Rapid self-assembly of submicrospheres at liquid surface by controlling evaporation and its mechanism

Zhang, Labao; Xiong, Yi

doi:10.1016/j.jcis.2006.10.083

Cited by 18 publications

(9 citation statements)

References 18 publications

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“…The order and quality of the assembled arrays are substantially affected by the rates of solvent evaporation. , Control over the temperature and the humidity of the system on a slightly tilted substrate can yield colloidal monolayers . Methods such as spin-coating, Langmuir–Blodgett deposition, and controlled evaporation have been used to assemble large-scale monolayers of colloidal suspensions.…”

mentioning

confidence: 99%

Bench-Top Fabrication of Single-Molecule Nanoarrays by DNA Origami Placement

et al. 2021

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Large-scale nanoarrays of single biomolecules enable high-throughput assays while unmasking the underlying heterogeneity within ensemble populations. Until recently, creating such grids which combine the advantages of microarrays and single-molecule experiments (SMEs) has been particularly challenging due to the mismatch between the size of these molecules and the resolution of top-down fabrication techniques. DNA origami placement (DOP) combines two powerful techniques to address this issue: (i) DNA origami, which provides a ∼100 nm self-assembled template for singlemolecule organization with 5 nm resolution and (ii) top-down lithography, which patterns these DNA nanostructures, transforming them into functional nanodevices via large-scale integration with arbitrary substrates. Presently, this technique relies on state-of-the-art infrastructure and highly trained personnel, making it prohibitively expensive for researchers. Here, we introduce a cleanroom-free, $1 benchtop technique to create meso-to-macro-scale DNA origami nanoarrays using self-assembled colloidal nanoparticles, thereby circumventing the need for top-down fabrication. We report a maximum yield of 74%, 2-fold higher than the statistical limit of 37% imposed on non-specific molecular loading alternatives. Furthermore, we provide a proof-of-principle for the ability of this nanoarray platform to transform traditionally low-throughput, stochastic, single-molecule assays into high-throughput, deterministic ones, without compromising data quality. Our approach has the potential to democratize single-molecule nanoarrays and demonstrates their utility as a tool for biophysical assays and diagnostics.

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confidence: 99%

Bench-Top Fabrication of Single-Molecule Nanoarrays by DNA Origami Placement

et al. 2021

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“…However, the floating monolayer is left free without action of any barrier to modify its assembly, which greatly simplifies the apparatus. Moreover, instead of being slowly withdrawn from the suspension, the substrate is left immersed during the liquid evaporation [68,69].…”

Section: Self-assembly At Interface Of Two Media (I) Air-liquid Intermentioning

confidence: 99%

Nanosphere Lithography: A Powerful Method for the Controlled Manufacturing of Nanomaterials

Colson

Henrist

Cloots

2013

Journal of Nanomaterials

222

164

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The never-ending race towards miniaturization of devices induced an intense research in the manufacturing processes of the components of those devices. However, the complexity of the process combined with high equipment costs makes the conventional lithographic techniques unfavorable for many researchers. Through years, nanosphere lithography (NSL) attracted growing interest due to its compatibility with wafer-scale processes as well as its potential to manufacture a wide variety of homogeneous one-, two-, or three-dimensional nanostructures. This method combines the advantages of both top-down and bottom-up approaches and is based on a two-step process: (1) the preparation of a colloidal crystal mask (CCM) made of nanospheres and (2) the deposition of the desired material through the mask. The mask is then removed and the layer keeps the ordered patterning of the mask interstices. Many groups have been working to improve the quality of the CCMs. Throughout this review, we compare the major deposition techniques to manufacture the CCMs (focusing on 2D polystyrene nanospheres lattices), with respect to their advantages and drawbacks. In traditional NSL, the pattern is usually limited to triangular structures. However, new strategies have been developed to build up more complex architectures and will also be discussed.

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“…Control over the temperature and the humidity of the system on a slightly tilted substrate can yield colloidal monolayers 47 . Methods such as spin-coating 48 , Langmuir-Blodgett deposition 49 , and controlled evaporation 50 have all been used to assemble large-scale monolayers of colloidal suspensions.…”

Section: Introductionmentioning

confidence: 99%

Low-cost, bottom-up fabrication of large-scale single-molecule nanoarrays by DNA origami placement

Shetty

Brady²,

Rothemund

et al. 2020

Preprint

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Large-scale nanoarrays of single biomolecules enable high-throughput assays while unmasking the underlying heterogeneity within ensemble populations. Until recently, creating such grids which combine the unique advantages of microarrays and single-molecule experiments (SMEs) has been particularly challenging due to the mismatch between the size of these molecules and the resolution of top-down fabrication techniques. DNA Origami Placement (DOP) combines two powerful techniques to address this issue: (i) DNA origami, which provides a 100-nm self-assembled template for single-molecule organization with 5 nm resolution, and (ii) top-down lithography, which patterns these DNA nanostructures, transforming them into functional nanodevices via large-scale integration with arbitrary substrates. Presently, this technique relies on state-of-the-art infrastructure and highly-trained personnel, making it prohibitively expensive for researchers. Here, we introduce a bench-top technique to create meso-to-macro-scale DNA origami nanoarrays using self-assembled colloidal nanoparticles, thereby circumventing the need for top-down fabrication. We report a maximum yield of 74%, two-fold higher than the statistical limit of 37% imposed on non-specific molecular loading alternatives. Furthermore, we provide a proof-of-principle for the ability of this nanoarray platform to transform traditionally low-throughput, stochastic, single-molecule assays into high-throughput, deterministic ones, without compromising data quality. Our approach has the potential to democratize single-molecule nanoarrays and demonstrates their utility as a tool for biophysical assays and diagnostics.

show abstract

Rapid self-assembly of submicrospheres at liquid surface by controlling evaporation and its mechanism

Cited by 18 publications

References 18 publications

Bench-Top Fabrication of Single-Molecule Nanoarrays by DNA Origami Placement

Bench-Top Fabrication of Single-Molecule Nanoarrays by DNA Origami Placement

Nanosphere Lithography: A Powerful Method for the Controlled Manufacturing of Nanomaterials

Low-cost, bottom-up fabrication of large-scale single-molecule nanoarrays by DNA origami placement

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