Rattus norvegicus as a model for persistent renal colonization by pathogenic Leptospira interrogans

Athanazio, Daniel Abensur; Silva, Éverton F.; Santos, Cleiton Silva; Rocha, Gustavo Miranda; Vannier-Santos, Marcos A.; McBride, Alan J. A.; Ko, Albert I.; Reis, Mitermayer Galvão dos

doi:10.1016/j.actatropica.2007.10.012

Cited by 127 publications

(142 citation statements)

References 25 publications

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“…This study was able to isolate Leptospira interrogans serovar Copenhageni strain Fiocruz L1-130 (KO et al, 1999), and surveys conducted by KO et al (2007) and by ATHANAZIO et al (2008) emphasizes the important role of rodents in the epidemiological chain of leptospirosis, indicating their condition as reservoir of this strain to humans.…”

Section: Leptospiramentioning

confidence: 93%

“…In rural areas the transmission occurs more frequently because of defi ciency of good sanitary practices in animal handling. Surveys conducted by KO et al (2007) and by ATHANAZIO et al (2008) described the pathogenesis and the pathological changes caused by Leptospira interrogans serovar Copenhageni strain Fiocruz L1-130 (KO et al, 1999 in the kidneys of rats.…”

Section: Introductionmentioning

confidence: 99%

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Isolation of Leptospira spp. from a man living in a rural area of the Municipality of Cruz Alta, RS, Brazil

Silva

Löffler

et al. 2015

Cienc. Rural

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Section: Leptospiramentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Isolation of Leptospira spp. from a man living in a rural area of the Municipality of Cruz Alta, RS, Brazil

Silva

Löffler

et al. 2015

Cienc. Rural

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“…The pathogenesis of naturally-occurring leptospiral infection in rats has not been extensively studied and is complicated by a wide array of potentially confusing environmental factors that may also induce pathology in wild urban rats (11). The present study evaluated renal pathology in urban rats from a city where the prevalence of leptospiral isolation by kidney culture was approximately 20% (7).…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies evaluating renal morphology in captured urban rats carrying leptospires have all reported that interstitial nephritis was the principal change attributed to leptospires (9)(10)(11). The pathogenesis of naturally-occurring leptospiral infection in rats has not been extensively studied and is complicated by a wide array of potentially confusing environmental factors that may also induce pathology in wild urban rats (11).…”

Section: Discussionmentioning

confidence: 99%

Alteraciones histopatológicas en ratas naturalmente infectadas con Leptospira

et al. 2012

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Introduction:Histopathological changes by Leptospira in naturally infected rodent reservoirs have been poorly described. Objective: The aim of the current study is to describe renal histopathology associated with leptospirosis infection of naturally infected rodents captured in the urban area of the city of Medellin, Colombia. Materials and methods: We performed hematoxilin-eosin (H-E) on kidney samples collected from 254 captured rodents. The positive samples were processed by Warthin Starry (W-S) staining and PCRLipL32. Results: Fifty one rodent kidneys showed H-E histopathological changes that consisted of inflammatory infiltrate with lympho-plasmocitary cells and histiocytes. We performed W-S staining and PCR-LipL32 to 67 kidney samples, including the 51 that had shown detectable changes by H-E and 16 (8%) of 203 rodents with negative results. Eight of the samples that tested positive for H-E (15.7%) were also positive for W-S staining. All negative for H-E were also negative for W-S staining. Of the W-S positive samples also tested for culture only three tested positive for both. Additionally, 47 (92.1%) samples positive for H-E were positive for PCR; while eleven of the 16 (68.8%) negative for H-E were positive for PCR. The samples positive for PCR were subsequently tested for culture and 11 (23.4%) were positive. Seven samples were positive for PCR and W-S and three were positive for PCR, W-S and culture. All of the PCR-LipL32 fragments were sequenced and showed specific amplicons for L. interrogans. Conclusions:The Leptospira infection was confirmed in all of the animals tested. The only histological kidney lesion attributable to leptospiral infection in the reservoir was interstitial nephritis.

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“…Rats are resistant to acute disease and are more suited to studies focusing on mechanisms of persistent infection (3,13). The mouse model offers a broad array of immunological and genetic tools available for basic research; however, it has been poorly explored in leptospirosis.…”

mentioning

confidence: 99%

Attenuated Nephritis in Inducible Nitric Oxide Synthase Knockout C57BL/6 Mice and Pulmonary Hemorrhage in CB17 SCID and Recombination Activating Gene 1 Knockout C57BL/6 Mice Infected with Leptospira interrogans

et al. 2011

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The aims of this study were to investigate the frequency of pulmonary hemorrhage (PH) in mice unable to produce functional B and T lymphocytes and to explore the effect of an inducible nitric oxide synthase gene (Inos) knockout (KO) on the frequency/severity of interstitial nephritis in vivo. We studied the outcome of infection by the virulent Leptospira interrogans serovar Copenhageni strain Cop. The animals used were Inos KO mice, recombination activating gene 1 (Rag1) KO mice, CB17 severe combined immunodeficiency (SCID) mice, and the respective wild-type (WT) C57BL/6 and BALB/c controls. The Inos KO and WT mice survived with no clinical symptoms of leptospirosis. The frequency and severity of nephritis was significantly lower in the Inos KO mice. All of the Rag1 KO and SCID animals died of acute leptospirosis, whereas all of the WT mice survived. PH was observed in 57 and 94% of Rag1 KO mice and in 83 and 100% of SCID mice, using inoculum doses of 10 7 and 10 6 leptospires, respectively. There was no evidence of PH in the WT controls. In conclusion, the loss of the Inos gene had a negligible effect on the outcome of leptospiral infection, although we observed a reduced susceptibility for interstitial nephritis in this group. Of note, the absence of functional B-and T-cell lymphocytes did not preclude the occurrence of PH. These data provide evidence that PH in leptospirosis may not be related only to autoimmune mechanisms.

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Rattus norvegicus as a model for persistent renal colonization by pathogenic Leptospira interrogans

Cited by 127 publications

References 25 publications

Isolation of Leptospira spp. from a man living in a rural area of the Municipality of Cruz Alta, RS, Brazil

Isolation of Leptospira spp. from a man living in a rural area of the Municipality of Cruz Alta, RS, Brazil

Alteraciones histopatológicas en ratas naturalmente infectadas con Leptospira

Attenuated Nephritis in Inducible Nitric Oxide Synthase Knockout C57BL/6 Mice and Pulmonary Hemorrhage in CB17 SCID and Recombination Activating Gene 1 Knockout C57BL/6 Mice Infected with Leptospira interrogans

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