Rats with physically disconnected hypothalamo‐pituitary tracts no longer contain vasopressin‐oxytocin gene transcripts in the posterior pituitary lobe

Mohr, Evita; Zhou, An; Thorn, Niels A.; Richter, Dietmar

doi:10.1016/0014-5793(90)81407-f

Cited by 55 publications

(30 citation statements)

References 13 publications

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“…In the chorio-decidua, this difference is not likely due to differences in the coding region of the mRNA since both the 5'-and 3'-specific probes were completely protected by RNA from these tissues. The difference in size may be due to a variation in the poly(A) tail length, as has previously been noted in bovine hypothalamic and ovarian OT mRNA (30 Our results also demonstrate that OTmRNA the human pituitary and corpus luteum thus suy vious studies in mammals (35)(36)(37)(38) (39).…”

Section: Discussionsupporting

confidence: 72%

Synthesis of oxytocin in amnion, chorion, and decidua may influence the timing of human parturition.

Chibbar¹,

Miller²,

Mitchell³

1993

J. Clin. Invest.

245

116

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Despite the widespread clinical use of oxytocin (OT) as a potent and specific stimulant of labor, previous research data have not supported a role for OT in the physiology of normal human parturition. We have demonstrated synthesis of OT mRNA in amnion, chorion, and decidua using Northern blot analysis, ribonuclease protection assays, and in situ hybridization. Probes directed towards both the 3' and 5' ends of the gene have been used. Levels were highest in decidua with considerably less in chorion and amnion and very low levels in placenta. The transcript size in decidua appears to be 60-80 nucleotides smaller than the transcripts in amnion and chorion. OT gene expression in chorio-decidual tissues increased three-to fourfold around the time of labor onset. Estradiol stimulated synthesis of OT mRNA during in vitro incubation. These results support the hypothesis of a paracrine system involving OT and sex steroids within intrauterine tissues wherein significant changes could occur without being reflected in the maternal circulation. Such a paracrine system could rationalize a long-sought role for oxytocin in the physiology of human labor. These data may lead to novel approaches towards prevention or treatment of preterm labor. (J. Clin. Invest. 1993. 91:185-192.)

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Section: Discussionsupporting

confidence: 72%

Synthesis of oxytocin in amnion, chorion, and decidua may influence the timing of human parturition.

Chibbar¹,

Miller²,

Mitchell³

1993

J. Clin. Invest.

245

116

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“…However, biochemical and morphological studies have shown that nerve cells that constitute the rat hypothalamo-neurohypophyseal tract harbor various RNA species-for instance, arginine vasopressin (AVP) and oxytocin (OT) mRNAs (9)(10)(11) as well as BC1 RNA (12)-in the axonal domain. In the homozygous Brattleboro rat (BB rat), mutant AVP transcripts are not detectable in the axonal compartment (13) even though the cell bodies of magnocellular neurons contain this mRNA (14).…”

mentioning

confidence: 99%

Differential subcellular mRNA targeting: deletion of a single nucleotide prevents the transport to axons but not to dendrites of rat hypothalamic magnocellular neurons.

Mohr

Morris

Richter

1995

Proc. Natl. Acad. Sci. U.S.A.

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“…Interestingly, this is accompanied by an elongation of their poly(A) tails although the physiological significance remains to be determined [4][5][6]. Both AVP and OT mRNAs are subject to axonal targeting [7][8][9] and hyperosmolar stimuli give rise to a differential increase in the level of AVP (17-fold) and OT (3-fold) mRNAs in the axonal compartment [8,[10][11][12]. The axonal transcripts exhibit shorter poly(A) tracts than their counterparts in the cell bodies [2], and a poly(A) tail polymorphism following osmotic challenge is not observed for these transcripts [12].…”

Section: Introductionmentioning

confidence: 99%

Effect of hypoosmolality on the abundance, poly(A) tail length and axonal targeting of arginine vasopressin and oxytocin mRNAs in rat hypothalamic magnocellular neurons

et al. 1995

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Arginine vasopressin (AVP) and oxytocin (OT) mRNAs are targeted to the axonal compartment of rat hypothalamic magnocellular neurons. Salt-loading results in a considerable rise in hypothalamic and axonal AVP mRNA but only a moderate increase for axonal OT mRNA. Here we report that hypoosmolality gives rise to a rapid decrease of axonal AVP encoding transcripts to undetectable levels after 2 weeks. The levels of OT mRNA in the axonal compartment did not change significantly. In the hypothalamus the mRNA for AVP also decreased. The size of the poly(A) tract of AVP encoding transcripts appeared to be strictly correlated with plasma osmolality. In contrast, the amount and size of OT encoding mRNAs were only moderately or not influenced by hypoosmolar stimuli.infusion of the AVP V2-receptor agonist 1-deamino-[8-D-arginine] vasopressin (DDAVP) by use of subcutaneously implanted osmotic minipumps combined with a liquid diet to induce overhydration. In the hypothalamus hypoosmolality induced a reduction in both the abundance of AVP mRNA and the length of its poly(A) tract; the latter appears to be strictly correlated with plasma osmolality. In addition, a rapid and steady depletion of the axonal AVP mRNA pool was observed with undetectable levels after 2 weeks of overhydration. Surprisingly, the oxytocinergic system was considerably less affected by this treatment. Materials and methods

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Rats with physically disconnected hypothalamo‐pituitary tracts no longer contain vasopressin‐oxytocin gene transcripts in the posterior pituitary lobe

Cited by 55 publications

References 13 publications

Synthesis of oxytocin in amnion, chorion, and decidua may influence the timing of human parturition.

Synthesis of oxytocin in amnion, chorion, and decidua may influence the timing of human parturition.

Differential subcellular mRNA targeting: deletion of a single nucleotide prevents the transport to axons but not to dendrites of rat hypothalamic magnocellular neurons.

Effect of hypoosmolality on the abundance, poly(A) tail length and axonal targeting of arginine vasopressin and oxytocin mRNAs in rat hypothalamic magnocellular neurons

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