Rapid real-time recirculating PCR using localized surface plasmon resonance (LSPR) and piezo-electric pumping

Haber, J.; Gascoyne, Peter R. C.; Sokolov, Konstantin

doi:10.1039/c7lc00211d

Cited by 27 publications

(13 citation statements)

References 49 publications

(50 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Haber et al [63] recently integrated on-chip DNA hybridization and real-time PCR using an LSPR-based sensor. They outlined the development of a novel microfluidic sensor for the implementation of qPCR, employing a piezo-electric pumping microsystem.…”

Section: Higher Integration Platformsmentioning

confidence: 99%

See 1 more Smart Citation

Microfluidic Devices for Label-Free DNA Detection

et al. 2018

View full text Add to dashboard Cite

Sensitive and specific DNA biomarker detection is critical for accurately diagnosing a broad range of clinical conditions. However, the incorporation of such biosensing structures in integrated microfluidic devices is often complicated by the need for an additional labelling step to be implemented on the device. In this review we focused on presenting recent advances in label-free DNA biosensor technology, with a particular focus on microfluidic integrated devices. The key biosensing approaches miniaturized in flow-cell structures were presented, followed by more sophisticated microfluidic devices and higher integration examples in the literature. The option of full DNA sequencing on microfluidic chips via nanopore technology was highlighted, along with current developments in the commercialization of microfluidic, label-free DNA detection devices.

show abstract

Section: Higher Integration Platformsmentioning

confidence: 99%

“…Structural microfluidic channel layout of Polymerase Chain Reaction (PCR) chip (Reprinted from Haberet al[63]. Copyright (2017), with permission from Royal Society of Chemistry).…”

mentioning

confidence: 99%

Microfluidic Devices for Label-Free DNA Detection

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Polypropylene (PP) and polycarbonate (PC) are commonly used to design the tubes for the reaction chambers, which require a bulky and complicated design of the thermal cycler to achieve temperature conditions with good thermal uniformity. In addition, PC [15], cyclic olefin polymer (COP) [16], cyclic olefin copolymer (COC) [17], polymethyl methacrylate (PMMA) [18], polyethylene terephthalate (PET) [19], and polydimethylsiloxane (PDMS) [20] are frequently used to fabricate PCR chips; however, these most commonly used materials have many limitations, such as poor thermal conductivity, water permeability, and air permeability. The common methods for detecting the PCR product are agarose electrophoresis detection and fluorescence microscopy detection, both of which inevitably require a high power supply and complicated operation.…”

Section: Introductionmentioning

confidence: 99%

Portable and Battery-Powered PCR Device for DNA Amplification and Fluorescence Detection

Jie

Liu

et al. 2020

Sensors

View full text Add to dashboard Cite

Polymerase chain reaction (PCR) is a technique for nucleic acid amplification, which has been widely used in molecular biology. Owing to the limitations such as large size, high power consumption, and complicated operation, PCR is only used in hospitals or research institutions. To meet the requirements of portable applications, we developed a fast, battery-powered, portable device for PCR amplification and end-point detection. The device consisted of a PCR thermal control system, PCR reaction chip, and fluorescence detection system. The PCR thermal control system was formed by a thermal control chip and external drive circuits. Thin-film heaters and resistance temperature detectors (RTDs) were fabricated on the thermal control chip and were regulated with external drive circuits. The average heating rate was 32 °C/s and the average cooling rate was 7.5 °C/s. The disposable reaction chips were fabricated using a silicon substrate, silicone rubber, and quartz plate. The fluorescence detection system consisted a complementary metal-oxide-semiconductor (CMOS) camera, an LED, and mirror units. The device was driven by a 24 V Li-ion battery. We amplified HPV16E6 genomic DNA using our device and achieved satisfactory results.

show abstract

“…Touahir et al (2010) proposed a microfluidic DNA sensing approach based on metal nanostructure enhanced fluor escence, but this requires fluorescence labeling of the DNA probes. More recently, Haber et al were able to monitor DNA hybridization in real time by combining sensor chips with silver nanoprism structures with a microfluidic setup in a label free manner (Haber et al, 2017). However, to our knowledge, no work on LSPR detection of DNA poly merase reaction in real time has been reported in literature.…”

Section: Introductionmentioning

confidence: 99%

Real-time monitoring of DNA immobilization and detection of DNA polymerase activity by a microfluidic nanoplasmonic platform

Roether

Chu

Willenbacher

et al. 2019

Biosensors and Bioelectronics

View full text Add to dashboard Cite

DNA polymerase catalyzes the replication of DNA, one of the key steps in cell division. The control and understanding of this reaction owns great potential for the fundamental study of DNA-enzyme interactions. In this context, we developed a label-free microfluidic biosensor platform based on the principle of localized surface plasmon resonance (LSPR) to detect the DNA-polymerase reaction in real-time. Our microfluidic LSPR chip integrates a polydimethylsiloxane (PDMS) channel bonded with a nanoplasmonic substrate, which consists of densely packed mushroom-like nanostructures with silicon dioxide stems (~40 nm) and gold caps (~22 nm), with an average spacing of 19 nm. The LSPR chip was functionalized with single-stranded DNA (ssDNA) template (T30), spaced with hexanedithiol (HDT) in a molar ratio of 1:1. The DNA primer (P8) was then attached to T30, and the second strand was subsequently elongated by DNA polymerase assembling nucleotides from the surrounding fluid. All reaction steps were detected in-situ inside the microfluidic LSPR chip, at room temperature, in real-time, and label-free. In addition, the sensor response was successfully correlated with the amount of DNA and HDT molecules immobilized on the LSPR sensor surface. Our platform represents a benchmark in developing microfluidic LSPR chips for DNA-enzyme interactions, further driving innovations in biosensing technologies.

show abstract

Rapid real-time recirculating PCR using localized surface plasmon resonance (LSPR) and piezo-electric pumping

Cited by 27 publications

References 49 publications

Microfluidic Devices for Label-Free DNA Detection

Microfluidic Devices for Label-Free DNA Detection

Portable and Battery-Powered PCR Device for DNA Amplification and Fluorescence Detection

Real-time monitoring of DNA immobilization and detection of DNA polymerase activity by a microfluidic nanoplasmonic platform

Contact Info

Product

Resources

About