Rapid Screening and Identification of BSA Bound Ligands from Radix astragali Using BSA Immobilized Magnetic Nanoparticles Coupled with HPLC-MS

Abstract

Radix astragali is widely used either as a single herb or as a collection of herbs in a complex prescription in China. In this study, bovine serum albumin functionalized magnetic nanoparticles (BSA-MN) coupled with high performance liquid chromatography-mass spectrometry (HPLC-MS) were used to screen and identify bound ligands from the n-butanol part of a Radix astragali extract. The prepared BSA-MN showed sufficient magnetic response for the separation with an ordinary magnet and satisfied reusability. Fundamental parameters affecting the preparation of BSA-MN and the screening efficiency were studied and optimized. Under the optimum conditions, four bound ligands were screened out from the n-butanol part of a Radix astragali extract and identified as genistin (1), calycosin-7-O-β-D-glucoside (2), ononin (3) and formononetin (4). This effective method could be widely applied for rapid screening and identification of active compounds from complex mixtures without the need for preparative isolation.

Metabolomics combined with network pharmacology exploration reveals the modulatory properties of Astragali Radix extract in the treatment of liver fibrosis

“…var. mongholicus (Bge) Hsiao [5, 6]. In traditional Chinese formula, AR is often used for the treatment of liver fibrosis [6, 7].…”

“…mongholicus (Bge) Hsiao [5, 6]. In traditional Chinese formula, AR is often used for the treatment of liver fibrosis [6, 7]. Fundamental studies have exhibited the anti-hepatic fibrosis effects of AR by inhibiting TGF-β/Smads signaling [8, 9].…”

Analysis of Xanthine Oxidase Inhibitors from Clerodendranthus spicatus with Xanthine Oxidase Immobilized Silica Coated Fe3O4 Nanoparticles

“…After the amino functionalization, the FT-IR spectrum of amino Fe 3 O 4 @SiO 2 nanoparticles showed all of the peaks mentioned above. Moreover, the enhanced wide peak at 3400 cm −1 could be attributed to the N-H stretching vibration [23]. These results indicated that the silica coating and the following amino functionalization on the surface of Fe 3 O 4 nanoparticles were successful.…”

“…To confirm the silica coating and amino groups on the surface of Fe3O4 nanoparticles, FT-IR analysis was also performed. As shown in Figure 3a, the FT-IR spectrum of Fe3O4 nanoparticles showed a characteristic Fe-O vibration band at 588 cm −1 [23]. Figure 3b shows the FT-IR spectrum of Fe3O4@SiO2 nanoparticles.…”

Comprehensive Identification of Guan-Xin-Shu-Tong Capsule via a Mass Defect and Fragment Filtering Approach by High Resolution Mass Spectrometry: In Vitro and In Vivo Study

“…The reported identification analysis of Salvia miltiorrhiza Bunge only included nuclear magnetic resonance spectroscopy, high performance liquid chromatography and liquid chromatography quadrupole-time-of-flight mass spectrometry (LC-Q-TOF/MS) [ 20 , 21 , 22 , 23 ]. Currently, LC-Q-TOF/MS has been applied widely in the separation and identification of complex components in TCM [ 30 , 34 , 35 ]. Compared to other mass spectrometry methods, Q-TOF/MS has the characteristics of greater accuracy, higher scanning speed and a wider range of masses [ 36 ].…”