Rapid titrimetric and spectrophotometric assay methods for the determination of lamivudine in pharmaceuticals using iodate and two dyes

Basavaiah, K.; Somashekar, B. C.; Ramakrishna, V.

doi:10.1134/s1061934807060081

Cited by 12 publications

(16 citation statements)

References 10 publications

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“…Lamivudine and zidovudine are present in the popular HIV medication Combivir™. Spectrophotometric (6)(7)(8)(9), chromatographic [10][11][12][13][14][15][16] and electrochemistry (17)(18) analytical methods have been developed for the determination of lamivudine. As a result of advancements in properties of the adsorptive stripping voltammetry, new methodologies have been developed for adenine, thymine, guanine, ATP, DNA and antiretroviral drugs determinations, employing alkaline solution with lower ionic strength as the supporting electrolyte (19)(20)(21)(22)(23)(24)(25).…”

Section: Introductionmentioning

confidence: 99%

Ultratrace Determination of the Antiretroviral Drug Lamivudine in Diluted Alkaline Electrolyte by Adsorptive Stripping Voltammetry

2012

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Section: Introductionmentioning

confidence: 99%

Ultratrace Determination of the Antiretroviral Drug Lamivudine in Diluted Alkaline Electrolyte by Adsorptive Stripping Voltammetry

2012

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“…Various spectrophotometric [1][2][3][4][5][6] methods, titrimetric and spectrophotometric methods [7,8] ,…”

Section: World Journal Of Pharmaceutical Researchmentioning

confidence: 99%

A Simple Spectrophotometric Assay of Lamivudine in Pharmaceutical Formulations

Reddy¹

2017

WJPR

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A simple, sensitive and accurate spectrophotometric method has been developed for the estimation of lamivudine in bulk and pharmaceutical dosage forms. The amino group in lamivudine is diazotised with sodium nitrite and hydrochloric acid at 0 o C temperature. After diazotisation, the diazonium salt is coupled with resorcinol. The orange red coloured chromogen formed in the method is stable for more than 24 hours. The orange red coloured chromogen is measured at the wavelength of maximum absorbance 460 nm against the reagent blank.The results obtained with the proposed method are in good agreement with labeled amounts, when marketed pharmaceutical preparations are analyzed.

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“…Several high-performance liquid chromatographic (HPLC) [5][6][7][8][9][10] and spectrophotometric [11,12] methods for the determination of lamivudine in biological fluids have been published. Recently, electroanalytical methods [13,14] for lamivudine determination in human serum and plasma have been developed.…”

Section: Introductionmentioning

confidence: 99%

Differential Pulse Voltammetric Studies on Lamivudine: An Antiretroviral Drug

Leandro¹,

Moreira²,

Farias³

2013

AJAC

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Lamivudine (also known as 3TC) is a dideoxynucleoside analogue, which undergoes intracellular phosphorylation in the putative active metabolite, lamivudine triphosphate. Lamivudine triphosphate prevents HIV replication by competitively inhibiting viral reverse transcriptase. Lamivudine has been extensively used in the treatment of HIV patients owing to its antiretroviral activity. For the determination of lamivudine in pharmaceuticals, an analytical methodology using voltammetry was developed. Lamivudine was reduced at a hanging mercury drop electrode (HMDE) at -1.16 V vs Ag/AgCl at pH 2.0. The influence of electroanalytical parameters such as scan rate (20 mV·s -1 ), amplitude (50 mV), nature of the support electrolyte (Clark-Lubs), and pH (2.0) on the voltammetric signal was optimized. Under these optimized conditions, the method had been validated using pharmaceutical formulations. The lamivudine peak current varied linearly with its concentration from 1.15 to 10.40 mg·L -1 , detection and determination limits of 0.46 and 1.0 mg·L -1 , respectively, and recovery of 95.15% with a relative standard deviation of 1.10%.

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Rapid titrimetric and spectrophotometric assay methods for the determination of lamivudine in pharmaceuticals using iodate and two dyes

Cited by 12 publications

References 10 publications

Ultratrace Determination of the Antiretroviral Drug Lamivudine in Diluted Alkaline Electrolyte by Adsorptive Stripping Voltammetry

Ultratrace Determination of the Antiretroviral Drug Lamivudine in Diluted Alkaline Electrolyte by Adsorptive Stripping Voltammetry

A Simple Spectrophotometric Assay of Lamivudine in Pharmaceutical Formulations

Differential Pulse Voltammetric Studies on Lamivudine: An Antiretroviral Drug

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