Rat Erythrocyte Susceptibility to Lipid Peroxidation After Chronic Ethanol Intake

Lindi, C.; Montorfano, G.; Marciani, P.

doi:10.1016/s0741-8329(98)00020-2

Cited by 26 publications

(11 citation statements)

References 37 publications

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“…Moreover, changes in fatty acid composition that takes place during ethanol intake may make membrane lipids more liable to peroxidation. Both membrane physicochemical changes and free-radical induced lipid peroxidation are probably involved in several physiological and/or pathological events such as the altering of structural and functional membrane features [17]. To the best of our knowledge, the influence of EA on histological changes induced by ethanol has not yet been studied.…”

Section: Introductionmentioning

confidence: 99%

Modulatory potential of ellagic acid, a natural plant polyphenol on altered lipid profile and lipid peroxidation status during alcohol‐induced toxicity: A pathohistological study

Nagarajan

Sudheer

Vishwanathan

et al. 2008

J Biochem & Molecular Tox

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Polyphenol-rich dietary foodstuffs, consumed as an integral part of vegetables, fruits, and beverages have attracted attention due to their antioxidant and anticancer properties. Ellagic acid (EA), a polyphenolic compound widely distributed in fruits and nuts, has been reported to scavenge free radicals and inhibit lipid peroxidation. Chronic consumption of alcohol potentially results in serious illness including hepatitis, fatty liver, hypertriglyceridemia, and cirrhosis. A little is known about the influence of EA on alcohol toxicity in vivo. Accordingly, in the present study, we have evaluated the protective effects of EA on lipid peroxidation and lipid levels during alcohol-induced toxicity in experimental rats. Forty female albino Wistar rats, which were weighing between 150-170 g were used for the study. The toxicity was induced by administration of 20% alcohol orally (7.9 g/kg body wt.) for 45 days. Rats were treated with EA at three different doses (30, 60, and 90 mg/kg body wt.) via intragastric intubations together with alcohol. At the end of experimental duration, liver marker enzymes (i.e., aspartate transaminase, alanine transaminase), lipid peroxidative indices (i.e., thiobarbituriacid reactive substances and hydroperoxides) in plasma, and lipid levels (i.e., cholesterol, free fatty acids, triglycerides and phospholipids) in tissues were analyzed to evaluate the antiperoxidative and antilipidemic effects of EA. Liver marker enzymes, lipid peroxidative indices, and lipid levels, i.e., cholesterol, triglycerides and free fatty acids, were significantly increased whereas phospholipid levels were significantly decreased in the alcohol-administered group. EA treatment resulted in positive modulation of marker enzymes, peroxidative indices, and lipid levels. EA at the dose of 60 mg/kg body wt. was found to be more effective when compared to the other two doses. Histological changes observed were also inconsistent with the biochemical parameters. Our study suggests that EA exerts beneficial effects at the dosage of 60 mg/kg body wt. against alcohol-induced damage, and it can be used as a potential drug for the treatment of alcohol-abuse ailments in the near future.

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Section: Introductionmentioning

confidence: 99%

Modulatory potential of ellagic acid, a natural plant polyphenol on altered lipid profile and lipid peroxidation status during alcohol‐induced toxicity: A pathohistological study

Nagarajan

Sudheer

Vishwanathan

et al. 2008

J Biochem & Molecular Tox

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“…Experimental evidence has shown that ethanol exerts some of its pharmacological effects by modifying the lipid composition of the membranes and consequently altering their fluidity [10,11]. Thus, it has been postulated that as far as some membranes are concerned an acute dose of ethanol disorders the lipid bilayer while chronic exposure to ethanol produces an increase in the order of the membrane.…”

Section: Introductionmentioning

confidence: 99%

Chronic ingestion of ethanol stimulates lipogenic response in rat hepatocytes

2001

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We isolated hepatocytes from rats chronically fed with ethanol and pair-fed control rats and incubated them both in the presence and absence of 100 mM ethanol in order to analyze the uptake into their lipids of several radiolabeled exogenous substrates. The hepatocytes treated chronically with ethanol showed higher lipogenic activity both in neutral lipids and phospholipids from serine, ethanolamine, glycerol and oleate. The only exception found was in the incorporation of choline into phosphatidylcholine (PC), which was lower in the hepatocytes from ethanol-fed rats than in the controls and was concomitant with a decrease in the PC levels of the ethanol-fed hepatocytes. The results obtained after exposing the cells to 100 mM ethanol in vitro indicate that in general the hepatocytes from ethanol-fed rats exhibit a higher lipogenic activity than the control cells. The only difference in the response to ethanol in vitro was found in the biosynthesis of phosphatidylserine (PS) from serine, which rose significantly in control cells but was unaffected in alcoholic hepatocytes. We put this difference in response down to specific adaptation to ethanol feeding.

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“…Our data are in accordance with some previous studies suggesting that erythrocyte membranes of alcoholic animals are more sensitive to LPO since they are rich in polyunsaturated fatty acids, molecular oxygen and ferrous ions all of which provide favourable conditions for lipoperoxidative damage. 40 However, we suggest that further studies focused on the haemolytic effects of protein-acetaldehyde adducts produced in the erythrocytes of alcoholics should clarify this point.…”

Section: Resultsmentioning

confidence: 91%

“…39 Our results showing significantly elevated lipid peroxidation in erythrocyte lysates and membranes are in good agreement with the previous studies. 8,9,[16][17][18]40,41 It has been proposed that lipid peroxidation is initiated by a free radical extracting a hydrogen atom from a reactive methylene group of a fatty acid. The resulting low molecular weight secondary products, e.g.…”

Section: Resultsmentioning

confidence: 99%

Lipid peroxidation and antioxidant enzyme activities in erythrocytes of type I and II alcoholics

Uçar¹,

Demir²,

Uluğ³

2004

Cell Biochem. Funct.

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Reduced and oxidized glutathione (GSH and GSSG), protein-bound glutathione, lipid peroxidation and antioxidant enzyme activities were determined in the erythrocyte lysates and membranes of type I and II alcoholics in order to clarify the effect of age-of-onset and the duration of the alcohol consumption on erythrocyte oxidant and antioxidant status. The osmotic fragility and susceptibility of the erythrocytes to haemolysis were also determined. Erythrocyte lipid peroxidation was significantly increased but, GSH and protein-bound GSH, GSH/GSSG ratio and antioxidant enzyme activities were markedly decreased in the erythrocytes of the alcoholic subgroups. Erythrocyte count and haemoglobin content in the blood of alcoholics were found to be decreased in accordance with the finding that erythrocytes were more fragile and less resistant to haemolysis particularly in type II alcoholics. The present study showed that ethanol-induced oxidative stress in erythrocytes can lead to haemolysis and membrane-specific injuries in erythrocytes of the alcoholic subtypes.

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Rat Erythrocyte Susceptibility to Lipid Peroxidation After Chronic Ethanol Intake

Cited by 26 publications

References 37 publications

Modulatory potential of ellagic acid, a natural plant polyphenol on altered lipid profile and lipid peroxidation status during alcohol‐induced toxicity: A pathohistological study

Modulatory potential of ellagic acid, a natural plant polyphenol on altered lipid profile and lipid peroxidation status during alcohol‐induced toxicity: A pathohistological study

Chronic ingestion of ethanol stimulates lipogenic response in rat hepatocytes

Lipid peroxidation and antioxidant enzyme activities in erythrocytes of type I and II alcoholics

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