Rapid saccharification for production of cellulosic biofuels

Lee, Dae-Seok; Wi, Seung Gon; Lee, Soo Jung; Lee, Yoon-Gyo; Kim, Yeong-Suk; Bae, Hyeun‐Jong

doi:10.1016/j.biortech.2014.02.039

Cited by 22 publications

(18 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…More recently, various combinations of sodium hydroxide and lime were used to improve ruminal degradability of date palm leaf; under the optimum processing conditions [71.4 h, 40°C, 0.6% (w/w) NaOH and 0.9% (w/w) Ca (OH) 2 ], gas production at 24 h was 2.46-fold over the untreated biomass (Rajaee Rad et al 2014). Enhanced ruminal degradability of the pre-treated SCB is most likely due to its lignin removal, since lignin, a highly oxygenated complex aromatic polymer which firmly complexes cellulose fibres (Lee et al 2014), is an important impediment affecting the rate and extent of rumen digestion of fibre (Waghorn & McNabb 2003). By mitigating this barrier (Table 3), the accessibility of ruminal microorganisms to carbohydrate portion of the biomass may enhance, which consequently leads to an improved rumen degradability.…”

Section: Discussionmentioning

confidence: 99%

Pre-treatment of sugarcane bagasse with a combination of sodium hydroxide and lime for improving the ruminal degradability: optimization of process parameters using response surface methodology

Ahmadi

Zamiri

Khorvash

et al. 2015

Journal of Applied Animal Research

View full text Add to dashboard Cite

A three-factor, three-level, face-centred cubic design was adopted to investigate the effect of three pre-treatment parameters, namely sodium hydroxide-to-dry biomass ratio [0.00, 0.05, and 0.10 (w/w)], lime-to-dry biomass ratio [0.00, 0.05, and 0.10 (w/w)] and residence reaction time (24, 96, and 168 h), at moderate temperature (21°C) on 24-h cumulative gas production (GP 24 ) and delignification, as the response variables. Under the optimal pre-treatment conditions of NaOH-to-dry biomass ratio of 0.09 (w/w), lime-to-dry biomass ratio of 0.09 (w/w) and residence time of 160.3 h, the model predicted 151.6 mL gas/g organic matter (OM) versus the experimental value of 147.2 mL gas/g OM. Under the optimal conditions of NaOH-to-dry biomass ratio of 0.10 (w/w), lime-to-dry biomass ratio of 0.08 (w/w) and residence time of 156.8 h, sugarcane bagasse (SCB) was maximally delignified by 41.2%, whereas the model predicted 43.6% delignification. Under the optimal conditions determined for GP 24 , the neutral detergent fibre degradability of the pre-treated biomass after 24 and 48 h was 37.2 and 56.3%, respectively, a 1.90-fold and 1.58-fold over the untreated biomass, respectively. Overall, it was found that combined alkali treatment at optimal conditions effectively enhanced the ruminal degradability of SCB.

show abstract

Section: Discussionmentioning

confidence: 99%

Pre-treatment of sugarcane bagasse with a combination of sodium hydroxide and lime for improving the ruminal degradability: optimization of process parameters using response surface methodology

Ahmadi

Zamiri

Khorvash

et al. 2015

Journal of Applied Animal Research

View full text Add to dashboard Cite

show abstract

“…T. reesei produces XYN I and XYN II as two main xylanases types in addition to XYN III, and XYN IV. However, zymogram analysis showed that a single 55 kDa band corresponding to XYN IV showed enzymatic activity on birchwood and beechwood xylans [29,30]. Tenkanen et al (2012) previously reported that XYN IV showed a typical exo-action on linear β-1,4-xylooligosaccharides [30], and low activity on xylo-biose, -triose, and -tetraose.…”

Section: Rapid Hydrolyzation Of Softwoodmentioning

confidence: 99%

“…The bers, tracheids, medullary rays, and ray parenchyma cells of HPAC-pretreated oak were separated by ltration with 60 and 100 mesh (S1020, Sigma-Aldrich). HPAC-pretreated pine tracheids were separated, and the tissue fractions (0.5%, g/v) were hydrolyzed in 0.1 M citrate buffer (pH 5.0) containing 15 FPU cellulase at 50 °C for 6 h. Nelson-Somogyi (NS) and DNS assays were used to measure the concentration of the reducing sugars [29].…”

