Rapid resolution of drugs and related substances with an eCAPTM polyamine coated capillary

This review deals with the determination of impurities in pharmaceuticals by electromigration methods in the capillary format. These separation methods are either based on the different effective mobility of the charged analytes (as in zone electrophoresis and isotachophoresis) or include hybrid methods such as micellar electrokinetic chromatography, microemulsion electrokinetic chromatography and electrochromatography. The pharmaceutically active compounds under consideration belong to chemotherapeutic agents, central nervous system drugs, histamine receptor drugs, cardiovascular drugs, anticancer drugs, anti-inflammatory drugs and some other drugs. The review discusses about 150 publications from the period between 1980 and 2007 with special emphasis on the recent trends and gives details about the experimental conditions applied for analyses and the obtained analytical performance parameters.

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“…With the minimum possible length of the capillary, the analysis time was reduced to less than 2 min [32].…”

Section: Chemotherapeutic Agentsmentioning

confidence: 99%

Impurity analysis of pharmaceuticals using capillary electromigration methods

Jouyban

Kenndler

2008

Electrophoresis

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“…The m EOF values measured after each of the MMT coatings did not show any significant difference, indicating that the maximum coverage is reached after the first layer of clay is applied (m EOF layer 1 = 1. 7 Different poly(amines) have been used for m EOF reversal [11][12][13][14][15][16]. Therefore, the possibility of modulating the m EOF by selecting different cationic polyelectrolytes was also investigated.…”

Section: Characterization Of the Pdda-mmt-coated Capillariesmentioning

confidence: 99%

“…Cationic coatings have several advantages such as reducing or neutralizing the m EOF and providing a positively charged surface for anchoring other coatings. Amines such as polyamine [11], poly(ethyleneimine) [12,13], Polybrene [14], and poly(diallyldimethyl ammonium chloride) (PDDA) [15,16] have been used to alter the m EOF and improve separations in CE. PDDA has been also used in combination with anionic polymers, to form polyelectrolyte multilayers on silica capillaries [17].…”

Section: Introductionmentioning

confidence: 99%

Electrophoretic separation of environmentally important phenolic compounds using montomorillonite‐coated fused‐silica capillaries

Mora¹,

Garcı́a²

2007

Electrophoresis

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This paper reports a simple procedure for coating fused-silica capillaries with poly(diallyldimethyl ammonium chloride) and montmorillonite. The coated capillaries were characterized by performing EOF measurements as a function of buffer pH, number of layers of coating, and number of runs (stability). The coated capillaries showed a highly stable mu(EOF) (run-to-run RSD less than 1.5%, n = 20), allowing continuous use for several days without conditioning. The coated capillaries were then used for the effective separation of nine environmentally important phenolic compounds showing a significant improvement in the resolution, when compared to bare fused-silica capillaries. The EOF of the coated capillaries was constant in alkaline solutions (pH > or = 7), allowing the optimization of the separation conditions of phenolic compounds without significantly affecting the mu(EOF).

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“…SMZ and TMP were simultaneously determined through CZE with UV detection [15], [16] and [17] in fused silica capillaries. A polyamine coated capillary was used to separate TMP and some of its decomposition products including 3,4,5-trimethoxybenzoic acid (TBA) [18]. A microfluidic capillary electrophoresis method combined with flow injection analysis was developed for the on-line concentration and separation of SMZ and TMP [19].…”

Section: Introductionmentioning

confidence: 99%

Assessment of MEKC suitability for residue drug monitoring on pharmaceutical manufacturing equipment

Boca¹,

Pretorius²,

Kgaje³

et al. 2008

Journal of Pharmaceutical and Biomedical Analysis

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The suitability of micellar electrokinetic chromatography for the simultaneous trace determination of several compounds (sulfamethoxazole, trimethoprim, sulfanilic acid, sulfanilamide, 3,4,5-trimethoxybenzoic acid and nonoxynol-9) was assessed. The mixture was separated within 14 min at an applied voltage of 22 kV by using 30 mM phosphate electrolyte, containing 10 mM SDS, adjusted to pH 7.8. Under optimized separation conditions acceptable levels of linearity, precision and accuracy were obtained for all compounds. The method could be used as part of a cleaning validation study when assaying trace levels of co-trimoxazole drug, some of its decomposition products and detergent in the swab samples collected from pharmaceutical manufacturing equipment, after cleaning.

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Rapid resolution of drugs and related substances with an eCAPTM polyamine coated capillary

Cited by 20 publications

References 7 publications

Impurity analysis of pharmaceuticals using capillary electromigration methods

Impurity analysis of pharmaceuticals using capillary electromigration methods

Electrophoretic separation of environmentally important phenolic compounds using montomorillonite‐coated fused‐silica capillaries

Assessment of MEKC suitability for residue drug monitoring on pharmaceutical manufacturing equipment

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