ·OH is likely to proceed. Hydroxyl radicals can be detected in plant cells [ 77 , 78 ], and have been found to have multiple effects.…”

Section: Downstream Effectsmentioning

confidence: 99%

Downstream Signalling from Molecular Hydrogen

Hancock

Russell

2021

Plants

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Molecular hydrogen (H2) is now considered part of the suite of small molecules that can control cellular activity. As such, H2 has been suggested to be used in the therapy of diseases in humans and in plant science to enhance the growth and productivity of plants. Treatments of plants may involve the creation of hydrogen-rich water (HRW), which can then be applied to the foliage or roots systems of the plants. However, the molecular action of H2 remains elusive. It has been suggested that the presence of H2 may act as an antioxidant or on the antioxidant capacity of cells, perhaps through the scavenging of hydroxyl radicals. H2 may act through influencing heme oxygenase activity or through the interaction with reactive nitrogen species. However, controversy exists around all the mechanisms suggested. Here, the downstream mechanisms in which H2 may be involved are critically reviewed, with a particular emphasis on the H2 mitigation of stress responses. Hopefully, this review will provide insight that may inform future research in this area.

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Data on detection of singlet oxygen, hydroxyl radical and organic radical in Arabidopsis thaliana

Cited by 12 publications

References 5 publications

Reactive Oxygen Species as a Response to Wounding: In Vivo Imaging in Arabidopsis thaliana

Reactive Oxygen Species as a Response to Wounding: In Vivo Imaging in Arabidopsis thaliana

Characterization of Protein Radicals in Arabidopsis

Downstream Signalling from Molecular Hydrogen

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