Rapidec Carba NP for Detection of Carbapenemase-Producing Enterobacteriaceae in Clinical Isolates: A Cross-Sectional Study

Elawady, Basma; Ghobashy, Marwa; Balbaa, Amal

doi:10.1089/sur.2019.084

Cited by 10 publications

(7 citation statements)

References 26 publications

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“…Previous studies found the efflux pump by the molecular technique such as PCR and observed that AcrAB efflux pump and mdtK efflux pump existed in CRE isolates. 23,41 In the current study, carbapenemase enzymes were detected by Carba NP test and MHT in 98% and 95%, respectively, which was in line with another study carried by Elawady et al 44 and Garg et al 45 who reported good sensitivity and specificity of Carba NP test.…”

Section: Discussionsupporting

confidence: 91%

Evaluation of Resistance Mechanisms in Carbapenem-Resistant Enterobacteriaceae

Alizadeh¹,

Rezaee²,

Kafil³

et al. 2020

IDR

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Background: Carbapenem-resistant Enterobacteriaceae (CRE) is a major concern leading to morbidity and mortality in the world. CRE often is becoming a cause of therapeutic failure in both hospital and community-acquired infections. Aim: This study aimed to investigate the resistance mechanisms of CRE by phenotypic and molecular methods. Materials and Methods: Sixty CRE (50 Klebsiella pneumoniae, 6 Escherichia coli, and 4 Enterobacter spp.) were isolated from October 2018 to June 2019. Antimicrobial susceptibility testing was carried out using phenotypic methods. The carbapenem resistance mechanisms including efflux pump hyperexpression, AmpC overproduction, carbapenemase genes, and deficiency in OmpK35 and OmpK36 were determined by phenotypic and molecular methods, respectively. Results: Sixty CRE (50 Klebsiella pneumoniae, 6 Escherichia coli, and 4 Enterobacter spp.) were isolated from October 2018 to June 2019. Amikacin was found to be the most effective drug against CRE isolates. All isolates were resistant to imipenem and meropenem by the micro-broth dilution. AmpC overproduction was observed in all Enterobacter spp. and three K. pneumoniae isolates. No efflux pump activity was found. Carba NP test and Modified Hodge Test could find carbapenemase in 59 (98%) isolates and 57 (95%) isolates, respectively. The most common carbapenemase gene was bla OXA-48-like (72.8%) followed by bla NDM (50.8%), bla IMP (18.6%), bla VIM (11.8%), and bla KPC (6.7%). The ompK35 and ompK36 genes were not detected in 10 and 7 K. pneumoniae isolates, respectively. Conclusion: The amikacin is considered as a very efficient antibiotic for the treatment of CRE isolates in our region. Carbapenemase production and overproduction of AmpC are the main carbapenem resistance mechanisms in CRE isolates. Finally, Carba NP test is a rapid and reliable test for early detection of carbapenemase-producing isolates.

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Section: Discussionsupporting

confidence: 91%

Evaluation of Resistance Mechanisms in Carbapenem-Resistant Enterobacteriaceae

Alizadeh¹,

Rezaee²,

Kafil³

et al. 2020

IDR

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“…Additional equipment and software are required for both MALDI-TOF hydrolysis testing and molecular analysis. Detection of carbapenemases from blood cultures using an immunochromatographic assay has been recently reported [30][31][32][33] .…”

Section: Discussionmentioning

confidence: 99%

Rapid detection of carbapenemases and ESKAPEEc bacteria

Aydoğan¹,

Çakırlar²

2021

Arch Balk Med Union

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Détection rapide des carbapénémases et des bactéries ESKAPEEcIntroduction. Les carbapénèmes sont les antibiotiques de dernière génération pour le traitement des infections causées par des bactéries multirésistantes. Cependant, récemment, des bactéries résistantes aux carbapénèmes ont émergé de manière significative. L'objectif de l'étude. Nous visons une étude préliminaire pour l'identification des souches pathogènes

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“…Both version's results are interpreted in less than 2 hours by the naked eye (color change). Many studies evaluated the accuracy of the commercial version, the sensitivity and the specificity were varying between 90-100% and 84-100% respectively 37,[48][49][50] .…”

Section: Discussionmentioning

confidence: 99%

Diagnostic Accuracy of Carba NP test for Rapid Detection of Carbapenemase-Producing Enterobacteriaceae: Systematic Review and Meta-Analysis

Zefenkey,

Saleem,

Mohammed

2021

MLU

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Background: Carbapenem-Resistant Enterobacteriaceae is a major health concern that needs a fast response by rapid detection and management. The Polymerase chain reaction is the gold standard method for the detection of this infection, but it is expensive and needs special laboratory facilities, so there is an urgent demand for an alternative method characterized by cheap, rapid, and accurate. CARPA NP test has been proposed as a rapid method for the detection of Carbapenemase-Producing Enterobacteriaceae, but its overall accuracy has not been systematically estimated. We performed a systematic review and meta-analysis to evaluate the accuracy of CARPA NP test for the detection of Carbapenemase-Producing Enterobacteriaceae.Methods: We searched Pubmed and Embase databases and eighteen studies met our inclusion criteria. We utilized summary receiver operating characteristic (SROC) to summarize the accuracy. Results: The sensitivity and specificity of CARPA NP test were 0.96 (95% CI, 0.92 to 0.98) and 1.00 (95% CI, 0.97 to 1.00) respectively, the positive likelihood ratio was 33.1 (95% CI, 24.3 to 45.1), the negative likelihood ratio was 0.06 (95% CI, 0.05 to 0.07), and the diagnostic odds ratio was 573.46 (95% CI, 397.4 to 827.49). Conclusion:CARPA NP test has a high level of accuracy for the rapid detection of Carbapenemase-Producing Enterobacteriaceae. Additional studies are required to estimate the accuracy of Carba NP test when applied directly to clinical samples, especially in areas with high rates of carbapenemase-producing Enterobacteriaceae

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Rapidec Carba NP for Detection of Carbapenemase-Producing Enterobacteriaceae in Clinical Isolates: A Cross-Sectional Study

Cited by 10 publications

References 26 publications

<p>Evaluation of Resistance Mechanisms in Carbapenem-Resistant <em>Enterobacteriaceae</em></p>

<p>Evaluation of Resistance Mechanisms in Carbapenem-Resistant <em>Enterobacteriaceae</em></p>

Rapid detection of carbapenemases and ESKAPEEc bacteria

Diagnostic Accuracy of Carba NP test for Rapid Detection of Carbapenemase-Producing Enterobacteriaceae: Systematic Review and Meta-Analysis

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