Section: Preparation Of Deligni Ed Bers and Xylem Tissues Fractionmentioning

confidence: 99%

HPAC Pretreatment Facilitates Enzymatic Hydrolysis of Softwood

Lee

Cho

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

Background: Woody plants with high glucose content are alternative bioresources for the production of biofuels and biochemicals. Various pretreatment methods may be used to reduce the effects of retardation factors such as lignin interference and cellulose structural recalcitrance on the degradation of the lignocellulose material of woody plants. Results: Here, a hydrogen peroxide-acetic acid (HPAC) pretreatment was used to reduce the lignin content of several types of woody plants, and the effect of the cellulose structural recalcitrance on the enzymatic hydrolysis was analyzed. The cellulose structural recalcitrance and the degradation patterns of the wood fibers in the xylem tissues of Quercus acutissima (hardwood) resulted in greater retardation in the enzymatic saccharification than those in the tracheids of Pinus densiflora (softwood). In addition to the HPAC pretreatment, application of supplementary enzymes (7.5 FPU cellulose for 24 hours) further increased the hydrolysis rate of P. densiflora from 61.42% to 91.94% whereas the same effect was not observed for Q. acutissima. Also, it was observed that endoxylanase synergism significantly affects the hydrolysis of P. densiflora. However, this synergistic effect was lower for other supplementary enzymes. The maximum concentration of the reducing sugars produced from 10% softwood was 89.17 g L-1 in 36 hours of hydrolysis with 15 FPU cellulase and other supplementary enzymes. Approximately 80 mg mL-1 of reducing sugars was produced with the addition of 7.5 FPU cellulase and other supplementary enzymes after 36hours, achieving rapid saccharification. Conclusion: HPAC pretreatment thus removed the interference of lignin, reduced structural recalcitrance of cellulose in the P. densiflora, and thereby enabled rapid saccharification of the woody plants including a high concentration of insoluble substrates with only low amounts of cellulase. HPAC pretreatment may thus be a viable as an alternative for the cost-efficient production of biofuels or biochemicals from softwood plant tissues.

show abstract

“…The pellet was repeatedly hydrolyzed and washed under the aforementioned conditions until the 6th stage, and then additional hydrolysis was performed for 2 d to nearly completely remove the carbohydrates from the pellet. The concentration of the reducing sugars was measured using a DNS solution (1% dinitrosalicylic acid, 0.05% sodium sulfite, 2% Rochelle salt, 0.2% phenol, 2% NaOH), via a method reported by Lee et al (2014). The initial rate was calculated using the following equation:…”

Section: Enzymatic Rehydrolysismentioning

confidence: 99%

“…PA treatment can be utilized to examine the inhibitory effect of the cellulose structure in the presence of lignin. The cellulose properties were analyzed using Fourier transform infrared spectroscopy after PA or sodium hydroxide treatment, and an increase in the peak absorbance between 3,261 and 3,334 cm −1 indicated the conversion of cellulose type I to type II; the modified type II cellulose was readily hydrolyzed (Oh et al, 2005;Lee et al, 2014).…”

Section: Pretreatment and Comparison Of Cellulose Modificationmentioning

confidence: 99%

Hydrolysis Patterns of Xylem Tissues of Hardwood Pretreated With Acetic Acid and Hydrogen Peroxide

Lee

Song

et al. 2020

Front. Energy Res.

Self Cite

View full text Add to dashboard Cite

The structural recalcitrance of lignocellulose limits its enzymatic hydrolysis, which leads to inefficient enzyme usage and inhibition of saccharification, depending on the pretreatment method. Research on the structural properties of xylem tissues of hardwood and their effect on enzymatic saccharification is necessary to achieve cost-effective biofuel production via improved enzyme cocktail preparation. Oak wood (Quercus acutissima) was pretreated and delignified with a hydrogen peroxide-acetic acid (HPAC) solution. Cellulose was found to undergo significant swelling in the lumen of the wood fiber, and it was sorted into readily hydrolysable (72.9%), mid-hydrolysable (8.2%), and hardly hydrolysable (18.9%) cellulose forms. Oak wood has been shown to be strongly retarded among the various types of hardwoods. The recalcitrance of the xylem tissues, such as wood fibers, tracheids, vessel elements, and ray parenchyma cells, was determined through analysis of the hydrolysis rates. It was found to increase in the following order: ray parenchyma cells < tracheids < wood fibers or vessel elements < tracheids < wood fibers. The wood fibers were almost enzymatically fragmented into pieces ∼90 µm in length at crack sites in 6 h. The wood fibers were digested faster in the S 3 or S 2 wall than in the primary wall. The result indicated that the primary wall may be a structural retardation factor in the hardwood as sorted to the hardly hydrolysable cellulose. In presence of 10% substrate supplemented with enzymes to reduce the structural recalcitrance (xylanase and lytic polysaccharide monoxygenase) and end-product inhibitions (beta-glucosidase), the hydrolysis rate was increased by 55.21%. Ethanol fermentation exhibited a higher efficiency when a single substrate (Q. acutissima) rather than a mixture of various hardwoods was used. Of all the xylem tissues of hardwood that were delignified by HPAC pretreatment, wood fiber was found to be a structural retardation factor owing to the recalcitrance its primary wall. Thus, enzyme preparation can enable the rapid and efficient hydrolysis for the commercialization of bioethanol from hardwood.

show abstract

Rapid saccharification for production of cellulosic biofuels

Cited by 22 publications

References 31 publications

Pre-treatment of sugarcane bagasse with a combination of sodium hydroxide and lime for improving the ruminal degradability: optimization of process parameters using response surface methodology

Pre-treatment of sugarcane bagasse with a combination of sodium hydroxide and lime for improving the ruminal degradability: optimization of process parameters using response surface methodology

HPAC Pretreatment Facilitates Enzymatic Hydrolysis of Softwood

Hydrolysis Patterns of Xylem Tissues of Hardwood Pretreated With Acetic Acid and Hydrogen Peroxide

Contact Info

Product

Resources

About