Metabolomics combined with network pharmacology exploration reveals the modulatory properties of Astragali Radix extract in the treatment of liver fibrosis

Wang, Dan; Li, Ruisheng; Wei, Shizhang; Gao, Sijia; Xu, Zhuo; Liu, Honghong; Li, Haotian; Cai, Huadan; Wang, Jian; Yan, Zhao

doi:10.1186/s13020-019-0251-z

Cited by 35 publications

(25 citation statements)

References 51 publications

(60 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The data ltering and normalization were accomplished by the MetaboAnalyst 4.0 online software (http://www.metaboanalyst.ca), and then introduce the resultant data matrix into SIMCA-P software (version 14.1; Umetrics, Umea, Sweden) for multivariate analysis including principal component analysis (PCA) and orthogonal projection to latent structures discriminate analysis (OPLS-DA). The differential metabolites satisfying the conditions (VIP > 1 and |P(corr)| ≥ 0.58) in the OPLS-DA analysis were selected as potential biomarkers [13] .…”

Section: Data Processing and Multivariate Analysismentioning

confidence: 99%

Study on the Protective Effect and the Metabolomics Features of Rutaecarpine on Ethanol-induced Acute Gastric Ulcer

Ren

Wei

Niu

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

As a prevalent digestive disease, Gastric ulcer (GU) has a high incidence and is seriously harmful to human health. It is an urgent need to finding a natural drug with a gastroprotective effect. Rutaecarpine (RUT) is an alkaloid isolated from Evodia rutaecarpa Bentham (Rutaceae). This plant is a traditional Chinese medicine that has been treating gastrointestinal diseases for a long time. The present study aimed to investigate the effects of RUT on ethanol-induced acute GU in mice. After intragastrical administration of RUT for 3 consecutive days, acute GU were induced in the mice by ethanol treatment for 1.5 h. The expression levels of SOD, CAT, NO, ET-1 in serum and EGF, TNF-α, IL-1β, IL-6 in gastric tissues were measured by kits. In addition, hematoxylin and eosin (H&E) staining of gastric mucosa for pathological changes was evaluated. The serum metabolomics method based on ultra-high-performance liquid chromatography combined with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) was used to explore the potential mechanism. A total of 7 potential metabolites involved in 9 metabolic pathways were identified. This study helps us to further understand the pathogenesis of GU and provide a potential natural anti-ulcer drug for clinic.

show abstract

Section: Data Processing and Multivariate Analysismentioning

confidence: 99%

Study on the Protective Effect and the Metabolomics Features of Rutaecarpine on Ethanol-induced Acute Gastric Ulcer

Ren

Wei

Niu

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…Then, 3,3′-diaminobenzidine (DAB) chromogen substrate solution was utilized to visualize the results. 30) UPLC/Q-TOF-MS Analysis Conditions Chromatographic Conditions HPLC-Q-TOF-MS system was used for serum metabolic spectrum analysis. One microliter aliquot of each sample was injected into the system on a HSS T3 column analytical column (2.1 × 100 mm, 1.8 µm, waters) for sample separation at 45°C.…”

Section: Ultra-performance (Up) Lc/quadrupole Time-of-flight (Q-tof)-mentioning

confidence: 99%

“…Samples Quality Control Assessment QC can determine whether the systematic error of the whole experiment is within the controllable range. 31) In this study, QC samples and experimental samples were analyzed using unsupervised PCA. PCA scores of ESI positive ion mode, negative ion mode and HILIC mode are shown (Figs.…”

Section: Levels Of Serum Parameters and Histopathology Testmentioning

confidence: 99%

A Serum Metabolomic Study on Rats Induced by Polygoni Multiflori Radix and Polygoni Multiflori Radix Preparata by Pattern Recognition and Pathways Analysis

Zhang

Huang

Gao

2020

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

This study focused on the differential metabolomic effects between water extracts of Polygoni Multiflori Radix and Polygoni Multiflori Radix Preparata in rats. The extracts were subsequently administered for 28 d. Serum biochemical indicators were tested, hematoxylin-eosin staining and immunohistochemistry staining were used to detect histopathological changes in the livers. Ultra-performance LC/quadrupole timeof-flight mass spectrometry was used to detect the changes in endogenous metabolites. Finally, we performed detailed analysis of the changes in metabolic pathways. Hematoxylin-eosin staining and immunohistochemistry staining results indicated that the water extracts of Polygoni Multiflori Radix and Polygoni Multiflori Radix Preparata had mild liver injury effect. Fifty-two differential endogenous biomarkers were confirmed as potential biomarkers between Polygoni Multiflori Radix and Polygoni Multiflori Radix Preparata groups. In the positive ion mode, the biomarkers included 31 Phosphatidyl cholines (PCs), six lysoPCs, and ceramide. In the negative ion mode, 12 biomarkers were confirmed, including glycodeoxycholic acid, chenodeoxycholic acid, and deoxycholic acid, etc. In Hydrophilic Interaction Liquid Chromatography (HILIC) mode, nine biomarkers were confirmed, including niacinamide, L-palmitoylcarnitine, and butyrylcarnitine, etc. Using MetaboAnalyst 4.0, six related metabolic pathways, including taurine and hypotaurine metabolism, sphingolipid metabolism, glycerophospholipid metabolism, nicotinate and nicotinamide metabolism, arginine and proline metabolism, and tryptophan metabolism and primary bile synthesis, were confirmed as the most differential pathways between the Polygoni Multiflori Radix and Polygoni Multiflori Radix Preparata groups.

