Rapid screening of neuroprotective components from Huang-Lian-Jie-Du Decoction by living cell biospecific extraction coupled with HPLC-Q-Orbitrap-HRMS/MS analysis

Zhang, Zhi-Tong; Huang, Guang-Xiao; He, Wen-Jiao; Gu, Wen-Ting; Wang, Xue; Chen, Zhiquan; Bi, Fujun; Zhang, Luyong; Wang, Shumei; Tang, Dan

doi:10.1016/j.jchromb.2021.122764

Cited by 7 publications

(8 citation statements)

References 32 publications

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“…QTOF‐MS/MS is a high‐resolution MS technique which allows for efficient characterization of compounds in complex natural products 17–19 . After neutral loss/diagnostic ion filtering (extraction width at ± 5 mDa), 12 polymethoxylated flavones and four polymethoxylated flavanones were fished out from the ethanol extract of lysed MCF‐7 cells.…”

Section: Resultsmentioning

confidence: 99%

“…17 Zhang et al utilized a PC12 cell extraction method to specifically screen neuroprotective compounds in Huang-Lian-Jie-Du decoction. 18 Moreover, living cellbased screening methods facilitate high-throughput operation, which will accelerate the discovery of bioactive compounds in natural products.…”

Section: Introductionmentioning

confidence: 99%

“…After living cell biospecific extraction, HPLC-quadrupole time-offlight-MS/MS (HPLC-QTOF-MS/MS) plays an increasingly critical role in the analysis and identification of bioactive compounds. [17][18][19] However, overwhelming amounts of MS datasets are generated, which makes manual data analysis challenging, especially for achieving MS information of low-abundance compounds being overwhelmed by major compounds or background noise. 20 To facilitate and simplify post-acquisition MS data processing, various data mining technologies have been developed with excellent resolution, high sensitivity, outstanding selectivity, and excellent accuracy, such as neutral loss filtering, diagnostic ion filtering, de novo identification, mass defect filtering, isotope pattern filtering, predicted compound screening, and database-assisted peak annotation.…”

Section: Introductionmentioning

confidence: 99%

“…More recently, Hu et al established RBL‐2H3 cell biospecific extract technology to efficiently screen antiallergy compounds in Radix Paeoniae Alba, Radix Astragali, and Radix Saposhnikoviae 17 . Zhang et al utilized a PC12 cell extraction method to specifically screen neuroprotective compounds in Huang‐Lian‐Jie‐Du decoction 18 . Moreover, living cell‐based screening methods facilitate high‐throughput operation, which will accelerate the discovery of bioactive compounds in natural products.…”

Section: Introductionmentioning

confidence: 99%

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Rapid profiling of potential antitumor polymethoxylated flavonoids in natural products by integrating cell biospecific extraction with neutral loss/diagnostic ion filtering‐based high‐performance liquid chromatography–quadrupole time‐of‐flight tandem mass spectrometry

Wang

Cao

et al. 2022

Phytochemical Analysis

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Introduction Citri Reticulatae Pericarpium Viride (CRPV, Qing Pi in Chinese) has been widely used in traditional Chinese medicine. Polymethoxylated flavonoids (PMFs), which are a special group of flavonoids with strong antitumor activity, are broadly distributed in citrus peels. However, systematic investigation of antitumor PMFs in CRPV has received little attention to date. Objectives An MCF‐7 cell biospecific extraction method integrated with neutral loss/diagnostic ion filtering‐based HPLC‐QTOF‐MS/MS strategy was developed for rapid and specific profiling of antitumor PMFs and systematic identification of PMFs in CRPV. Methodology By incubating MCF‐7 cells with CRPV extract, potential antitumor PMFs specifically bound to cells and were isolated. Then, by systematic investigation of fragmentation pathways, neutral loss and diagnostic ion filtering strategies were proposed to comprehensively and accurately identify PMFs. Results Sixteen antitumor PMFs were unambiguously or tentatively identified. Among them, minor compound 15 (5‐hydroxy‐6,7,8,3′,4′‐pentamethoxyflavone with a free hydroxyl group at C‐5) exhibited excellent antitumor activity, with an IC50 value of 2.81 ± 0.76 μg/mL, which is lower than that of 5‐fluorouracil (IC50, 4.92 ± 0.83 μg/mL). Nobiletin (12) and tangeretin (16), two major PMFs, presented moderate antitumor activities with IC50 values of 13.06 ± 1.85 and 17.07 ± 1.18 μg/mL, respectively, and their contents were sensitively and precisely determined. Conclusions To the best of our knowledge, this is the first report on the systematic investigation of antitumor PMFs in CRPV. The study will lay a foundation for the quality control and clinical application of CRPV.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Rapid profiling of potential antitumor polymethoxylated flavonoids in natural products by integrating cell biospecific extraction with neutral loss/diagnostic ion filtering‐based high‐performance liquid chromatography–quadrupole time‐of‐flight tandem mass spectrometry

