Rapidly progressive glomerulonephritis due to coexistent anti-glomerular basement membrane and anti-myeloperoxidase antibody

Cheng, Chung Yi; Chen, Tso Hsiao; Sue, Yuh Moe; Lee, Wen Sen

doi:10.1016/j.jmii.2016.11.009

Cited by 2 publications

(1 citation statement)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thirty Wistar-Kyoto (WKY) rats were randomly divided into three groups ( n = 10/group) the control group; the MPO-AAV group in which rats were given a one-time intraperitoneal injection of 400 µg/kg MPO (MPO dissolved in sterile water for injection at a concentration of 500 µg/ml, then mixed with an equal amount of Freund’s complete adjuvant [ 23 – 27 ]; and the MPO-AAV + anti-FKN group, in which rats were given a single intraperitoneal injection of 400 µg/kg MPO for 6 weeks, then anti-FKN (1 µg/ rat /day, i.p) [ 28 , 29 ]. One week later, orbital blood was collected on EDTA-K2 anticoagulant tube, and the rats were killed by disjoint method.…”

Section: Methodsmentioning

confidence: 99%

Expression and significance of Fractalkine/CX3CL1 in MPO-AAV-associated glomerulonephritis rats

Ma,

Wang,

Kang

et al. 2024

BMC Nephrol

View full text Add to dashboard Cite

Objective To investigate the expression and significance of Fractalkine (CX3CL1, FKN) in serum and renal tissue of myeloperoxidase and anti-neutrophil cytoplasmic antibody associated vasculitis (MPO-AAV) rats. Methods Thirty Wistar-Kyoto (WKY) rats were randomly divided into: Control group, MPO-AAV group (400 µg/kg MPO mixed with Freund’s complete adjuvant i.p), MPO-AAV + Anti-FKN group (400 µg/kg MPO mixed with Freund’s complete adjuvant i.p), anti-FKN group (1 µg/ rat /day, i.p) after 6 weeks. MPO-AAV associated glomerulonephritis model was established by intraperitoneal injection of MPO + Freund’s complete adjuvant with 10 mice in each group. The concentration of MPO-ANCA and FKN in serum was detected by Enzyme-linked immunosorbent assay (ELISA). Hematoxylin-eosin (HE) staining was used to detect pathological changes of kidney tissue. Western blot and immunofluorescence staining were used to detect the expression and localization of FKN protein in kidney tissue. Renal function test indicators: 24-hour urinary protein (UAER), blood urea nitrogen (BUN), serum creatinine (Scr). The expression levels of p65NF-κB and IL-6 was detected by Immunohistochemical assays. Results Compared with the control group, the serum MPO-ANCA antibody expression level in the MPO-AAV group was significantly increased (P < 0.01), and the contents of UAER, BUN and Scr were significantly up-regulated at 24 h (P < 0.01). Compared with the control group, the glomeruli in the MPO-AAV group had different degrees of damage, infiltration of inflammatory cell, and membrane cell hyperplasia and renal tubule edema. Compared with the control group, rats in the MPO-AAV group had significantly higher levels of FKN in serum and renal tissues (P < 0.01), and high expression of p65NF-κB and IL-6 in renal tissues (P < 0.01) (P < 0.05), whereas anti-FKN reversed the expression of the above factors. In MPO-AAV renal tissue, FKN was mainly expressed in the cytoplasm of renal tubular epithelial cells and glomerular podocytes. In addition, the contents of 24 h UAER, BUN and Scr of renal function in MPO-AAV rats were significantly decreased (P < 0.01) and the damage of renal tissue was significantly ameliorated after the administration of antagonistic FKN. Conclusion FKN may play a key role in the pathogenesis of MPO-AAV associated glomerulonephritis.

show abstract

Section: Methodsmentioning

confidence: 99%