Rat ovary glucocorticoid receptor: Identification and characterization

Schreiber, James R.; Nakamura, Kevin; Erickson, Gregory F.

doi:10.1016/0039-128x(82)90057-5

Cited by 96 publications

(35 citation statements)

References 20 publications

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“…The expression of glucocorticoid receptors in rat ovarian cells (Schreiber et al 1982) and the detection of cortisol binding globulin in FF (Mahajan et al 1980) supports the possibility of direct glucocorticoid action in the ovary. Glucocorticoid hormone metabolism by ovarian cells is also indicated by the expression of 11 -hydroxysteroid dehydrogenase mRNA (Tetsuka et al 1997) and enzyme activity in human granulosa cells (Michael & Cooke 1994).…”

Section: Introductionmentioning

confidence: 57%

“…High cortisol concentrations were measured in FF following ACTH treatment. Receptors for cortisol have been identified in rat granulosa cells (Schreiber et al 1982), and previous studies report glucocorticoid-mediated suppression of IGF-I synthesis in a number of different cell or tissue types in culture. Exposure to cortisol has been shown to reduce IGF-I levels in the culture medium of liver cells (Binoux et al 1982), and to attenuate IGF-I mRNA expression as well as IGF-I protein secretion by rat osteoblast and fibroblast cultures (McCarthy et al 1990).…”

Section: Figurementioning

confidence: 97%

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ACTH treatment disrupts ovarian IGF-I and steroid hormone production

Viveiros

Liptrap²

2000

Journal of Endocrinology

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Hyper-adrenal activity and increased glucocorticoid hormone release are associated with disruptions in reproductive function and adverse effects on the ovary. The aim of this investigation was to determine whether elevated glucocorticoid hormone levels can influence ovarian IGF-I synthesis and action in vivo. To elevate endogenous glucocorticoid levels, gilts were treated with ACTH during the luteal phase of the oestrous cycle (days 9-13) while the control group received saline. The gilts were subsequently ovariectomized on either day 14 or day 18 of the oestrous cycle. Follicular fluid (FF) was collected from individual follicles; IGF-I and steroid hormone concentrations were determined by radioimmunoassay, and IGF-binding protein (IGFBP) expression was assessed by Western ligand blotting. Granulosa cells were also recovered and placed in culture to determine IGF-I, progesterone (P 4 ) and oestradiol-17 (E 2 ) production levels. The cells were cultured in serum-free medium for 5 days and supplemented with: (a) media alone, (b) IGF-I, (c) FSH and androstenedione (A 4 ), or (d) IGF-I with FSH and A 4 . The FF from ACTH-treated gilts was characterized by elevated (P<0·05) cortisol levels on day 14 and lower (P<0·05) E 2 values on both day 14 and day 18. Lower (P<0·05) IGF-I concentrations were also measured in the FF of ACTH-treated gilts collected on day 18. This altered hormone profile in FF was associated with impaired IGF-I and steroid hormone synthesis by granulosa cells. IGFstimulated P 4 production (P<0·01) by cells recovered from ACTH-treated gilts on day 14 was lower (P<0·05). By day 18, IGF-I, P 4 and E 2 production by cells from the ACTH group were all significantly (P<0·05) lower. These results demonstrate that increased glucocorticoid concentrations can disrupt subsequent ovarian IGF-I synthesis and IGF action in vivo and can, potentially, impair follicle maturation.

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Section: Introductionmentioning

confidence: 57%

Section: Figurementioning

confidence: 97%

ACTH treatment disrupts ovarian IGF-I and steroid hormone production

Viveiros

Liptrap²

2000

Journal of Endocrinology

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“…A further increase in the concentration by a factor 10 to 100 ng prednisolone/ml led to a further increase of a factor of 3.1 for oestradiol and 3.8 for progesterone; however, these values are lower than during interval II, which sug gests that prednisolone is not merely a precursor of ste roid synthesis; it is presumed that it acts directly on the enzymatic synthesis as it has been proved for the aromatase activity on the enzymatic synthesis as it has been proved for the aromatase activity of adipose tissue [1]. Glucocorticoid receptors in the ovaries have been de scribed [9], as has the existence of corticoid-binding globulin in the follicular fluid [10].…”

Section: Discussionmentioning

confidence: 99%

Prednisolone Promotes the Secretion of Progesterone and Oestradiol by Luteinised Granulosa Cells Independent of Human Chorionic Gonadotropin

Würfel¹,

Steck²,

Hertwig³

et al. 1990

Gynecol Obstet Invest

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An in vitro model based on luteinised granulosa cells gained during an in vitro fertilisation programme examines the question of whether prednisolone, and thus glucocorticoids in general, are capable of exerting an influence on the secretion of oestradiol and progesterone. It can be demonstrated that prednisolone leads to dosage-dependent increases in the concentration of both steroids, even independently of concurrent stimulation by human chorionic gonadotropin. A similar mechanism for aromatase activity of human adipose cells is suggested.

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“…However, it is not known whether a local effect of corticosteroids "on the ovaries is involved in the expression of their function. Different glucocorticoid levels have been shown to act on gonadotropins and to influence LH, FSH, Ez, and progesterone (P) levels in humans and animals (14)(15)(16). For example, high levels of corticosteroids can inhibit FSH-induced E2 production by GC.…”

Section: Resultsmentioning

confidence: 99%