Rat full term amniotic fluid harbors highly potent stem cells

Mun-Fun, Hoo; Ferdaos, Nurfarhana; Hamzah, Siti Nurusaadah; Ridzuan, Noridzzaida; Hisham, Nurul Afiqah; Abdullah, Saifollah; Ramasamy, Rajesh; Cheah, Pike See; Karrupiah, Thilakavathy; Yazid, Mohd Nazri; Nordin, Norshariza

doi:10.1016/j.rvsc.2015.07.010

Cited by 14 publications

(15 citation statements)

References 63 publications

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“…Our results were also supported by the study conducted by De Coppi and co-workers on the ability of rat AFSC to differentiate into the three primary germ layers: (1) mesoderm; (2) endoderm; and (3) ectoderm [71,90] Both 46C and R3 can be directly induced to differentiate into neural lineage through the (1) formation of multicellular aggregates, embryoid bodies, EBs, by 4-/4+ protocol [69], with the addition of all-trans retinoic acid (ATRA) as the neural inducer and also via (2) monoculture adherence protocol [93]. The abilities of mESCs and rat AFSCs to differentiate into functional neuronal and neuronal supporting cell subtypes have been reported previously [69,71]. These discoveries were in good agreement with our study with 46C and R3 (Figure 1 and 2).…”

Section: Prospective Stem Cell Lines As An In Vitro Nd Modelsupporting

confidence: 89%

“…Two major populations of stem cells were found in amniotic fluids as reported by Cananzi and co-workers, namely; (1) amniotic fluid mesenchymal stem cells and amniotic fluid stem cells (AFSC) [92]. We have successfully isolated and characterised AFSC in rats [71]. We categorised these cells as broadly multipotent stem cell based on the presence of the surface antigen, c-kit (CD117, a type III tyrosine kinase receptor of stem cell), which distinguishes it from mesenchymal stem cells [71].…”

Section: Prospective Stem Cell Lines As An In Vitro Nd Modelmentioning

confidence: 78%

“…Currently, research on stem cells derived from amniotic fluid have been extensively studied. AFSCs can be obtained during the second and third trimester of pregnancy or directly after birth in human; and in the second or third week of pregnancy in mice and rats [71,90,91]. Two major populations of stem cells were found in amniotic fluids as reported by Cananzi and co-workers, namely; (1) amniotic fluid mesenchymal stem cells and amniotic fluid stem cells (AFSC) [92].…”

Section: Prospective Stem Cell Lines As An In Vitro Nd Modelmentioning

confidence: 89%

“…Meanwhile, R3 is our in-house established AFSCs generated from rat full-term amniotic fluid. Both 46C and R3 have been shown to have the ability to differentiate into neurons [69][70][71][72].…”

Section: Prospective Stem Cell Lines As An In Vitro Nd Modelmentioning

confidence: 99%

“…We have successfully isolated and characterised AFSC in rats [71]. We categorised these cells as broadly multipotent stem cell based on the presence of the surface antigen, c-kit (CD117, a type III tyrosine kinase receptor of stem cell), which distinguishes it from mesenchymal stem cells [71]. The cells were observed to express Oct4 and Nanog.…”

Section: Prospective Stem Cell Lines As An In Vitro Nd Modelmentioning

confidence: 99%

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Prospective stem cell lines as in vitro neurodegenerative disease models for natural product research

et al. 2019

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The use of in vitro model for screening pharmacological compounds or natural products has gained global interest. The choice of cells to be manipulated plays a vital role in coming up with the best-suited model for specific diseases, including neurodegenerative diseases (ND). A good in vitro ND model should provide appropriate morphological and molecular features that mimic ND conditions where it can be used to screen potential properties of natural products in addition to unravelling the molecular mechanisms of ND. In this mini review, we intend to demonstrate two prospective stem cell lines as the potential cell source for in vitro ND model and compare them to the commonly used cells. The common source of cells that have been used as the in vitro ND models is discussed before going into details talking about the two prospective stem cell lines.

