Probiotic Potential of Bacillus Strains Isolated from an Acidic Fermented Food Idli

Sharma, Shivangi; Devi, Palanisamy Bruntha; Ragul, Kessavane; Shetty, Prathapkumar Halady

doi:10.1007/s12602-020-09650-x

Cited by 40 publications

(24 citation statements)

References 54 publications

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“…The results of this study indicated that the bacterial isolates remained stable at pH 2 and pH 3, without any substantial decline in viability. Previous studies reported that probiotic bacterial strains have been observed to tolerate pH 3 for 2.5 h [ 48 , 49 ]. In this study, pH adjusted at 3 was used to measure the acid tolerance capacity of Lactobacillus strains, as this is considered the standard pH to investigate the acid tolerance of probiotic strains in various studies [ 50 ].…”

Section: Discussionmentioning

confidence: 99%

Identification, Biochemical Characterization, and Safety Attributes of Locally Isolated Lactobacillus fermentum from Bubalus bubalis (buffalo) Milk as a Probiotic

Abid

Farid

Abid³

et al. 2022

Microorganisms

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The demand of functional foods is on the rise, and researchers are trying to develop nutritious dairy products by using well-characterized strains of bacteria. In this study, we identified locally isolated strains of Lactobacillus fermentum from Bubalus bubalis (Nilli Ravi buffalo) milk and evaluated their potential as probiotics in food products like fermented milk. Fifteen Lactobacillus strains were initially isolated, and only four strains (NMCC-2, NMCC-14, NMCC-17, and NMCC-27) were examined for morphological and biochemical characterizations due to their ability of gas production in Durham tubes. Moreover, these strains were selected for further probiotic characterizations due to their extreme morphological resemblance with lactic acid bacteria for their antimicrobial activity, enzymatic potential, autoaggregation capability, hydrophobicity, and acid and bile tolerance. All selected isolates showed significant probiotic potential. However, NMCC-14 and NMCC-17 strains showed maximum probiotic potential. The isolates (NMCC-2, NMCC-14, NMCC-17, and NMCC-27) were identified as Lactobacillus fermentum utilizing 16S rRNA gene sequencing. The in vivo safety study of NMCC-14 (dose: 1010 CFU/day/mice; 21 days, orally) showed no histological dysfunctions in a mouse model. Pathogenic bacterial enzymes reduced the beneficial bacterial load in the host gastrointestinal tract. These results suggest that the NMCC-14 strain is safe and can be potentially used as a probiotic. Moreover, fermented milk was prepared by using the NMCC-14 strain. The results revealed that NMCC-14 strain-based fermented milk had significantly (p < 0.05) higher protein content (4.4 ± 0.06), water-holding capacity (WHC), and dynamic viscosity as compared to non-fermented milk. The results suggest that L. fermentum NMCC-14 is safe and nontoxic; hence, it can be a beneficial supplement to be used for the development of dairy products to be subjected to further clinical testing.

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Section: Discussionmentioning

confidence: 99%

Identification, Biochemical Characterization, and Safety Attributes of Locally Isolated Lactobacillus fermentum from Bubalus bubalis (buffalo) Milk as a Probiotic

Abid

Farid

Abid³

et al. 2022

Microorganisms

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“…had DPPH scavenging activity of 2.57–27.34% ( Ragul et al, 2020 ). Also, some reports mentioned that intact cells of some probiotic strains expressed more DPPH scavenging activity than CFS ( Shivangi et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Characterization of Streptococcus salivarius as New Probiotics Derived From Human Breast Milk and Their Potential on Proliferative Inhibition of Liver and Breast Cancer Cells and Antioxidant Activity

