Rapid three-dimensional biointerfaces for real-time immunoassay using hIL-18BPa as a model antigen

Carrigan, Shawn D.; Scott, George M.; Tabrizian, Maryam

doi:10.1016/j.biomaterials.2005.05.089

Cited by 21 publications

(22 citation statements)

References 29 publications

(31 reference statements)

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“…17 Furthermore, PEG-diamines were used as branch molecules in the network which were linked to cell-active molecules. 18 Other interesting hydrogels in tissue engineering approaches are polyethyleneimine composites 19,20 and carboxymethyl dextran 21 . They are characterized by low cytotoxicity, simple processing, and variable applicability.…”

Section: Introductionmentioning

confidence: 99%

Plasma functionalization of polycarbonaturethane to improve endothelialization—Effect of shear stress as a critical factor for biocompatibility control

Lukas

Thomas²,

Gessner

et al. 2016

J Biomater Appl

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Medical devices made of polycarbonaturethane (PCU) combine excellent mechanical properties and little biological degradation, but restricted hemocompatibility. Modifications of PCU might reduce platelet adhesion and promote stable endothelialization. PCU was modified using gas plasma treatment, binding of hydrogels, and coupling of cellactive molecules (modified heparin, anti-thrombin III (ATIII), argatroban, fibronectin, laminin-nonapeptide, peptides with integrin-binding arginine-glycine-aspartic acid (RGD) motif). Biocompatibility was verified with static and dynamic cell culture techniques. Blinded analysis focused on improvement in endothelial cell (EC) adhesion/proliferation, antithrombogenicity, reproducible manufacturing process, and shear stress tolerance of ECs. EC adhesion and antithrombogenicity were achieved with 9/35 modifications. Additionally, 6/9 stimulated EC proliferation and 3/6 modification processes were highly reproducible for endothelialization. The latter modifications comprised immobilization of ATIII (A), polyethyleneglycole-diamine-hydrogel (E) and polyethylenimine-hydrogel connected with modified heparin (IH). Under sheer stress, only the IH modification improved EC adhesion within the graft. However, ECs did not arrange in flow direction and cell anchorage was restricted. Despite large variation in surface modification chemistry and improved EC adhesion under static culture conditions, additional introduction of shear stress foiled promising preliminary data. Therefore, biocompatibility testing required not only static tests but also usage of physiological conditions such as shear stress in the case of vascular grafts.

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Section: Introductionmentioning

confidence: 99%

Plasma functionalization of polycarbonaturethane to improve endothelialization—Effect of shear stress as a critical factor for biocompatibility control

Lukas

Thomas²,

Gessner

et al. 2016

J Biomater Appl

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“…Other clinical marker assays reported include those for bone morphogenic protein-2 (Michalzik et al, 2005), plasma vitellogenin (Oshima et al, 2005), cholinesterase (Halamek et al, 2005), a-fetoprotein (Chou et al, 2002a;Tsai and Lin, 2005), human ferritin (Chou et al, 2002b), transferrin (Wang et al, 2001a), various general sepsis markers (Carrigan et al, 2005a(Carrigan et al, , 2005b, thrombin (Wang et al, 2002b) and ceruloplasmin (Wang et al, 2004b). Smaller polypeptide analytes detected by piezoelectric immunoassays include insulin (Saha et al, 2002) and glutathione-protected nanoclusters (Gerdon et al, 2005).…”

Section: Clinical Analysismentioning

confidence: 99%

A survey of the 2001 to 2005 quartz crystal microbalance biosensor literature: applications of acoustic physics to the analysis of biomolecular interactions

Cooper¹,

Singleton²

2007

J of Molecular Recognition

335

205

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The widespread exploitation of biosensors in the analysis of molecular recognition has its origins in the mid-1990s following the release of commercial systems based on surface plasmon resonance (SPR). More recently, platforms based on piezoelectric acoustic sensors (principally 'bulk acoustic wave' (BAW), 'thickness shear mode' (TSM) sensors or 'quartz crystal microbalances' (QCM)), have been released that are driving the publication of a large number of papers analysing binding specificities, affinities, kinetics and conformational changes associated with a molecular recognition event. This article highlights salient theoretical and practical aspects of the technologies that underpin acoustic analysis, then reviews exemplary papers in key application areas involving small molecular weight ligands, carbohydrates, proteins, nucleic acids, viruses, bacteria, cells and lipidic and polymeric interfaces. Key differentiators between optical and acoustic sensing modalities are also reviewed.

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“…Antibody-antigen-based detection of target molecules has gained attention in the last three decades due to its high specificity and low detection limit. 128 This hydrogel-interface has been evaluated with detection of cytokines rhIL-1ra, rhIL-6, and rhiL-18BPa antigens. 127 Detection in antibody-antigen sensing system can be performed in different ways.…”

Section: Antibody-antigenmentioning

confidence: 99%

Hydrogels in Biosensing Applications

Topuz

Buenger

Tanaka

et al. 2011

Comprehensive Biomaterials

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Rapid three-dimensional biointerfaces for real-time immunoassay using hIL-18BPa as a model antigen

Cited by 21 publications

References 29 publications

Plasma functionalization of polycarbonaturethane to improve endothelialization—Effect of shear stress as a critical factor for biocompatibility control

Plasma functionalization of polycarbonaturethane to improve endothelialization—Effect of shear stress as a critical factor for biocompatibility control

A survey of the 2001 to 2005 quartz crystal microbalance biosensor literature: applications of acoustic physics to the analysis of biomolecular interactions

Hydrogels in Biosensing Applications

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