Rate of Increase of Antiboby Affinity in Different Rat Strains

Lamelin, J P; Paul, W E

doi:10.1159/000230418

Cited by 13 publications

(5 citation statements)

References 7 publications

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“…Similar results were obtained in investigating the response t o DNP-Fla. N o change in the affinity of antibody produced during the IgM response was detected, whereas the IgC anti-DNP response t o 50 pg per mouse showed an increase in affinity of about 10-fold during the period studied. These affinity changes in the IgG response are analogous t o those found in rabbits [ 1, 31 and in the rat [22]. A striking feature of this study has been that, in every mouse, at every time point, the relative affinity of IgM PFC was lower than that of IgG antibody plaque formers present in the same cell pool.…”

Section: Discussionsupporting

confidence: 82%

“…Despite the different nature of these two antigens, no significant difference in the sequence of affinity changes (time course and antigen dependence) was found. The similarity of the affinity results using the plaque inhibition technique in mice and previously published data [ 1,18,22,32,331 further suggests the validity of the method employed.…”

Section: Discussionsupporting

confidence: 78%

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Studies on antibody affinity in mice

Huchet

Feldmann

1973

Eur J Immunol

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The plaque inhibition method of measuring the affinity of single antibody‐forming cells (AFC) developed by Andersson has been modified for use with haptens. Using this method, it was possible to make simultaneous determinations of the relative affinities of IgG and IgM plaques during the primary and secondary responses. It was shown that there was no maturation of the affinity of antibody‐released IgM AFC in the primary response in mice. There was an increase in affinity of the antibody released from IgG AFC, which was antigen dose‐ and time‐dependent, confirming results obtained by others. Furthermore, at any time during the course of the primary response, the affinity of the IgM antibody released by AFC was always lower than that of IgG plaque‐forming cells. This difference averaged about 10‐fold. The same factors were shown to influence the affinity changes of IgG AFC observed during the secondary response. In addition the booster, or secondary dose of antigen used, was important. Highest affinity antibody was produced in response to intermediate antigen doses. The maturation of affinity affects the generation of memory cells to the same degree as the antibody‐secreting cells during the primary response, since similar affinity changes occurred in the absence of detectable primary responses. Finally, when the period between primary stimulation and challenge is 5 months, there is a decrease of between 2 and 2.6 log10 in the concentrations of DNP lysine needed to inhibit 50 % of the IgG antibody‐forming cells depending on the doses used for priming. The variation of affinity of IgM antibody was much less obvious, with a 6‐fold difference at the maximum, which was independent of the doses of antigen used for priming or boosting, and of the time elapsed between the two antigenic stimuli.

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Section: Discussionsupporting

confidence: 82%

Section: Discussionsupporting

confidence: 78%

Studies on antibody affinity in mice

Huchet

Feldmann

1973

Eur J Immunol

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“…In particular, antibodies specific for 'link' determinants are relatively more abundant early in the course of some immune responses (Borek & Silverstein 1965, Henney 1970 than are hapten specific antibodies. On the other hand, the specificity characteristics of cells mediating DNA synthetic reactions have been shown not to change significantly in the course of immune responses to DNP-proteins (Paul et al 1968, Lamelin & Paul 1970.…”

Section: Mechanisms Controlling Differences In Apparent Receptor mentioning

confidence: 99%

Functional Specificity of Antigen‐Binding Receptors of Lymphocytes

Paul

1970

Immunological Reviews

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“…The adult antibody response is generally characterized by a marked degree of heterogeneity of affinity of the antibody for the antigenic determinant (1,2). With time after immunization there appears to be a preferential selection of high affinity antibody-producing B lymphocytes resulting in an increase in the average affinity of the serum antibody (2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19). It is usually assumed that individual B lymphocytes produce a homogeneous antibody product (20) and that the heterogeneity of serum antibody is a consequence of the stimulation, by antigen, of a number of different B lymphocytes (18).…”

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confidence: 99%

Ontogeny of B-lymphocyte function. II. Ability of endotoxin to increase the heterogeneity of affinity of the immune response of B lymphocytes from fetal mice.

Goidl¹,

Klass²,

Siskind³

1976

The Journal of Experimental Medicine

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We (1) have previously demonstrated that B lymphocytes from neonatal or late fetal mice produced an immune response which was highly restricted with respect to heterogeneity of avidity of the plaque-forming cells (PFC) 1 as compared with the PFC response produced by B lymphocytes from adult donors. These studies were carried out by transferring the lymphoid population to be tested, together with excess adult thymus cells, into lethally irradiated, syngeneic mice. Under these conditions the functional capacity of the B lymphocytes from immature animals could be assayed in an adult environment in the presence of excess adult T lymphocytes. Thus, the restricted heterogeneity of the immune response observed with B lymphocytes from neonatal or 17-day fetal donors could be ascribed to a functional immaturity of the B lymphocyte itself. When evaluated in this system, it was found that B lymphocytes acquire adult functional capabilities between 7 and 10 days of age in LAF1 mice. Our studies described above (1), together with work from a number of other laboratories (2-17), have thus demonstrated an ordered development of various functional and morphological characteristics of the immune system. It was suggested (1) that the temporally abrupt maturation of the competence of B lymphocytes to generate an adult-type, heterogeneous, immune response implied a differentiation event rather than the accumulation of somatic mutations. If this hypothesis were correct, then the B-cell population present in a neonatal animal should have the genetic information required to synthesize the same heterogeneous population of antibody molecules as the adult is capable of producing. This prediction was tested in the present paper by determining whether neonatal or fetal B lymphocytes could be induced, in the transfer system described above, to produce a heterogeneous antibody response if given

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Rate of Increase of Antiboby Affinity in Different Rat Strains

Cited by 13 publications

References 7 publications

Studies on antibody affinity in mice

Studies on antibody affinity in mice

Functional Specificity of Antigen‐Binding Receptors of Lymphocytes

Ontogeny of B-lymphocyte function. II. Ability of endotoxin to increase the heterogeneity of affinity of the immune response of B lymphocytes from fetal mice.

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