Rapid recognition and functional analysis of membrane proteins on human cancer cells using atomic force microscopy

Li, Mi; Xiao, Xiubin; Liu, Lianqing; Xi, Ning; Wang, Yuechao

doi:10.1016/j.jim.2016.06.006

Cited by 10 publications

(8 citation statements)

References 39 publications

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“…To obtain a force curve, the AFM probe was controlled to perform an approach-retract cycle in the vertical direction on the substrate. 30 Researchers have widely used AFM force spectroscopy to investigate the adhesion forces of adhesives, such as DOPA polymer adhesive, 12 adhesive mimicking gecko foot-hair, 31 carbohydrate hydrogel, 32 and alginate hydrogel. 33 Here, we used the AFM to characterize the adhesion characteristics of the fabricated Sundew-inspired hydrogel.…”

Section: Resultsmentioning

confidence: 99%

“…Figure shows adhesion characteristics of the fabricated Sundew-inspired hydrogels by AFM. To obtain a force curve, the AFM probe was controlled to perform an approach–retract cycle in the vertical direction on the substrate . Researchers have widely used AFM force spectroscopy to investigate the adhesion forces of adhesives, such as DOPA polymer adhesive, adhesive mimicking gecko foot-hair, carbohydrate hydrogel, and alginate hydrogel .…”

Section: Resultsmentioning

confidence: 99%

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A Bioinspired Alginate-Gum Arabic Hydrogel with Micro-/Nanoscale Structures for Controlled Drug Release in Chronic Wound Healing

Li²,

et al. 2017

ACS Appl. Mater. Interfaces

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132

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Biopolymeric hydrogels have drawn increasing research interest in biomaterials due to their tunable physical and chemical properties for both creating bioactive cellular microenvironment and serving as sustainable therapeutic reagents. Inspired by a naturally occurring hydrogel secreted from the carnivorous Sundew plant for trapping insects, here we have developed a bioinspired hydrogel to deliver mitsugumin 53 (MG53), an important protein in cell membrane repair, for chronic wound healing. Both chemical compositions and micro-/nanomorphological properties inherent from the natural Sundew hydrogel were mimicked using sodium alginate and gum arabic with calcium ion-mediated cross-linking. On the basis of atomic force microscopy (AFM) force measurements, an optimal sticky hydrogel scaffold was obtained through orthogonal experimental design. Imaging and mechanical analysis showed the distinct correlation between structural morphology, adhesion characteristics, and mechanical properties of the Sundew-inspired hydrogel. Combined characterization and biochemistry techniques were utilized to uncover the underlying molecular composition involved in the interactions between hydrogel and protein. In vitro drug release experiments confirmed that the Sundew-inspired hydrogel had a biphasic-kinetics release, which can facilitate both fast delivery of MG53 for improving the reepithelization process of the wounds and sustained release of the protein for treating chronic wounds. In vivo experiments showed that the Sundew-inspired hydrogel encapsulating with rhMG53 could facilitate dermal wound healing in mouse model. Together, these studies confirmed that the Sundew-inspired hydrogel has both tunable micro-/nanostructures and physicochemical properties, which enable it as a delivery vehicle for chronic wounding healing. The research may provide a new way to develop biocompatible and tunable biomaterials for sustainable drug release to meet the needs of biological activities.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

A Bioinspired Alginate-Gum Arabic Hydrogel with Micro-/Nanoscale Structures for Controlled Drug Release in Chronic Wound Healing

Li²,

et al. 2017

ACS Appl. Mater. Interfaces

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132

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“…Recently, a new AFM imaging mode, which is called PFT [ 26 , 27 ], became commercially available for multiparametric imaging, as shown in Figure 1 B. In the PFT mode, the vibrating tip indents the samples to record an array of force curves for each sampling points on the specimens.…”

Section: Afm Single-molecule Techniquesmentioning

confidence: 99%

“…First, force curves are recorded on a stiff substrate (such as glass) to obtain the deflection sensitivity of the cantilever (nm/v). Then the spring constant of the cantilever is calibrated by thermal noise method [ 27 ]. The force resolution of AFM is determined by its spring constant.…”

Section: Afm Single-molecule Techniquesmentioning

confidence: 99%

Imaging and Force Recognition of Single Molecular Behaviors Using Atomic Force Microscopy

Dang

Liu

et al. 2017

Sensors

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The advent of atomic force microscopy (AFM) has provided a powerful tool for investigating the behaviors of single native biological molecules under physiological conditions. AFM can not only image the conformational changes of single biological molecules at work with sub-nanometer resolution, but also sense the specific interactions of individual molecular pair with piconewton force sensitivity. In the past decade, the performance of AFM has been greatly improved, which makes it widely used in biology to address diverse biomedical issues. Characterizing the behaviors of single molecules by AFM provides considerable novel insights into the underlying mechanisms guiding life activities, contributing much to cell and molecular biology. In this article, we review the recent developments of AFM studies in single-molecule assay. The related techniques involved in AFM single-molecule assay were firstly presented, and then the progress in several aspects (including molecular imaging, molecular mechanics, molecular recognition, and molecular activities on cell surface) was summarized. The challenges and future directions were also discussed.

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“…Especially the development of high-speed AFM brings new opportunities for studying the structure and dynamics of single biomolecules in action [12]. In the past decade, the applications of AFM in DNA-related research have provided plenty of novel insights into the behaviors of single DNAs [13][14][15][16][17][18]. Researchers have applied AFM to investigate the interactions between diverse drugs and DNA molecules [19][20][21][22][23][24], but these studies are commonly done with linear DNAs.…”

Section: Introductionmentioning

confidence: 99%

The dynamic interactions between chemotherapy drugs and plasmid DNA investigated by atomic force microscopy

Liu

Xiao

et al. 2017

Sci. China Mater.

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The advent of atomic force microscopy (AFM) provides a powerful tool for imaging individual DNA molecules. Chemotherapy drugs are often related to DNAs. Though many specific drug-DNA interactions have been observed by AFM, knowledge about the dynamic interactions between chemotherapy drugs and plasmid DNAs is still scarce. In this work, AFM was applied to investigate the nanoscale interactions between plasmid DNAs and two commercial chemotherapy drugs (methotrexate and cisplatin). Plasmid DNAs were immobilized on mica which was coated by silanes in advance. AFM imaging distinctly revealed the dynamic changes of single plasmid DNAs after the stimulation of methotrexate and cisplatin. Geometric features of plasmid DNAs were extracted from AFM images and the statistical results showed that the geometric features of plasmid DNAs changed significantly after the stimulation of drugs. This research provides a novel idea to study the actions of chemotherapy drugs against plasmid DNAs at the single-molecule level.

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Rapid recognition and functional analysis of membrane proteins on human cancer cells using atomic force microscopy

Cited by 10 publications

References 39 publications

A Bioinspired Alginate-Gum Arabic Hydrogel with Micro-/Nanoscale Structures for Controlled Drug Release in Chronic Wound Healing

A Bioinspired Alginate-Gum Arabic Hydrogel with Micro-/Nanoscale Structures for Controlled Drug Release in Chronic Wound Healing

Imaging and Force Recognition of Single Molecular Behaviors Using Atomic Force Microscopy

The dynamic interactions between chemotherapy drugs and plasmid DNA investigated by atomic force microscopy

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