eThe retinoblastoma tumor suppressor gene (RB) product has been implicated in epigenetic control of gene expression owing to its ability to physically bind to many chromatin modifiers. However, the biological and clinical significance of this activity was not well elucidated. To address this, we performed genetic and epigenetic analyses in an Rb-deficient mouse thyroid C cell tumor model. Here we report that the genetic interaction of Rb and ATM regulates DNMT1 protein stability and hence controls the DNA methylation status in the promoters of at least the Ink4a, Shc2, FoxO6, and Noggin genes. Furthermore, we demonstrate that inactivation of pRB promotes Tip60 (acetyltransferase)-dependent ATM activation; allows activated ATM to physically bind to DNMT1, forming a complex with Tip60 and UHRF1 (E3 ligase); and consequently accelerates DNMT1 ubiquitination driven by Tip60-dependent acetylation. Our results indicate that inactivation of the pRB pathway in coordination with aberration in the DNA damage response deregulates DNMT1 stability, leading to an abnormal DNA methylation pattern and malignant progression.T he retinoblastoma tumor suppressor gene (RB) is prevalently mutated at the initiation of retinoblastoma, osteosarcoma, and small-cell lung cancer. However, in the majority of human cancers, retinoblastoma protein (pRB) inactivation is frequently detected during tumor progression and is implicated in various facets of malignant behaviors (1). This suggests that pRB may possess more functions beyond its well-known roles in the control of the cell cycle and differentiation (2, 3). Previous studies demonstrated the direct interaction of pRB with a number of epigenetic modifiers carrying the LXCXE motif, including DNA methyltransferase 1 (DNMT1) (4, 5). pRB has been suggested to play a certain role in nuclear reprogramming in embryonic stem (ES) cells, induced pluripotent stem (iPS) cells, mouse embryonic fibroblasts (MEFs), as well as tumor cells (6-10). Furthermore, RB loss was recently shown to contribute to the progression of human retinoblastoma via epigenetic modifications (11). However, the precise mechanism and target genes of the pRB epigenetic function are still largely unknown.pRB inactivation induces DNA double-strand breaks (DSBs) via multiple pathways (12-17). The MRN (Mre11, Rad50, and NBS1) sensor complex detects DSBs, rapidly activates ataxia telangiectasia mutated (ATM), and recruits ATM to the site of DSBs. ATM then activates a number of transducers, such as MDC1, 53BP1, and BRCA1, and effector kinases, such as Chk2. These proteins subsequently activate molecules required for DSB repair, including the histone variant H2AX (18)(19)(20). Our previous study demonstrated that some components of the DNA damage response (DDR) are activated upon Rb loss in vivo and in vitro (15). We therefore speculated that ATM might mediate pRB functions to regulate the DDR pathway.C cell (calcitonin-producing cell) tumors developed in Rb ϩ/Ϫ mice are precancerous and display many features of the DDR and cellular s...