Rapid separation and determination of structurally related anthraquinones in Rhubarb by pressurized capillary electrochromatography

Huang, Lu; Wang, Jiabin; Wang, Xiaochun; Lin, Xucong; Wu, Xiaoping; Xie, Zenghong

doi:10.1016/j.jpba.2006.06.023

Cited by 49 publications

(13 citation statements)

References 26 publications

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“…[7][8][9][10] However, these methods of extraction are laborious, time consuming, offer low yield and are not suitable for commercial applications. [7][8][9][10] However, these methods of extraction are laborious, time consuming, offer low yield and are not suitable for commercial applications.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of anti-diabetic and alpha glucosidase inhibitory action of anthraquinones from Rheum emodi

Arvindekar

Payghan

et al. 2015

Food Funct.

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Rheum emodi is used as a culinary plant across the world and finds an eminent role in the Ayurvedic and traditional Chinese systems of medicine. The plant is known to principally contain 1,8-dihydroxyanthraquinones (DHAQs) like rhein, aloe emodin, emodin, chrysophanol and physcion that possess diverse pharmacological and therapeutic actions. The present work deals with developing a platform technology for isolation of these DHAQs and evaluating their anti-diabetic potential. Herein, we report the anti-hyperglycemic activity and alpha glucosidase (AG) inhibitory actions of five isolated DHAQs from R. emodi. All the five isolated DHAQs showed good anti-hyperglycemic activity with aloe emodin exhibiting maximum lowering of blood glucose in an oral glucose tolerance test. However, on evaluation of the AG inhibitory potential of the DHAQs only emodin exhibited potent intestinal AG inhibition (93 ± 2.16%) with an IC50 notably lower than acarbose. Subsequent kinetic studies indicated a mixed type of inhibition for emodin. In vivo studies using oral maltose load showed almost total inhibition for emodin when compared to acarbose. Molecular docking studies revealed the presence of an allosteric topographically distinct 'quinone binding site' and showed that interaction with Ser 74 occurs exclusively with emodin, which is vital for AG inhibition. The net benefit from the glucose lowering effect and mixed type inhibition by emodin would enable the administration of a small dosage that is safe and non-toxic in the case of prolonged use in treating diabetes.

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Section: Introductionmentioning

confidence: 99%

Evaluation of anti-diabetic and alpha glucosidase inhibitory action of anthraquinones from Rheum emodi

Arvindekar

Payghan

et al. 2015

Food Funct.

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“…Guo et al [121] prepared BMA-EDMA monolithic column for the determination of cytidine and adenosine in cordyceps. In addition, Xie's group prepared a poly(iso-butyl methacrylate-co-ethylene dimethacrylate-co-methacrylic acid) monolithic column for the rapid separation and determination of anthraquinones in Rhubarb [122] and a poly(glycidyl methacrylate-co-ethylene dimethacrylate co sulfopropyl methacrylate) monolithic column for the determination of five major opium alkaloids in pericarpium papaveris [123].…”

Section: Cec In the Analysis Of Tcmsmentioning

confidence: 99%

Recent Applications of Capillary Electrophoresis in the Analysis of Traditional Chinese Medicines

Feng

Tian²,

Hu³

et al. 2010

CCHTS

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Traditional Chinese medicines (TCMs) are generally mixtures of herbal plants or extracts which comprise hundreds of different constituents with widely difference in the content and physiochemical properties. In order to analyze bioactive compounds in TCMs and control the quality, a large number of analytical tools have been developed, among which capillary electrophoresis (CE) has become a powerful technique with increasing importance. Some formats of CE, including capillary zone electrophoresis, micellar electrokinetic chromatography, non-aqueous CE and capillary electrochromatography have been widely employed for the analysis of TCMs. The general characteristics of these formats are briefly described, and their applications to the analysis of TCMs during the past five years are summarized.

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“…Until now, there are many methods that have been conducted for the determination of anthraquinone compounds, such as capillary zone electrophoresis (CZE) [7], capillary electrochromatography (CE) [8], thin layer chromatography (TLC) [9], and micellar electrokinetic chromatography (MEKC) [10, 11]. However, due to its easy operation, high accuracy, and wide applicability, HPLC is still most widely used among these techniques [12–18].…”

Section: Introductionmentioning

confidence: 99%

“…However, some of these methods are usually time-consuming, solvents, or labor-intensive. Due to its simplicity and low solvent consumption, solid phase extraction (SPE) methods [8, 12, 13] have emerged as effective alternatives for sample preparation method. However, the traditional reversed phase or ion-exchange sorbents are usually showing indiscriminate adsorption effects, resulting in a large number of matrix interferences, which may seriously affect the accuracy of the measurement.…”

Section: Introductionmentioning

confidence: 99%

SPE-UHPLC-FLD Method for the Simultaneous Determination of Five Anthraquinones in Human Urine Using Mixed-Mode Bis(tetraoxacalix[2]arene[2]triazine) Modified Silica as Sorbent

Qiao

Deng

et al. 2017

Journal of Analytical Methods in Chemistry

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The five anthraquinones compounds (including aloe-emodin, emodin, physcion, chrysophanol, and rhein) are regarded as the main effective ingredients in rhubarb (Dahuang in Chinese, one of the commonly used Chinese herbal medicines). In this work, a simple and effective solid phase extraction (SPE) method based on bis(tetraoxacalix[2]arene[2]triazine) modified silica gel as adsorbent was developed. Coupled with UHPLC-FLD, the developed method was successfully applied for the measuring of main anthraquinones in human urine after oral administration of the extracts of rhubarb. To obtain the highest recoveries of the five anthraquinones in the SPE process, the main parameters which may affect extraction efficiency were optimized. The optimized sorbent amount, sample loading pH, sample loading rate, washing solution, and eluent condition were obtained. The developed method showed good linearity in 0.012–1.800 μg mL−1 for the five anthraquinones with correlation coefficients more than 0.9993. The investigated LOD values ranged from 3.9 to 5.7 ng mL−1, while the LOQs were between 12.0 and 18.2 ng mL−1. The recoveries of the method were also investigated, which were in the range of 94.8–106.6%. The application of the mixed-mode SPE materials in the proposed method was feasible and simple, and suitable for the enrichment of anthraquinones in urine samples.

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Rapid separation and determination of structurally related anthraquinones in Rhubarb by pressurized capillary electrochromatography

Cited by 49 publications

References 26 publications

Evaluation of anti-diabetic and alpha glucosidase inhibitory action of anthraquinones from Rheum emodi

Evaluation of anti-diabetic and alpha glucosidase inhibitory action of anthraquinones from Rheum emodi

Recent Applications of Capillary Electrophoresis in the Analysis of Traditional Chinese Medicines

SPE-UHPLC-FLD Method for the Simultaneous Determination of Five Anthraquinones in Human Urine Using Mixed-Mode Bis(tetraoxacalix[2]arene[2]triazine) Modified Silica as Sorbent

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