Rapid Surveillance for Vector Presence (RSVP): Development of a novel system for detecting Aedes aegypti and Aedes albopictus

Montgomery, Brian L.; Shivas, Martin A.; Hall‐Mendelin, Sonja; Edwards, Jim; Hamilton, Nicholas A.; Jansen, Cassie C.; McMahon, Jamie; Warrilow, David; Hurk, Andrew F. van den

doi:10.1371/journal.pntd.0005505

Cited by 24 publications

(26 citation statements)

References 81 publications

(76 reference statements)

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“…New surveillance and control measures introduced in recent years may influence how a similar response would be approached under current circumstances. The use of molecular testing and subsequent expenses for adult/larval/egg collections (Montgomery et al 2017) may be a quick, easier, and cheaper surveillance tool, especially for larval presence, by taking water samples in rainwater tanks, other receptacles, and storm water drain systems. If the incursion had not been eliminated, Ae.…”

Section: Discussionmentioning

confidence: 99%

Elimination of Aedes aegypti in northern Australia, 2004–2006

Whelan¹,

Kurucz²,

Pettit³

et al. 2020

Journal of Vector Ecology

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The Northern Territory (NT) of Australia is currently free of the dengue mosquito Aedes (Stegomyia) aegypti (L). However, on 17 February 2004, two Ae. aegypti adults were captured in two routine CO2‐baited encephalitis virus surveillance traps in Tennant Creek, located 990 km south of Darwin in the NT. The detection triggered an immediate survey and control response undertaken by the NT Department of Health and Community Services, followed by a Commonwealth of Australia‐funded Ae. aegypti elimination program. This report details the methods and results of the detection and subsequent elimination activities that were carried out between 2004 and 2006, returning the NT to its dengue vector‐free status. There have been very few successful Ae. aegypti elimination programs in the world. This purposeful mosquito elimination for Australia was officially declared on 5 April 2006.

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Section: Discussionmentioning

confidence: 99%

Elimination of Aedes aegypti in northern Australia, 2004–2006

Whelan¹,

Kurucz²,

Pettit³

et al. 2020

Journal of Vector Ecology

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“…A sample was deemed positive when its corresponding LAMP reaction was yellow in colour and the accompanying NTC reaction was negative (purple colour). A fluorescent probe PCR-based assay (TaqMan) was used for sensitivity comparison [13].…”

Section: Colorimetric Lamp Assaymentioning

confidence: 99%

“…aegypti and Ae. albopictus, and exist in classical [11] and realtime platforms [12][13][14]. These assays can be used to differentiate between species which share overlapping morphology, such as Ae.…”

Section: Introductionmentioning

confidence: 99%

“…Molecular analyses can also be used to identify field samples damaged during the collection process or pupal exuviae, a scenario faced by quarantine personnel inspecting cargo receptacles at ports of first entry. Finally, molecular identification of 1 st instar larvae is a critical part of a novel system to expedite presence/absence surveys for both of these invasive mosquitoes, the Rapid Surveillance for Vector Presence (RSVP) [13]. In this system, thousands of eggs from multiple ovitrap collections are batched in cohorts of up to 5,000 eggs and analysed using TaqMan PCR.…”

Section: Introductionmentioning

confidence: 99%

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A LAMP-based colorimetric assay to expedite field surveillance of the invasive mosquito species Aedes aegypti and Aedes albopictus

et al. 2020

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Background Yellow fever, dengue, chikungunya and Zika viruses are responsible for considerable morbidity and mortality in humans. Aedes aegypti and Aedes albopictus are the most important mosquito vectors involved in their transmission. Accurate identification of these species is essential for the implementation of control programs to limit arbovirus transmission, during suspected detections at ports of first entry, to delimit incursions or during presence/absence surveillance programs in regions vulnerable to invasion. We developed and evaluated simple and rapid colorimetric isothermal tests to detect these two mosquito species based on loop-mediated isothermal amplification (LAMP) targeting the ribosomal RNA internal transcribed spacer 1 (ITS1). Methodology/Principal findings Samples were prepared by homogenizing and heating at 99 o C for 10 min before an aliquot was added to the LAMP reaction. After 40 min incubation at 65 o C, a colour change indicated a positive result. The tests were 100% sensitive and species-specific, and demonstrated a limit of detection comparable with PCR-based detection (TaqMan chemistry). The LAMP assays were able to detect target species for various life stages tested (adult, 1 st instar larva, 4 th instar larva and pupa), and body components, such as legs, wings and pupal exuviae. Importantly, the LAMP assays could detect Ae. aegypti DNA in mosquitoes stored in Biogents Sentinel traps deployed in the field for 14 d. A single 1 st instar Ae. aegypti larva could also be detected in a pool of 1,000 non-target 1 st instar Aedes notoscriptus, thus expediting processing of ovitrap collections obtained during presence/absence surveys. A simple syringe-sponge protocol facilitated the concentration and collection of larvae from the ovitrap water post-hatch.

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“…Citizen science is also an alternative [44]. The Zika Mozzie Seeker programme in Queensland relies on citizens to obtain Aedes eggs using ovitraps [45]. The ovistrips are then posted to a laboratory for PCR analysis.…”

Section: Hurdles To Successful Implementation At Scalementioning

confidence: 99%

Reflections from an old Queenslander: can rear and release strategies be the next great era of vector control?

Ritchie

Staunton

2019

Proc. R. Soc. B.

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In this perspective, I discuss the great eras of vector control, centring on Aedes aegypti , the primary vector of dengue, Zika and several other viruses. Since the discovery and acceptance of the role of mosquitoes as vectors of disease agents, several significant strategies have been developed and deployed to control them and the diseases they transmit. Environmental management, insecticides and, to a lesser extent, biological control have emerged as great eras of vector control. In the past decade, the release of massive numbers of specifically modified mosquitoes that mate with wild populations has emerged as a significant new strategy to fight vector-borne diseases. These reared and released mosquitoes have been modified by the addition of a symbiont (e.g. Wolbachia bacteria), radiation or introduction of a genetic construct to either sterilize the wild mosquitoes they mate with, crashing the population, or to reduce the wild population's capacity to vector pathogens. Will these new rear and release strategies become the next great era of vector control? From my vantage point as a dengue control manager and researcher involved in two Wolbachia programmes, I will discuss the hurdles that rear and release programmes face to gain widespread acceptance and success.

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Rapid Surveillance for Vector Presence (RSVP): Development of a novel system for detecting Aedes aegypti and Aedes albopictus

Cited by 24 publications

References 81 publications

Elimination of Aedes aegypti in northern Australia, 2004–2006

Elimination of Aedes aegypti in northern Australia, 2004–2006

A LAMP-based colorimetric assay to expedite field surveillance of the invasive mosquito species Aedes aegypti and Aedes albopictus

Reflections from an old Queenslander: can rear and release strategies be the next great era of vector control?

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