et al. 2021

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Breast milk is well known as the abundant source of beneficial bacteria. A new alternative source of human probiotic origin from breast milk is in demand and currently of interest for both the functional food industry and biopharmaceuticals. The aim in this study was to investigate the anticancer and antioxidant efficacies of the new potential probiotics isolated from human breast milk. Three strains of lactic acid bacteria (LAB) have shown their potential probiotic criteria including antimicrobial activity, non-hemolytic property, and survival in acid and bile salt conditions. These strains showed high abilities on cell surface hydrophobicity, auto-aggregation, and co-aggregation. The genera identification by 16S rRNA sequencing and comparison revealed that they were Streptococcus salivarius BP8, S. salivarius BP156, and S. salivarius BP160. The inhibition of liver cancer cells (HepG2) and breast cancer cells (MCF-7) proliferation by these probiotic strains using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was 44.83–59.65 and 29.85–37.16%, respectively. The probiotic action mode was inducted via apoptotic mechanisms since they stimulate the liver and breast cancer cell death through DNA fragmentation and positive morphological changes by acridine orange (AO) and propidium iodide (PI) staining. The antioxidant activity of these probiotics in the form of intact cells, cell free supernatant (CFS), and heat-killed cells was evaluated by a 2,2–diphenyl–1–picrylhydrazyl (DPPH) assay, resulting in the scavenging activity rates of 16.93–25.43, 15.47–28.03, and 13.67–23.0%, respectively. These S. salivarius probiotic strains protected the L929 mouse fibroblasts against oxidative stress with very high survival rates at 94.04–97.77%, which was significantly higher (P < 0.05) than L-ascorbic acid at 75.89–78.67% in the control groups. The results indicated that S. salivarius BP8 and S. salivarius BP160 probiotic strains could be applied as functional foods or new alternative bioprophylactics for treating liver and breast cancers.

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“…Several studies have reported that in addition to the potential probiotic properties of Bacillus strains, the Bacillus exhibited strong anti-cholesterol, anti-bio lm, and antioxidant properties, making the strain with additional functional abilities (34). B. subtilis exerts an antimicrobial effect against a broad spectrum of pathogens through direct bactericidal activity or indirect enhancement of immune response, such as interrupting quorum-sensing regulatory system by production of fengycins (18), inhibiting S. aureus adhesion and bio lm formation by production of surfactant (35), and the enhancing anti-microbial function of macrophage (36).…”

Section: Discussionmentioning

confidence: 99%

Commensal Bacillus From Cow Milk Inhibits Staphylococcus Aureus Biofilm Formation and Mastitis in Mice

Qiu

Zhao

Feng

et al. 2021

Preprint

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Background: Mastitis, one of the most serious diseases in dairy industry, could cause tremendous economic losses worldwide and is commonly trigged by pathogen invasion. Staphylococcus aureus (S. aureus) -induced mastitis has been reported to play an important role in mastitis etiology characterized by high morbidity, recurrence, and increased antibiotic resistance, which may attribute to the formation of biofilm formation, a form of bacterial aggregation for better growth and resistance to adverse conditions. Probiotics Bacillus has been reported to disrupt bacteria quorum-sensing (QS) system, a central regulator for biofilm formation. However, whether commensal Bacillus affects S. aureus biofilm formation and consequent colonization during mastitis is still unknown. Results：Here, we identified that the Bacillus is associated with reduced colonization of S. aureus in the mammary gland of cows. Interestingly, Bacillus did not affect S. aureus growth but inhibited the biofilm formation of S. aureus by interfering with S. aureus QS signaling. The most obvious anti-biofilm effect was found in Bacillus subtilis H28, so it was selected for further study. We found that bacillus subtilis H28 treatment alleviated S. aureus-induced mastitis in mice, as showed by limiting pro-inflammatory cytokines production, enhancing barrier integrity, and reducing S. aureus burden. Consistently, Bacillus subtilis with the capacity to interfere S. aureus QS ameliorated S. aureus-induced NF-κB activation in mice mammary epithelial cells（MMECs）. Conclusions: Collectively, our results indicate that commensal Bacillus inhibits S. aureus colonization and alleviates S. aureus-induced mastitis by influencing biofilm formation, which suggests a potential strategy for the decolonization of S. aureus and acts as a basis for the prevention and treatment of S. aureus-related disease.

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Probiotic Potential of Bacillus Strains Isolated from an Acidic Fermented Food Idli

Cited by 40 publications

References 54 publications

Identification, Biochemical Characterization, and Safety Attributes of Locally Isolated Lactobacillus fermentum from Bubalus bubalis (buffalo) Milk as a Probiotic

Identification, Biochemical Characterization, and Safety Attributes of Locally Isolated Lactobacillus fermentum from Bubalus bubalis (buffalo) Milk as a Probiotic

Characterization of Streptococcus salivarius as New Probiotics Derived From Human Breast Milk and Their Potential on Proliferative Inhibition of Liver and Breast Cancer Cells and Antioxidant Activity

Commensal Bacillus From Cow Milk Inhibits Staphylococcus Aureus Biofilm Formation and Mastitis in Mice

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