show abstract

“…Network pharmacology is an emerging discipline that builds and analyzes biological networks based on systems biology to reveal the role of drugs and their mechanisms [13,14]. By constructing a "componenttarget-pathway" research network, we can observe how drugs act on multiple targets at the same time, thereby regulating multiple signal pathways, comprehensively reveal their drug e cacy network, and explain their mechanism of action [15].…”

Section: Introductionmentioning

confidence: 99%

Anemoside B4 protects against Klebsiella pneumoniae- and influenza virus FM1-induced pneumonia via the TLR4/Myd88 signaling pathway in mice

Yuan

Cui

et al. 2020

Preprint

View full text Add to dashboard Cite

Abstract Background: Pneumonia refers to the inflammation of the terminal airway, alveoli and pulmonary interstitium, which can be caused by pathogenic microorganisms, physical and chemical factors, immune damage, and drugs. Anemoside B4, the major ingredient of Pulsatilla chinensis (Bunge) Regel, exhibited anti-inflammatory activity. However, the therapeutic effect of anemoside B4 on pneumonia has not been unraveled. This study aims to investigate that anemoside B4 attenuates the inflammatory responses in Klebsiella pneumonia (KP)- and influenza virus FM1 (FM1)-induced pneumonia mice model.Methods: The network pharmacology and molecular docking assays were employed to predict the targets of anemoside B4’s treatment of pneumonia. Two models (bacterial KP-infected mice and virus FM1-infected mice) were employed in our study. BALB/c mice were divided into six groups: control, model group (KP- induced pneumonia or FM1-induced pneumonia), anemoside B4 (B4)-treated group (2.5, 5, 10 mg/kg), and positive drug group (Ribavirin or Ceftriaxone Sodium Injection). Blood samples were collected for hematology analysis. The effects of B4 on inflammation-associated mediators were investigated by Enzyme-linked immunosorbent assay (ELISA) and hematoxylin and eosin staining (HE) staining. Proteins expression was quantified by western blotting.Results: The network results indicated that many pro-inflammatory cytokines such as tumor necrosis factor α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6) participated in anemoside B4’s anti-inflammatory activity. The counts of neutrophil (NEU) and white blood cell (WBC), the level of myeloperoxidase (MPO), and the release of pro-inflammatory cytokines TNF-α, IL-1β, and IL-6 increased by KP or FM1 infection, which were reversed by anemoside B4. In addition, anemoside B4 significantly suppressed the FM1-induced expression of Toll-like receptor 4 (TLR4), myeloid differential protein-88 (MyD88), and myeloid differentiation protein-2 (MD-2), which were further validated by molecular docking data that anemoside B4 bound to bioactive sites of TLR4. Therefore, anemoside B4 exhibited a significant therapeutic effect on pneumonia via the TLR4/MyD88 pathway.Conclusion: Our findings demonstrated that anemoside B4 attenuates pneumonia via the TLR4/Myd88 signaling pathway, suggesting that anemoside B4 is a promising therapeutic candidate for bacterial-infected or viral-infected pneumonia.

show abstract

Metabolomics combined with network pharmacology exploration reveals the modulatory properties of Astragali Radix extract in the treatment of liver fibrosis

Cited by 35 publications

References 51 publications

Study on the Protective Effect and the Metabolomics Features of Rutaecarpine on Ethanol-induced Acute Gastric Ulcer

Study on the Protective Effect and the Metabolomics Features of Rutaecarpine on Ethanol-induced Acute Gastric Ulcer

A Serum Metabolomic Study on Rats Induced by Polygoni Multiflori Radix and Polygoni Multiflori Radix Preparata by Pattern Recognition and Pathways Analysis

Anemoside B4 protects against Klebsiella pneumoniae- and influenza virus FM1-induced pneumonia via the TLR4/Myd88 signaling pathway in mice

Contact Info

Product

Resources

About