Wang

Cao

et al. 2022

Phytochemical Analysis

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“…The conventional method for studying bioactive components was burdensome, since there may be hundreds, even thousands of compounds to isolate, purify, and evaluate the bioactivity. Recently, the application of living cell extraction with HPLC-MS for screening the potential components has attracted much attention [ 37 – 39 ]. The cell extraction method was simple and reliable to screen the bioactive substance.…”

Section: Discussionmentioning

confidence: 99%

Erythrocyte Storage Lesion Improvements Mediated by Naringin Screened from Vegetable/Fruit Juice Using Cell Extract and HPLC-MS

She

Liu

Xiong

et al. 2022

Journal of Analytical Methods in Chemistry

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In blood banking, storage at 4°C for weeks is known to cause damages to erythrocytes, called storage lesions that may later cause transfusion-related adverse events. In previous experiments, we found that vegetable/fruit juices can effectively reduce the storage lesion. Currently, we attempt to analyze the potential bioactive components and test whether the compounds can improve the storage lesions of erythrocytes. Equal portions in wet weight of 20 fresh vegetables and fruits were blended with phosphate buffered solution (PBS), and clear solutions were produced as additive to the packed erythrocytes from consented blood donors at 1 : 10 ratio (ml : gram). The blood samples were stored for 35 days at 4°C, and the supernatants were performed high liquid chromatography–mass spectrometry (HPLC-MS) analysis at 0 days, 14 days, and 35 days. The blood bags supplemented with identified bioactive components were stored in a refrigerator for 35 days, and the morphology, complete blood count (CBC), phosphatidylserine (PS) extroversion, hemolysis, and reactive oxygen species (ROS) levels were measured at the end of storage. Five potential bioactive components from vegetable/fruit juices contributed to the improvements of storage lesion. One of the compounds was unequivocally identified as naringin, and two were tentatively assigned as vitexin 6″-O-malonyl 2″-O-xyloside and luteolin 7-(6″-malonyl neohesperidoside). Naringin alleviated the storage lesion of red blood cells (RBCs) by reducing ROS levels and living cell extraction with HPLC-MS is a simple, reliable, and effective method for screening potential bioactive components.

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Combination of UHPLC‐Q Exactive‐Orbitrap MS, Bioinformatics and Molecular Docking to Reveal the Mechanism of Huan‐Lian‐Jie‐Du Decoction in the Treatment of Diabetic Encephalopathy

Cao

Liang

Zhang

et al. 2023

Chemistry & Biodiversity

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Diabetic encephalopathy (DE) is a serious complication of diabetes, which affects patients′ quality of life. We aimed to explore HLJDD in the treatment of DE by LC/MS and bioinformatics. UPLC‐Q Exactive‐Orbitrap MS was employed to clarify the compounds. The modules and hub targets of DE were gained from WGCNA. Subsequently, an Herb‐Compound‐Target network was constructed and enrichment analysis was used. In addition, a protein‐protein interaction (PPI) network was constructed and molecular docking was used to verify the above analysis. As result, 138 compounds and 10 prototypes in brain were identified. In network pharmacology, 8 modules and 5692 hub targets were obtained from WGCNA. An Herb‐Compound‐Target network was constructed by 4 herbs, 10 compounds and 56 targets. The enrichment analysis showed that the treatment of DE with HLJDD involve oxidative stress and neuroprotection. Beside, SRC, JUN, STAT3, MAPK1 and PIK3R1 were identified and as hub targets of HLJDD in treating DE. Moreover, Molecular docking showed that five hub targets had strong affinity with the corresponding alkaloids. Therefore, we explored the underlying mechanisms of HLJDD in the treatment of DE and to provide the theoretical and scientific basis for subsequent experimental studies and clinical applications.

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Rapid screening of neuroprotective components from Huang-Lian-Jie-Du Decoction by living cell biospecific extraction coupled with HPLC-Q-Orbitrap-HRMS/MS analysis

Cited by 7 publications

References 32 publications

Rapid profiling of potential antitumor polymethoxylated flavonoids in natural products by integrating cell biospecific extraction with neutral loss/diagnostic ion filtering‐based high‐performance liquid chromatography–quadrupole time‐of‐flight tandem mass spectrometry

Rapid profiling of potential antitumor polymethoxylated flavonoids in natural products by integrating cell biospecific extraction with neutral loss/diagnostic ion filtering‐based high‐performance liquid chromatography–quadrupole time‐of‐flight tandem mass spectrometry

Erythrocyte Storage Lesion Improvements Mediated by Naringin Screened from Vegetable/Fruit Juice Using Cell Extract and HPLC-MS

Combination of UHPLC‐Q Exactive‐Orbitrap MS, Bioinformatics and Molecular Docking to Reveal the Mechanism of Huan‐Lian‐Jie‐Du Decoction in the Treatment of Diabetic Encephalopathy

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