Ratiometric fluorescence monitoring of α-glucosidase activity based on oxidase-like property of MnO2 nanosheet and its application for inhibitor screening

Shi, Menglan; Yao, Chuang; Xu, Guanhong; Wei, Fangdi; Xu, Xiaoman; Cheng, Xin‐Bing; Chai, Yuying; Sohail, Muhammad; Hu, Qin

doi:10.1016/j.aca.2019.05.037

Cited by 47 publications

(12 citation statements)

References 30 publications

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“…Figure C shows that oxABTS had no significant absorption spectral change with the addition of AgNCs, revealing no interaction or complex formation between AgNCs and oxABTS and ruling out static quenching . Meanwhile, no fluorescence lifetime change of AgNCs was found after the oxABTS addition (Figure D), which suggested that no electron or energy transfer occurred between AgNCs and oxABTS . On the basis of the above results, the fluorescence quenching mechanism was attributed to the IFE process between AgNCs and oxABTS.…”

Section: Resultsmentioning

confidence: 84%

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Fabrication of Bioresource-Derived Porous Carbon-Supported Iron as an Efficient Oxidase Mimic for Dual-Channel Biosensing

et al. 2021

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Herein, we designed a new strategy for fabricating a renewable bioresource-derived N-doped hierarchical porous carbon-supported iron (Fe/NPC)-based oxidase mimic. The obtained results suggested that Fe/NPC possessed a large specific surface area (1144 m2/g) and pore volume (0.62 cm3/g) to afford extensive Fe-Nx active sites. Taking advantages of the remarkable oxidase-mimicking activity, outstanding stability, and reusability of Fe/NPC, a novel dual-channel biosensing system was strategically fabricated for sensitively determining acetylcholinesterase (AChE) through the integration of Fe/NPC and fluorescent silver nanoclusters (AgNCs) for the first time. The limits of detection for AChE can achieve as low as 0.0032 and 0.0073 U/L by the outputting fluorometric and colorimetric dual signals, respectively. Additionally, this dual-signal system was applied to analyze human erythrocyte AChE and its inhibitor with robust analytical performance. This work provides one sustainable and effective avenue to apply a bioresource for fabricating an Fe/NPC-based oxidase mimic with high catalytic performance and also gives new impetuses for developing novel biosensors by applying Fe/NPC-based enzyme mimics as substitutes for the natural enzyme.

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Section: Resultsmentioning

confidence: 84%

“…49 Meanwhile, no fluorescence lifetime change of AgNCs was found after the oxABTS addition (Figure 5D), which suggested that no electron or energy transfer occurred between AgNCs and oxABTS. 50 On the basis of the above results, the fluorescence quenching mechanism was attributed to the IFE process between AgNCs and oxABTS.…”

Section: ■ Experimental Sectionmentioning

confidence: 84%

Fabrication of Bioresource-Derived Porous Carbon-Supported Iron as an Efficient Oxidase Mimic for Dual-Channel Biosensing

et al. 2021

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“…However, the reductive targets will also cause interference problem. Therefore, the in situ formation of fluorescence species based on the OXD-like activity of MnO 2 nanosheets combined with the utilization of a specific chemical reaction for recognition of special molecules may be employed to develop a nanoplatform with good sensitivity and selectivity, which may be a promising work.…”

Section: Introductionmentioning

confidence: 99%

Universal Nanoplatform for Formaldehyde Detection Based on the Oxidase-Mimicking Activity of MnO₂ Nanosheets and the In Situ Catalysis-Produced Fluorescence Species

Zhao

Shen

Liu

et al. 2021

J. Agric. Food Chem.

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Formaldehyde (HCHO) pollution is a scientific problem of general concern and has aroused wide attention. In this work, a fluorometric method for sensitive detection of formaldehyde was developed based on the oxidase-mimicking activity of MnO2 nanosheets in the presence of o-phenylenediamine (OPD). The MnO2 nanosheets were prepared by the bottom-up approach using manganese salt as the precursor, followed by the exfoliation with bovine serum albumin. The as-prepared MnO2 nanosheets displayed excellent oxidase-mimicking activity, and can be used as the nanoplatform for sensing in fluorometric analysis. OPD was used as a typical substrate because MnO2 nanosheets can catalyze the oxidation of OPD to generate yellow 2,3-diaminophenazine (DAP), which can emit bright yellow fluorescence at the wavelength of 560 nm. While in the presence of formaldehyde, the fluorescence was greatly quenched because formaldehyde can react with OPD to form Schiff bases that decreased the oxidation reaction of OPD to DAP. The main mechanism and the selectivity of the platform were studied. As a result, formaldehyde can be sensitively detected in a wide linear range of 0.8–100 μM with the detection limit as low as 6.2 × 10–8 M. The platform can be used for the detection of formaldehyde in air, beer, and various food samples with good performance. This work not only expands the application of MnO2 nanosheets in fluorescence sensing, but also provides a sensitive and selective method for the detection of formaldehyde in various samples via a new mechanism.

