Functional Specificity of Antigen‐Binding Receptors of Lymphocytes

Paul, William E.

doi:10.1111/j.1600-065x.1970.tb00359.x

Cited by 30 publications

(33 citation statements)

References 120 publications

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“…Some studies suggest that T cells can recognize different antigenic structures than do B cells (23), although frequently the distinction between antigen-binding cells and cell stimulation has not been made. In our experiments, the proportion of T and B cells in populations fractionated with respect to several hapten or protein antigens was essentially the same, and comparable to values obtained with unfractionated spleen cell populations.…”

Section: Resultsmentioning

confidence: 99%

Binding of Thymus- and Bone Marrow-Derived Lymphoid Cells to Antigen-Derivatized Fibers

Rutishauser

Edelman

1972

Proc. Natl. Acad. Sci. U.S.A.

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Thymus-derived lymphocytes (T cells) and bone marrow-derived lymphocytes (B cells) from mouse spleens bind specifically to antigen-derivatized nylon fibers. The fiber-bound population consisted of about 60%-70% B cells and 30% T cells as determined by cytotoxicity, fluorescence, and antibody-complement binding assays. Essentially all fiber-bound cells were viable and could be accounted for as T or B cells. Enriched populations of T or B cells could be isolated on the fibers by destruction of one or the other cell type with the appropriate antiserum plus complement. T or B cells could also be fractionated according to their relative affinity (or avidity) for a given antigen.In previous reports, we have described the fractionation of specific antigen-binding cells from lymphoid cell populations of immunized and unimmunized animals by specific attachment to antigen-derivatized fibers (1, 2). Although the isolated lymphoid cells all have specificity for the chosen antigens, it is likely that they are heterogeneous in their function and degree of differentiation. The most conspicuous functional heterogeneity among lymphocytes is reflected by the presence in the population of thymus-derived lymphocytes (T cells) and bone marrow-or bursa-derived lymphocytes (B cells) (3). Additional distinctions must be made between thymocytes and competent T cells. Similarly, B cells include several functional classes, notably antigen-sensitive B cells that can differentiate into secreting cells when triggered by antigen, and the secreting cells or plaque-forming cells themselves.Out studies (1, 2) have indicated that specific antigenbinding cells isolated by fiber fractionation do not include either thymocytes or plaque-forming cells. In the present paper, we provide evidence that antigen-derivatized fibers bind both T and B cells. The cells can be fractionated according to their relative avidities* for antigen and, by the use of appropriate antisera, enriched populations of T or B cells with specificity for a given antigenic determinant can be obtained.

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Section: Resultsmentioning

confidence: 99%

Binding of Thymus- and Bone Marrow-Derived Lymphoid Cells to Antigen-Derivatized Fibers

Rutishauser

Edelman

1972

Proc. Natl. Acad. Sci. U.S.A.

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“…The other cell fails to secrete detectable amounts of antibody, but nevertheless plays a role in the activation of the precursor described above (3,20,21). In the mouse, this "helper" cell has been demonstrated to be thymus-derived and to bear the isoantigen 0 (8,22).…”

Section: Discussionmentioning

confidence: 99%

Carrier Function in Anti-Hapten Antibody Responses

Katz

Paul

Goidl

et al. 1971

The Journal of Experimental Medicine

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198

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The studies reported here demonstrate that immunocompetent lymphoid cells from allogeneic donor guinea pigs stimulate the synthesis of anti-DNP and anti-OVA antibodies by recipients previously primed with DNP-OVA. This allogeneic effect occurs spontaneously in the absence of any further anti-genic challenge. Furthermore, the transfer of allogeneic cells prepares DNP-OVA-primed recipients for a striking secondary anti-DNP response to DNP-BGG; this occurs in equal degree whether or not the cells are derived from BGG-primed donors. We suggest that the allogeneic cells function by virtue of a specific immunologic attack of grafted cells on host cells. This conclusion is made on the basis of the following evidence: (a) The failure of observing the phenomenon with L2C leukemia cells and irradiated strain 2 lymph node and spleen cells which, although capable of initiating a host-versus-graft response, are incapable of mediating graft-versus-host reactions; and (b) the inability of (strain 2 x strain 13) F1 hybrids to mediate the allogeneic effect in strain 13 recipients. The analysis of this phenomenon may offer a key to the delineation of mechanisms involved in the activation of precursors of antibody-forming cells.

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“…By hypothesis, the specific combination of antigen with its receptor(s) results in activation of immune differentiation and subsequent synthesis of antibody, although under certain conditions specific inactivation and tolerance can ensue (1,2). Antigen receptors located on the surface of certain lymphoid cells (3)(4)(5)(6)(7)(8)(9) are considered to be representative of the eventual cell product with regard to both specificity and immunoglobulin class (10)(11)(12)(13). Therefore, the use of antibody directed against a particular class of heavy chain determinants displayed by antigen receptors could conceivably lead to the selective inhibition of the production of that immunoglobulin class resulting in an experimental dysgammaglobulinemia.…”

Section: (From the Department Of Immunology Mayo Medical School Rocmentioning

confidence: 99%

PRODUCTION OF a RUNTING SYNDROME AND SELECTIVE ΓA DEFICIENCY IN MICE BY THE ADMINISTRATION OF ANTI-HEAVY CHAIN ANTISERA

Murgita¹,

Mattioli²,

Tomasi³

1973

The Journal of Experimental Medicine

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Conventional Swiss mice were treated from birth with intraperitoneal injections of anti-immunoglobulins in an attempt to create an experimental dysgammaglobulinemia. Mice treated with anti-γM were immunologically suppressed in all immunoglobulin classes as determined by serum antibody titers, splenic plaque-forming cells, serum immunoglobulin levels, and immunofluorescent analysis of plasma cells in lymphoid tissues. Treatment immediately after birth with high concentrations of anti-γM leads to a developmental arrest characterized by severe immunosuppression, failure to gain weight, and premature death. The pathogenesis of anti-γM runting syndrome is unknown. Animals similarly treated with anti-γG, anti-γA, or control normal goat or rabbit γ-globulins developed normally. The frequency of occurrence and severity of anti-γM-induced runting syndrome is dependent upon the concentration of anti-γM-globulin administered. Administration of anti-γA resulted in a selective γA deficiency that was characterized by a marked reduction in serum-γA and an absence of γA-containing cells in the spleen. However, essentially normal numbers of plasma cells were found in the gastrointestinal lamina propria of anti-γA-treated animals concomitantly with suppressed levels of γA in intestinal fluids.

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Functional Specificity of Antigen‐Binding Receptors of Lymphocytes

Cited by 30 publications

References 120 publications

Binding of Thymus- and Bone Marrow-Derived Lymphoid Cells to Antigen-Derivatized Fibers

Binding of Thymus- and Bone Marrow-Derived Lymphoid Cells to Antigen-Derivatized Fibers

Carrier Function in Anti-Hapten Antibody Responses

PRODUCTION OF a RUNTING SYNDROME AND SELECTIVE ΓA DEFICIENCY IN MICE BY THE ADMINISTRATION OF ANTI-HEAVY CHAIN ANTISERA

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