Raw donkey milk as a source of Enterococcus diversity: Assessment of their technological properties and safety characteristics

Aspri, Maria; Bozoudi, Despina; Tsaltas, Dimitrios; Hill, Colin; Papademas, Photis

doi:10.1016/j.foodcont.2016.05.022

Cited by 45 publications

(42 citation statements)

References 63 publications

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“…Finally, none of the isolates was positive for vanA and vanB genes. This is in agreement with the results from previous studies [24,43], reporting that vanA and vanB genes have not been found frequently in enterococci isolates from food sources. However, in other work, it has been reported that three strains (50%) of Enterococcus faecalis were positive to vanA gene [22].…”

Section: Pathogenicitysupporting

confidence: 93%

“…A good mesophilic fast acid producing starter culture will reduce the pH of the milk at 5.3 after 6 h of incubation at 37 °C [24]. Of the Enterococci of the present study, none of them reduced the milk pH to <6.0 after 6 h of incubation ( Figure 1, Table 5).…”

Section: Acidification Activitymentioning

confidence: 52%

“…All the selected strains were checked for the presence of genes encoding virulence, antibiotic resistance and amino acid decarboxylase activity [23,24]. DNA was extracted using Bacterial DNA kit (Macherey-Nagel, Duren, Germany) according to the manufacturer's instructions, following by amplifications of genes described in Table 3 using specific primers.…”

Section: Virulence Activity Using Genotypic Testsmentioning

confidence: 99%

“…Five (7.8%) of our isolates were positive for gelE gene. However, in previous studies, it has been found that strains having this gene did not produce gelatinase [24,38], because the fsr operon could be damaged, lost, or suffer deletions due to physiological stresses from laboratory storage.. Hemolysin production increases the severity of enterococcal infections, and the presence of hemolysin/cytolysin genes is considered a risk pathogenicity factor. Regarding the cylA gene, two (3.1%) isolates gave a positive result, but according to phenotypical tests, only γ-hemolysis was observed.…”

Section: Pathogenicitymentioning

confidence: 99%

“…Regarding the cylA gene, two (3.1%) isolates gave a positive result, but according to phenotypical tests, only γ-hemolysis was observed. cylA has been considered as a "silent gene" and its gene expression can be influenced by the environmental factors and conditions used for phenotypic tests [24]. The genes esp, efaA, and ace have been involved in the colonization and adhesion at biotic and non-biotic surfaces, and the host immune system evasion.…”

Section: Pathogenicitymentioning

confidence: 99%

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Enterococci Isolated from Cypriot Green Table Olives as a New Source of Technological and Probiotic Properties

2018

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Table olive is one of the main fermented vegetable worldwide and can be processed as treated or natural product. Lactic Acid Bacteria (LAB) are responsible for the fermentation of treated olives. The aim of this work was to study the technological characteristics and the potential probiotic properties of LAB isolated from Cypriot green table olives. This is the first comprehensive report on the isolation and characterization of LAB isolates retrieved from Cypriot green table olives. From a collection of 92 isolates from spontaneously fermenting green olives, 64 g positive isolates were firstly identified to genus level using biochemical tests, and secondly to species level using multiplex species specific polymerase chain reaction (PCR) amplifications of the sodA gene. Moreover, each of our isolates were tested for their technological and probiotics properties, as well as for their safety characteristics, using biochemical and molecular methods, in order to be used as starter cultures. Finally, to discriminate the most promising isolates on the base of their technological and probiotics properties, Principal component analysis was used. All the isolates were identified as Enteroccocus faecium, having interesting technological properties, while pathogenicity determinants were absent. Principal component analysis showed that some isolates had a combination of the tested parameters. These findings demonstrate that enteroccoci from Cypriot table olives should be considered as a new source of potential starter cultures for fermented products, having possibly promising technological and probiotic attributes.

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Section: Pathogenicitysupporting

confidence: 93%

Section: Acidification Activitymentioning

confidence: 52%

Section: Virulence Activity Using Genotypic Testsmentioning

confidence: 99%

Section: Pathogenicitymentioning

confidence: 99%

Section: Pathogenicitymentioning

confidence: 99%

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Enterococci Isolated from Cypriot Green Table Olives as a New Source of Technological and Probiotic Properties

2018

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Probiotic Potential of Bacillus Strains Isolated from an Acidic Fermented Food Idli

Sharma

Devi

Ragul

et al. 2020

Probiotics & Antimicro. Prot.

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Psychobiotic Potential of Gamma-Aminobutyric Acid–Producing Marine Enterococcus faecium SH9 from Marine Shrimp

Sakkaa

Zaghloul

Ghanem

2022

Probiotics & Antimicro. Prot.

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Psychobiotics are a novel class of probiotics with potential to confer mental wellness via production of neuroactive compounds such as gamma-aminobutyric acid (GABA). The demand for new biological sources of GABA has increased steadily. Therefore, the current study reports the isolation of 17 presumptive lactic acid bacteria (LAB) from marine samples and their screening for GABA synthesis from monosodium glutamate (MSG) using thin-layer chromatography (TLC). The isolate SH9 was selected as a high GABA producing strain. The GABA content of SH9 cell free supernatant (CFS) was quantitatively determined by high performance liquid chromatography (HPLC) to be 0.97 g/L. SH9 was identified biochemically and molecularly as Enterococcus faecium (identity 99%). Moreover, SH9 demonstrated promising probiotic potentials; it gave no signs of hemolysis and could survive at low pH values and high bile salt concentrations. It also exhibited antimicrobial activity against highly pathogenic strains and the ability to grow at 6.5% NaCl. In addition, SH9 CFS showed anti-inflammatory and antioxidant properties. The glutamate decarboxylase (GAD) gene was detected in SH9 by using specific primers. Product of 540 bp was obtained, sequenced, and analyzed (accession number: MW713382). The inferred amino acid sequence was 99.3% identical to Lactobacillus plantarum M-6 gadB gene. The findings of this study suggest that the marine isolate E. faecium SH9 could be used as a novel psychobiotics in the development of GABA rich healthy products.

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Raw donkey milk as a source of Enterococcus diversity: Assessment of their technological properties and safety characteristics

Cited by 45 publications

References 63 publications

Enterococci Isolated from Cypriot Green Table Olives as a New Source of Technological and Probiotic Properties

Enterococci Isolated from Cypriot Green Table Olives as a New Source of Technological and Probiotic Properties

Probiotic Potential of Bacillus Strains Isolated from an Acidic Fermented Food Idli

Psychobiotic Potential of Gamma-Aminobutyric Acid–Producing Marine Enterococcus faecium SH9 from Marine Shrimp

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