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Section: Prospective Stem Cell Lines As An In Vitro Nd Modelsupporting

confidence: 89%

Section: Prospective Stem Cell Lines As An In Vitro Nd Modelmentioning

confidence: 78%

Section: Prospective Stem Cell Lines As An In Vitro Nd Modelmentioning

confidence: 89%

Section: Prospective Stem Cell Lines As An In Vitro Nd Modelmentioning

confidence: 99%

Section: Prospective Stem Cell Lines As An In Vitro Nd Modelmentioning

confidence: 99%

See 3 more Smart Citations

Prospective stem cell lines as in vitro neurodegenerative disease models for natural product research

et al. 2019

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Accelerated wound closure: Systematic evaluation of cellulose acetate effects on biologically active molecules release from amniotic fluid stem cells

Nuge

Liu

Tshai

et al. 2021

Biotech and App Biochem

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Despite a lot of intensive research on cell-scaffold interaction, the focus is mainly on the capacity of construct scaffolds to regulate cell mobility, migration, and cytotoxicity. The effect of the scaffold's topographical and material properties on the expression of biologically active compounds from stem cells is not well understood. In this study, the influence of cellulose acetate (CA) on the electrospinnability of gelatin and the roles of gelatin-cellulose acetate (Ge-CA) on modulating the release of biologically active compounds from amniotic fluid stem cells (AFSCs) is emphasized. It was found that the presence of a small amount of CA could provide a better microenvironment that mimics AFSCs' niche. However, a large amount of CA exhibited no significant effect on AFSCs migration and infiltration. Further study on the effect of surface topography and mechanical properties on AFSCs showed that the tailored microenvironment provided by the Ge-CA scaffolds had transduced physical cues to biomolecules released into the culture media. It was found that the AFSCs seeded on electrospun scaffolds with less CA proportions have profound effects on the secretion of metabolic compounds compared to those with higher CA contained and gelatin coating.The enhanced secretion of biologically active molecules by the AFSCs on the electrospun scaffolds was proven by the accelerated wound closure on the injured human dermal fibroblast (HDF) model. The rapid HDF cell migration could be anticipated due to a higher level of paracrine factors in AFSCs media. Our study demonstrates that the fibrous topography and mechanical properties of the scaffold are a key material property that modulates the high expression of

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miR-351-3p Promotes Rat Amniotic Fluid-Derived Mesenchymal Stromal Cell Proliferation via Targeting the Coding Sequence of Abca4

et al. 2021

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Amniotic fluid-derived mesenchymal stromal cells (AFMSCs) present different features, depending on the isolation timing and culture conditions. The lack of uniform experimental standards hinders the comparison of results from different studies on AFMSCs. Moreover, understanding the molecular mechanisms that underlie the features of AFMSCs isolated at different embryonic developmental stages might allow the obtention of more viable and highly proliferative AFMSCs through genetic modification. We isolated AFMSCs from pregnant rats at embryonic day (E)12, E15, E18, and E21 and compared their cell proliferation capacity and transcriptome. The cell counting kit-8 assay and RNA sequencing revealed that E12 and E15 AFMSCs showed different characteristics from E18 and E21 AFMSCs. Therefore, AFMSCs were divided into two groups: early (E12 and E15) and late (E18 and E21) pregnancy-stage groups. Next, we screened the gene/microRNA pair Abca4/miR-351-3p that was related to cell proliferation. Abca4 knockdown/overexpression suggested that this gene represses the proliferation of AFMSCs, which is a newly discovered function of this gene. Finally, dual luciferase reporter gene assays confirmed that miR-351-3p targeted the coding sequence of Abca4 and regulated AFMSC proliferation. miR-351-3p promotes AFMSC proliferation via targeting the coding sequence of Abca4. Our findings provide a molecular foundation for further research for obtaining AFMSCs with a higher proliferation capacity.

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Rat full term amniotic fluid harbors highly potent stem cells

Cited by 14 publications

References 63 publications

Prospective stem cell lines as in vitro neurodegenerative disease models for natural product research

Prospective stem cell lines as in vitro neurodegenerative disease models for natural product research

Accelerated wound closure: Systematic evaluation of cellulose acetate effects on biologically active molecules release from amniotic fluid stem cells

miR-351-3p Promotes Rat Amniotic Fluid-Derived Mesenchymal Stromal Cell Proliferation via Targeting the Coding Sequence of Abca4

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