A New Method of Testing Anti-Allergic Drugs

Abstract

Rats were sensitized with a single intraperitoneal dose of bovine serum albumin in alhydrogel plus Bordetella pertussis vaccine, and local anaphylaxis was elicited in the paw by soluble antigen 2 weeks later. The response was mainly due to IgE-type antibodies and proved to be highly sensitive to beta-adrenoceptor agonists. Dexamethasone inhibited the response after a lag phase. Methysergide, disodium chromoglycate, diethylcarbamazine, BW 775/c, nordihydroguajaretic acid, and FPL 55712 were also suppressive, while mepyramine was without effect. A synergism between methysergide and FPL 55712 was shown. Active local paw anaphylaxis appears to be adequate and easily applicable for large-scale screening of anti-allergic drugs.

Anti-histaminic, anti-inflammatory and bronchorelaxant activities of 2, 7-dimethyl-3-nitro-4H pyrido [1,2-a] pyrimidine-4-one

“…It involves IgE antibodies and also biogenic amines [24,25]. Active paw anaphylaxis model has widely been utilized for screening of putative anti-allergic drugs [26]. A suppressive effect of ketotifen, an H1 antagonist [27].…”

Glucocorticoids in myocardial and cerebral infarction

“…Lipocortin fractions obtained from sephadex G-50 gels inhibited paw edema induced by bradykinin and carrageenin when given i.v. into CFLP mice and also reduced anaphylactic paw swelling in sensitized rats [69] when injected intrapedally along with the specific antigen. These results indicate that our lipocortin preparation had not only anti-PLA2 but also anti-inflammatory and anti-anaphylactic effects (unpublished observations).…”

Passive Air-Pouch Anaphylaxis in Rats

“…While PCA can be elicited in many animal species, rat homologous PCA has been widely used [2][3][4][5][6]. In addition to antiana phylactic agents, several types of agents can inhibit PCA [7][8][9]. In order to obtain information about the mode of action of agents effective in PCA tests, it is necessary to measure chemical mediators re leased in anaphylaxis.…”