Rat Passive Peritoneal Anaphylaxis Following Sensitisation with Guinea Pig Antiserum: an Immediate Hypersensitivity Reaction without Histamine Release

Abstract

Antigen challenge of rats, sensitised by an intraperitoneal injection of guinea pig antiserum, produced an antibody-dependent increase in concentrations, in the peritoneal fluids, of slow-reacting substance of anaphylaxis (SRS-A) and of extravasated, dye-labelled, plasma proteins but not of histamine or 5-hydroxytryptamine. The SRS-A released may not be the main mediator of the extravasation since pre-treatment of the rats with the SRS-A antagonists FPL 55712 and BRL 19880 had no effect on extravasation, and a number of compounds, given in a similar way, were more potent as inhibitors of SRS-A release than of extravasation. Rat passive peritoneal anaphylaxis (PPA), following sensitisation with guinea pig antiserum, provides an example of an immediate hypersensitivity reaction in which there is no antibody-dependent release of histamine and in which the SRS-A released may not mediate all of the extravasation produced.

A New Method of Testing Anti-Allergic Drugs

“…Never theless, new models were recently elaborated [2,3,14,15], evidently because of the lack of a quick for meth od providing adequate results in respect of the phar macologic reactivity of the immediate hypersensitivi ty reactions. Here we describe an easily performed as say which seems to be suitable for large-scale screen ing of anti-allergic drugs.…”