Rapid, specific and sensitive electrochemical detection of foodborne bacteria

Pöhlmann, Christopher; Wang, Yiran; Humeník, Martin; Heidenreich, Bernd; Gareis, M.; Sprinzl, Mathias

doi:10.1016/j.bios.2009.01.042

Cited by 60 publications

(43 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Leading the way on portability have been research groups such as Ligler et al [40], Poehlmann et al [61], Banerjee and Bhunia [48] and Mata et al [67] with pathogen detection, Yang et al [75], Mak et al [76] and Stevens et al [38] with toxin detection and Hildebrandt et al [118] with pesticide detection. Further research into sample preparation techniques must be performed to help make more systems that are truly portable from the laboratory.…”

Section: Resultsmentioning

confidence: 99%

“…Several articles have been published showing biosensor methods that incorporate a PCR step [60,61]. Chen et al [60] immobilised an oligonucleotide specific for a gene of E. coli O157:H7 onto a mass/acoustic (piezoelectric) transducer in their flow-based biosensor.…”

Section: Biosensors Utilising Polymerase Chain Reactionmentioning

confidence: 99%

“…e.g. electrochemical (amperometric) [61], optical (luminescence [33] and SPR [62]), mass/acoustic (piezoelectric) [60] and mass (cantilever) [63] transducers, although some are very much proof-of-concept biosensor that require more work to prove their use with actual food samples. Some biosensor articles have described sample preparations that have attempted to remove the time- consuming enrichment or culturing steps by using magnetic particles.…”

Section: Biosensors For Pathogen Detectionmentioning

confidence: 99%

“…This gold label amplified the signal, providing a LOD of 5.3×10 2 CFU/ml without enrichment. Sample preparation and analysis took 3 h. Poehlmann et al [61] used an electrochemical (amperometric) portable flowbased biosensor for the detection of E. coli in meat juice. They immobilised a specific nucleotide sequence to capture the amplified 16S ribosomal RNA that had been isolated from the bacteria using an RNeasy Mini kit.…”

Section: Biosensors Utilising Polymerase Chain Reactionmentioning

confidence: 99%

See 3 more Smart Citations

Biosensors for the analysis of microbiological and chemical contaminants in food

2012

View full text Add to dashboard Cite

Increases in food production and the ever-present threat of food contamination from microbiological and chemical sources have led the food industry and regulators to pursue rapid, inexpensive methods of analysis to safeguard the health and safety of the consumer. Although sophisticated techniques such as chromatography and spectrometry provide more accurate and conclusive results, screening tests allow a much higher throughput of samples at a lower cost and with less operator training, so larger numbers of samples can be analysed. Biosensors combine a biological recognition element (enzyme, antibody, receptor) with a transducer to produce a measurable signal proportional to the extent of interaction between the recognition element and the analyte. The different uses of the biosensing instrumentation available today are extremely varied, with food analysis as an emerging and growing application. The advantages offered by biosensors over other screening methods such as radioimmunoassay, enzyme-linked immunosorbent assay, fluorescence immunoassay and luminescence immunoassay, with respect to food analysis, include automation, improved reproducibility, speed of analysis and real-time analysis. This article will provide a brief footing in history before reviewing the latest developments in biosensor applications for analysis of food contaminants (January 2007 to December 2010), focusing on the detection of pathogens, toxins, pesticides and veterinary drug residues by biosensors, with emphasis on articles showing data in food matrices. The main areas of development common to these groups of contaminants include multiplexing, the ability to simultaneously analyse a sample for more than one contaminant and portability. Biosensors currently have an important role in food safety; further advances in the technology, reagents and sample handling will surely reinforce this position.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Biosensors Utilising Polymerase Chain Reactionmentioning

confidence: 99%

Section: Biosensors For Pathogen Detectionmentioning

confidence: 99%

Section: Biosensors Utilising Polymerase Chain Reactionmentioning

confidence: 99%

See 2 more Smart Citations

Biosensors for the analysis of microbiological and chemical contaminants in food

2012

View full text Add to dashboard Cite

show abstract

“…As an example, multiplex DNA detection was obtained employing multiple Au particle-on-wire systems as a SERS sensing platform [58]. Selected recent applications of biosensors to multiplexed bacteria analysis in food samples are reported in Table 1 [58][59][60][61][62][63][64]. A chemiluminescence antibody microarray was developed for the rapid and simultaneous detection of Escherichia coli O157: H7, Salmonella typhimurium, and Legionella pneumophila in water samples using a sensitive CCD camera for signal detection.…”

Section: Biosensorsmentioning

confidence: 99%

Recent developments in rapid multiplexed bioanalytical methods for foodborne pathogenic bacteria detection

et al. 2012

View full text Add to dashboard Cite

Foodborne illnesses caused by pathogenic bacteria represent a widespread and growing problem to public health, and there is an obvious need for rapid detection of food pathogens. Traditional culture-based techniques require tedious sample workup and are time-consuming. It is expected that new and more rapid methods can replace current techniques. To enable large scale screening procedures, new multiplex analytical formats are being developed, and these allow the detection and/or identification of more than one pathogen in a single analytical run, thus cutting assay times and costs. We review here recent advancements in the field of rapid multiplex analytical methods for foodborne pathogenic bacteria. A variety of strategies, such as multiplex polymerase chain reaction assays, microarray-or multichannel-based immunoassays, biosensors, and fingerprint-based approaches (such as mass spectrometry, electronic nose, or vibrational spectroscopic analysis of whole bacterial cells), have been explored. In addition, various technological solutions have been adopted to improve detectability and to eliminate interferences, although in most cases a brief pre-enrichment step is still required. This review also covers the progress, limitations and future challenges of these approaches and emphasizes the advantages of new separative techniques to selectively fractionate bacteria, thus increasing multiplexing capabilities and simplifying sample preparation procedures.

show abstract

Biosensors

Mulaa¹,

Krämer²

2011

Handbook of Food Safety Engineering

View full text Add to dashboard Cite

Rapid, specific and sensitive electrochemical detection of foodborne bacteria

Cited by 60 publications

References 25 publications

Biosensors for the analysis of microbiological and chemical contaminants in food

Biosensors for the analysis of microbiological and chemical contaminants in food

Recent developments in rapid multiplexed bioanalytical methods for foodborne pathogenic bacteria detection

Biosensors

Contact Info

Product

Resources

About