Rat tissue reaction and cytokine production induced by antimicrobial photodynamic therapy

Abstract

Background: The antimicrobial photodynamic therapy (aPDT) inactivates the target cell via reactions among the photosensitizer (PS), Laser or Led and O 2 . The aim of this study was to evaluate the tissue reaction and cytokine production promoted by aPDT with curcumin photosensitizer. Methods: Polyethylene tubes containing saline solution (control), 5% sodium hypochlorite (NaOCl) and aPDT with curcumin PS 500 mg/L, were implanted into dorsal connective tissue of Wistar rats. After 7, 15, 30, 60 and 90 days of implantation, the animals were euthanized and the tubes with surrounding tissues were removed. The specimens were divided in two part, one half was processed, fixed and prepared for histological analysis by staining with hematoxylin and eosin. The other half was collected for IL-1β and IL-6 cytokine production using ELISA assay. The results were statistically analyzed by Kruskal-Wallis test followed by Dunn test (p < 0.05) for tissue reaction and ANOVA followed by Bonferroni's correction (p < 0.05) for ELISA. Results: All groups showed severe tissue reactions at 7 days, whilst a significantly decrease by time was observed. Regarding to cytokine production, aPDT increases the IL-1β levels in all periods of time (p < 0.05). However, for IL-6 levels, the highest value was observed with aPDT on the 90th day (p < 0.05). Conclusions: aPDT with curcumin PS 500 mg/L demonstrated biocompatibility similar to saline solution and induced the IL-1β and IL-6 cytokines production.

Antimicrobial Photodynamic Therapy: An Effective Alternative Approach to Control Bacterial Infections

“…IL-6 levels were highest at a 90-day period with aPDT. 41 Jung et al investigated the effect of PDT on Hemophilus influenza in vivo, and Streptococcus pneumoniae in Meriones unguiculatus rats. Twenty microliters of the bacterial solution (10 6 CFU) injected into the bullae under sterile conditions.…”

Effect of photodynamic therapy on the mechanical properties and bond strength of glass-fiber posts to endodontically treated intraradicular dentin

“…Helicoidal movements were performed in the apicocervical direction with the optical fiber to ensure uniform diffusion of light throughout the root canal. 18,19 The movements were repeated approximately 10 times/min. 20 The choice of light wavelength is directly related to the absorption characteristics of the PS used.…”

Terapia fotodinâmica antimicrobiana: avaliação in vitro e in vivo do efeito de diferentes fotossensibilizadores na inativação da endotoxina bacteriana

“…De fato, seu uso está fortemente relacionado às suas propriedades anti-inflamatórias e têm cada vez mais ganhado espaço na literatura, não se restringindo somente à área da Odontologia, demonstrando alta capacidade de inibir a produção de mediadores inflamatórios como TNF-α e IL-6 em modelos induzidos com LPS (Sun et al, 2017;Cheng et al, 2018;Curylofo-Zotti et al, 2018;Kumari et al, 2018;Lin et al, 2018;Lu et al, 2018;Sorrenti et al, 2018;Xiao et al, 2018;Zhang et al, 2019;Yan et al, 2019;Toraya et al, 2020). Nas doenças inflamatórias bucais, especialmente doença periodontal e periodontite apical, estas citocinas desempenham papel importante, modulando células da resposta imune inata (Silva et al, 2007;Salminen et al, 2014;Sivieri-Araujo et al, 2017;Curylofo-Zotti et al, 2018;Xiao et al, 2018). Nossos experimentos de cultura de células e choque séptico corroboram fortemente com estes estudos, indicando o poder desta molécula em interferir nas vias de sinalização das células, incluindo a inativação do fator de transcrição NF-κB (Li et al, 2017), mesmo em baixas concentrações.…”