Ratiometric and turn-on monitoring for heavy and transition metal ions in aqueous solution with a fluorescent peptide sensor

Joshi, Bishnu P.; Park, Junwon; Lee, Wan In; Lee, Keun Hyeung

doi:10.1016/j.talanta.2008.12.062

Cited by 198 publications

(59 citation statements)

References 39 publications

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“…As a pre-organization of the ligand site of the peptides is required for the selective monitoring of metal ions, 3,5 all peptide sensors employed in this study commonly contained Pro-Gly amino acids to have pre-organized structure.…”

Section: Resultsmentioning

confidence: 99%

“…Furthermore, we synthesized several peptide sensors that had different secondary structures and found out that metal-binding affinity of the peptide sensors could be tuned by specific secondary structures. 4,5 In the present study, we investigated whether or not metalbinding affinity of the small peptide sensors could be tuned by modulating amino acid sequence. To investigate the role of amino acids for selective monitoring of Zn 2+ ions, we chose Cp1 (-CPGHE) as a model peptide and synthesized several peptide sensors, as shown in Table 1.…”

Section: Introductionmentioning

confidence: 99%

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Design and Synthesis of Metallopeptide Sensors: Tuning Selectivity with Ligand Variation

Kim¹,

Joshi²,

Lee

2010

Bulletin of the Korean Chemical Society

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We chose a fluorescent pentapeptide sensor (-CPGHE) containing a dansyl fluorophore as a model peptide and investigated whether the selectivity and sensitivity of the peptides for heavy and transition metal ions could be tuned by changing amino acid sequence. In this process, we developed a selective peptide sensor, Cp1-d (-HHPGE, Kd = 670 nM) for detection of Zn 2+ in 100% aqueous solution and a selective and sensitive peptide sensor, Cp1-e (-CCHPGE, Kd = 24 nM) for detection of Cd 2+ in 100% aqueous solution. Overall results indicate that the selectivity and sensitivity of the metallopeptide sensors to specific heavy and transition metal ions can be tuned by changing amino acid sequence.Key Words: Peptides, Zn Cp1-a Where Dansyl=Cp1-e Scheme 1. Chemical structures of representative peptide sensors

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Design and Synthesis of Metallopeptide Sensors: Tuning Selectivity with Ligand Variation

Kim¹,

Joshi²,

Lee

2010

Bulletin of the Korean Chemical Society

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“…(n = 9, R = 0.99794, SD = 7.47064). The detection limit was determined from the following equation [21,22]: detection limit = 3 9 SD/B, where SD is the standard deviation of blank measurement and B is the slope of the fluorescence intensity versus the Ni 2? concentration.…”

Section: Binding Constants and Detection Limitmentioning

confidence: 99%

A highly selective chemosensor for nickel(II) based on fluorescence quenching of a bispyrazole derivative

Yan

et al. 2016

Res Chem Intermed

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A novel bispyrazole, 2,6-bis(5-phenyl-1H-pyrazol-3-yl)-pyridine, was designed and synthesized. The bispyrazole showed extreme selectivity for Ni 2? over other metal ions such as ? in MeOH-H 2 O solution. The sensing sensitivity and binding properties of the bispyrazole for nickel(II) ion were investigated by fluorescent spectroscopic techniques. It was found that the bispyrazole possessed high selectivity and sensitivity toward Ni 2? over other metal ions. The binding stoichiometry was confirmed as 1:1 (bispyrazole/Ni 2? ) by Job plot experiment and the linearity of the Benesi-Hildebrand plot. The binding constants K and thermodynamic parameters (DH, DG, and DS) at different temperatures were obtained. Meanwhile, the detection limit of the bispyrazole for Ni 2? was determined to be 3.25 9 10 -7 mol L -1 .

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“…Fluorescent peptide sensors have also been designed successfully for the detection of various metal ions [18][19][20]. Because peptides do not usually have fluorescent properties to be essential for sensing, a fluorophore group needs to be conjugated to them for developing fluorescent peptide sensors.…”

Section: +mentioning

confidence: 99%

Selective Detection of Mercury Ion by a Fluorescent Sensor Based on Dipeptide Containing Cysteine

Azuma

Fukushima

2014

J. Photopol. Sci. Technol.

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Two fluorescent peptidyl chemosensors containing cysteine and a dansyl group (DNS-γGlu-Cys, DNS-Cys-Glu) were prepared via Boc solution phase peptide synthesis for monitoring heavy metal ions on the basis of phytochelatin peptide sequence. DNS-Cys-Glu displayed a selective fluorescence turn-off response to Hg 2+ over other heavy metal ions. In contrast, the addition of various metal ions to DNS-γGlu-Cys resulted in no significant fluorescent changes. For DNS-Cys-Glu, because the thiol group of Cys is adjacent to the sulfonamide group, they can bind to Hg 2+ by cooperative electrostatic interactions. As a result, the quenching of the fluorescence intensity can be explained in terms of effective electron transfer from the excited dansyl fluorophore to the bound Hg 2+ . DNS-Cys-Glu could be applicable to homogeneous Hg 2+ detection.

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Ratiometric and turn-on monitoring for heavy and transition metal ions in aqueous solution with a fluorescent peptide sensor

Cited by 198 publications

References 39 publications

Design and Synthesis of Metallopeptide Sensors: Tuning Selectivity with Ligand Variation

Design and Synthesis of Metallopeptide Sensors: Tuning Selectivity with Ligand Variation

A highly selective chemosensor for nickel(II) based on fluorescence quenching of a bispyrazole derivative

Selective Detection of Mercury Ion by a Fluorescent Sensor Based on Dipeptide Containing Cysteine

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