Rapid Reversed-Phase Chromatographic Method for Determination of Eight Vitamin E Isomers and γ-Oryzanols in Rice Bran and Rice Bran Oil

Shammugasamy, Balakrishnan; Ramakrishnan, Yogeshini; Manan, F.; Muhammad, Kharidah

doi:10.1007/s12161-014-9929-0

Cited by 20 publications

(17 citation statements)

References 23 publications

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“…The fluorescence detection wavelengths were set at Ex/Em = 295/330 nm to detect the tocol compounds, whereas the DAD wavelength was set at 330 nm to detect γ-oryzanol. The column was conditioned using the mobile phase for at least 30 min until a linear baseline was obtained prior to chromatography [24].…”

Section: Determination Of the Tocopherol Tocotrienol And Oryzanol Comentioning

confidence: 99%

Effect of Subcritical Carbon Dioxide Extraction and Bran Stabilization Methods on Rice Bran Oil

Chia

Boo

Muhamad

et al. 2015

J Americ Oil Chem Soc

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Section: Determination Of the Tocopherol Tocotrienol And Oryzanol Comentioning

confidence: 99%

Effect of Subcritical Carbon Dioxide Extraction and Bran Stabilization Methods on Rice Bran Oil

Chia

Boo

Muhamad

et al. 2015

J Americ Oil Chem Soc

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“…In addition, tocopherol-rich oils, such as germ oils from oat [63], barley [64] and wheat [65], may be mixed with other oils to stabilize them [61]. Among the tocotrienol-rich oils, palm oil [28], annatto [66], and rice bran oil [67] are important, and these are often used in product enrichment. Considerable amounts of tocols are removed during the refining process of edible oils.…”

Section: Current and Potential Applications Of Tocopherols And Tocmentioning

confidence: 99%

Tocopherols and Tocotrienols in Common and Emerging Dietary Sources: Occurrence, Applications, and Health Benefits

Shahidi

Camargo

2016

IJMS

321

182

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Edible oils are the major natural dietary sources of tocopherols and tocotrienols, collectively known as tocols. Plant foods with low lipid content usually have negligible quantities of tocols. However, seeds and other plant food processing by-products may serve as alternative sources of edible oils with considerable contents of tocopherols and tocotrienols. Tocopherols are among the most important lipid-soluble antioxidants in food as well as in human and animal tissues. Tocopherols are found in lipid-rich regions of cells (e.g., mitochondrial membranes), fat depots, and lipoproteins such as low-density lipoprotein cholesterol. Their health benefits may also be explained by regulation of gene expression, signal transduction, and modulation of cell functions. Potential health benefits of tocols include prevention of certain types of cancer, heart disease, and other chronic ailments. Although deficiencies of tocopherol are uncommon, a continuous intake from common and novel dietary sources of tocopherols and tocotrienols is advantageous. Thus, this contribution will focus on the relevant literature on common and emerging edible oils as a source of tocols. Potential application and health effects as well as the impact of new cultivars as sources of edible oils and their processing discards are presented. Future trends and drawbacks are also briefly covered.

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“…Although there is no standardised method for the separation and quantification of GO, the compounds are usually performed by HPLC with a posterior compound identification by HPLC-MS or GC-MS (Shammugasamy et al, 2015). Several other methods have been described for the GO analysis in rice, bran or rice oil, such as ultraviolet (UV) spectrophotometry (Bucci et al, 2003), normal phase highperformance liquid chromatography (NP-HPLC) (Lerma-García et al, 2009), reverse phase (RP-HPLC) (Rogers et al, 1993), and gas chromatography (GC) (Miller and Engel, 2006).…”

Section: Introductionmentioning

confidence: 99%

Assessment of Gamma Oryzanol Variability, an Attractive Rice Bran Bioactive Compound

et al. 2020

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Gamma-oryzanol is a compound present on rice bran, which has been studied for its bioactive properties, including its cytotoxic activity. The gamma-oryzanol biosynthesis occurs on the lipidic membrane of the plant and can be influenced by edaphoclimatic grown conditions. Two Portuguese rice varieties (Ceres and Maçarico) from 6 growing environments were analysed in order to explore the influence of the growing conditions on the gamma-oryzanol content, its constituents, and cytotoxic effect against 4 human tumour cell lines (NCI-H460, HeLa, HepG2, and MCF -7). The overall variability of gamma-oryzanol concentration was from 1.56 g / Kg to 3.19 g / Kg, Ceres reveals higher values than Maçarico; those concentrations also varied with the growing environment. The environment was also determinant for the individual concentration of the 3 gamma-oryzanol compounds (cycloartenyl ferulate, 24-methylenecycloartanyl ferulate, and campesteryl ferulate). A cytotoxic effect was observed for all the tested tumour cell lines and also varied with the growing environment.

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Rapid Reversed-Phase Chromatographic Method for Determination of Eight Vitamin E Isomers and γ-Oryzanols in Rice Bran and Rice Bran Oil

Cited by 20 publications

References 23 publications

Effect of Subcritical Carbon Dioxide Extraction and Bran Stabilization Methods on Rice Bran Oil

Effect of Subcritical Carbon Dioxide Extraction and Bran Stabilization Methods on Rice Bran Oil

Tocopherols and Tocotrienols in Common and Emerging Dietary Sources: Occurrence, Applications, and Health Benefits

Assessment of Gamma Oryzanol Variability, an Attractive Rice Bran Bioactive Compound

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