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“…The above single-emission fluorescence sensors displayed limited visualization effects or sensitivity when compared with dual-emission fluorescence sensors. Some ratiometric fluorescence sensors were proposed based on the quenching ability or oxidase-like property of MnO 2 nanosheets. − For example, MnO 2 nanosheets oxidized o -phenylenediamine into 2,3-diaminophenazine with a strong fluorescence emission at 575 nm, which can quench Ag nanoclusters at 450 nm by the IFE, and based on this mechanism, AA was detected …”

Section: Introductionmentioning

confidence: 99%

CDs–MnO₂–TPPS Ternary System for Ratiometric Fluorescence Detection of Ascorbic Acid and Alkaline Phosphatase

2021

ACS Omega

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Manganese dioxide (MnO 2 ) nanosheet-based fluorescence sensors often use oxidase-like activity or wide absorption spectrum for detection of antioxidants. In those strategies, MnO 2 nanosheets were reduced to Mn 2+ by antioxidants. However, few strategies emphasize the role of Mn 2+ obtained from MnO 2 reduction in the design of the fluorescence sensor. Herein, we expanded the application of a MnO 2 nanosheet-based fluorescence sensor by involving Mn 2+ in the detection process using ascorbic acid (AA) as a model target. In this strategy, carbon dots (CDs), MnO 2 nanosheets, and tetraphenylporphyrin tetrasulfonic acid (TPPS) comprise a ternary system for ratiometric fluorescence detection of AA. Initially, CDs were quenched by MnO 2 nanosheets based on the inner filter effect, while TPPS maintained its fluorescence intensity. After the addition of AA, MnO 2 nanosheets were reduced to Mn 2+ so that the fluorescence intensity of CDs was recovered and TTPS was quenched by coordination with Mn 2+ . Overall, AA triggered an emission intensity increase at 440 nm for CDs and a decrease at 640 nm for TPPS. The ratio intensity of CDs to TPPS ( F 440 / F 640 ) showed a good linear relationship from 0.5 to 40 μM, with a low detection limit of 0.13 μM for AA detection. By means of the alkaline phosphatase (ALP)-triggered generation of AA, this strategy can be applied for the detection of ALP in the range of 0.1–100 mU/mL, with a detection limit of 0.04 mU/mL. Furthermore, this sensor was applied to detect AA and ALP in real, complex samples with ideal recovery. This novel platform extended the application of MnO 2 nanosheet-based fluorescence sensors.

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Ratiometric fluorescence monitoring of α-glucosidase activity based on oxidase-like property of MnO2 nanosheet and its application for inhibitor screening

Cited by 47 publications

References 30 publications

Fabrication of Bioresource-Derived Porous Carbon-Supported Iron as an Efficient Oxidase Mimic for Dual-Channel Biosensing

Fabrication of Bioresource-Derived Porous Carbon-Supported Iron as an Efficient Oxidase Mimic for Dual-Channel Biosensing

Universal Nanoplatform for Formaldehyde Detection Based on the Oxidase-Mimicking Activity of MnO₂ Nanosheets and the In Situ Catalysis-Produced Fluorescence Species

CDs–MnO₂–TPPS Ternary System for Ratiometric Fluorescence Detection of Ascorbic Acid and Alkaline Phosphatase

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Ratiometric fluorescence monitoring of α-glucosidase activity based on oxidase-like property of MnO2 nanosheet and its application for inhibitor screening

Cited by 47 publications

References 30 publications

Fabrication of Bioresource-Derived Porous Carbon-Supported Iron as an Efficient Oxidase Mimic for Dual-Channel Biosensing

Fabrication of Bioresource-Derived Porous Carbon-Supported Iron as an Efficient Oxidase Mimic for Dual-Channel Biosensing

Universal Nanoplatform for Formaldehyde Detection Based on the Oxidase-Mimicking Activity of MnO2 Nanosheets and the In Situ Catalysis-Produced Fluorescence Species

CDs–MnO2–TPPS Ternary System for Ratiometric Fluorescence Detection of Ascorbic Acid and Alkaline Phosphatase

Contact Info

Product

Resources

About

Universal Nanoplatform for Formaldehyde Detection Based on the Oxidase-Mimicking Activity of MnO₂ Nanosheets and the In Situ Catalysis-Produced Fluorescence Species

CDs–MnO₂–TPPS Ternary System for Ratiometric Fluorescence Detection of Ascorbic Acid and Alkaline Phosphatase