Rapid separation of phosphopeptides by microchip electrophoresis–electrospray ionization mass spectrometry

Ollikainen, Elisa; Bonabi, Ashkan; Nordman, Nina; Jokinen, Ville; Kotiaho, Tapio; Kostiainen, Risto; Sikanen, Tiina

doi:10.1016/j.chroma.2016.02.063

Cited by 17 publications

(11 citation statements)

References 24 publications

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“…Peptide derivatization is sometimes useful also for their MS detection. Derivatization of two monophosphorylated positional isomers of insulin receptor peptide and triply phosphorylated insulin receptor peptide, all with the same amino acid sequence, with 9‐fluorenylmethyl reagents (chloroformate, Fmoc‐Cl, or N‐succinimidyl carbonate, Fmoc‐OSu), allowed efficient separation of phosphorylated peptides from nonphosphorylated peptides by microchip CE using 30 mM ammonium acetate BGE in mixed hydro‐organic 40/40 v/v water/methanol solvent and and on‐chip ESI/MS detection . The derivatization enabled not only the separation of monophosphorylated positional peptide isomers but also identification of the phosphorylation site by tandem MS detection.…”

Section: Sample Preparationmentioning

confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2015–mid 2017)

Kašička

2017

Electrophoresis

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The review brings a comprehensive overview of recent developments and applications of high performance capillary and microchip electroseparation methods (zone electrophoresis, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography) to analysis, microscale isolation, purification, and physicochemical and biochemical characterization of peptides in the years 2015, 2016, and ca. up to the middle of 2017. Advances in the investigation of electromigration properties of peptides and in the methodology of their analysis (sample preseparation, preconcentration and derivatization, adsorption suppression and EOF control, and detection) are described. New developments in particular CE and CEC methods are presented and several types of their applications to peptide analysis are reported: qualitative and quantitative analysis, determination in complex (bio)matrices, monitoring of chemical and enzymatical reactions and physical changes, amino acid, sequence and chiral analysis, and peptide mapping of proteins. Some micropreparative peptide separations are shown and capabilities of CE and CEC methods to provide important physicochemical characteristics of peptides are demonstrated.

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Section: Sample Preparationmentioning

confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2015–mid 2017)

Kašička

2017

Electrophoresis

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“…Microchip electrophoresis (ME) devices can be used in association with different detection modes including optical , mass spectrometry and electrochemical systems . The coupling of ME with electrochemical detection has received considerable attention due to the compatibility with microfabrication technologies and also the possibility to integrate channels and electrodes in a single platform .…”

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confidence: 99%

Authenticity screening of seized whiskey samples using electrophoresis microchips coupled with contactless conductivity detection

et al. 2016

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This report describes for the first time the use of microchip electrophoresis (ME) devices integrated with capacitively coupled contactless conductivity detection (C D) to investigate the authenticity of seized whiskey samples, which were probably adulterated by simple dilution with tap water. The proposed microfluidic platform was explored for the monitoring of anionic species (Cl and F ) in both original and tampered samples. The best separations were achieved within 70 s using a running buffer composed of lactic acid and histidine (pH = 5.9). ME-C D devices were used to analyze samples from three different brands (five samples each). Based on the presence of inorganic anions like Cl , F , SO and NO in different amounts, the authenticity of seized whiskeys was compared to original samples. According to the reported data, the proposed microfluidic platform can be useful to help regulatory authorities in the investigation and monitoring of authenticity of commercialized whiskey beverages.

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“…The microfluidic CE brings wider range of emitter designs. Electrospraying from an attached emitter, corner of glass rectangular chip or integrated emitter was successfully used in the field of separation and MS identification of intact antibodies and in comprehensive proteomics .…”

Section: Development Of Methodology and Instrumentationmentioning

confidence: 99%

Recent advances in CE‐MS coupling: Instrumentation, methodology, and applications

2016

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This review focuses on the latest development of microseparation electromigration methods in capillaries and microfluidic devices coupled with MS for detection and identification of important analytes. It is a continuation of the review article on the same topic by Kleparnik (Electrophoresis 2015, 36, 159-178). A wide selection of 161 relevant articles covers the literature published from June 2014 till May 2016. New improvements in the instrumentation and methodology of MS interfaced with capillary or microfluidic versions of zone electrophoresis, isotachophoresis, and isoelectric focusing are described in detail. The most frequently implemented MS ionization methods include electrospray ionization, matrix-assisted desorption/ionization and inductively coupled plasma ionization. Although the main attention is paid to the development of instrumentation and methodology, representative examples illustrate also applications in the proteomics, glycomics, metabolomics, biomarker research, forensics, pharmacology, food analysis, and single-cell analysis. The combinations of MS with capillary versions of electrochromatography, and micellar electrokinetic chromatography are not included.

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Rapid separation of phosphopeptides by microchip electrophoresis–electrospray ionization mass spectrometry

Cited by 17 publications

References 24 publications

Recent developments in capillary and microchip electroseparations of peptides (2015–mid 2017)

Recent developments in capillary and microchip electroseparations of peptides (2015–mid 2017)

Authenticity screening of seized whiskey samples using electrophoresis microchips coupled with contactless conductivity detection

Recent advances in CE‐MS coupling: Instrumentation, methodology, and applications

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