Microfluidic Devices for Label-Free DNA Detection

Dutta, Gorachand; Rainbow, Joshua; Zupančič, Uroš; Papamatthaiou, Sotirios; Estrela, Pedro; Moschou, Despina

doi:10.3390/chemosensors6040043

Cited by 41 publications

(20 citation statements)

References 89 publications

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“…The extraction of target analyte from the pathogenic microbe of interest is a four-step process: (1) cell lysis; (2) separation of target proteins, nucleic acids, etc. ; (3) target purification; and (4) target preconcentration [126,127]. Steps 2 and 3 can be done using the methods of filtration and mixing discussed previously with integration of microfluidic channels and chambers.…”

Section: How To Detect On-sitementioning

confidence: 99%

Integrated Electrochemical Biosensors for Detection of Waterborne Pathogens in Low-Resource Settings

et al. 2020

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More than 783 million people worldwide are currently without access to clean and safe water. Approximately 1 in 5 cases of mortality due to waterborne diseases involve children, and over 1.5 million cases of waterborne disease occur every year. In the developing world, this makes waterborne diseases the second highest cause of mortality. Such cases of waterborne disease are thought to be caused by poor sanitation, water infrastructure, public knowledge, and lack of suitable water monitoring systems. Conventional laboratory-based techniques are inadequate for effective on-site water quality monitoring purposes. This is due to their need for excessive equipment, operational complexity, lack of affordability, and long sample collection to data analysis times. In this review, we discuss the conventional techniques used in modern-day water quality testing. We discuss the future challenges of water quality testing in the developing world and how conventional techniques fall short of these challenges. Finally, we discuss the development of electrochemical biosensors and current research on the integration of these devices with microfluidic components to develop truly integrated, portable, simple to use and cost-effective devices for use by local environmental agencies, NGOs, and local communities in low-resource settings.

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Section: How To Detect On-sitementioning

confidence: 99%

Integrated Electrochemical Biosensors for Detection of Waterborne Pathogens in Low-Resource Settings

et al. 2020

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“…In POC testing, various detection techniques such as nucleic acid testing ( Batule et al, 2020 ; Leiske et al, 2015 ), lateral flow assays (LFA) ( Fang et al, 2014 ; R. H. Tang et al, 2017 ), nanomaterial-based sensors ( Li et al, 2020 ; Liu et al, 2014 ; Ngo et al, 2018 ; Padmavathy et al, 2012 ), colorimetric immunosensors ( Ren et al, 2017 ), volatile organic compound sensors (Z. Li et al, 2019a , Li et al, 2019b ), bio-optical sensors ( Jin et al, 2018 ; Yoo and Lee, 2016 ), and electrochemical sensors ( Dutta et al, 2018 ; W. Liu et al, 2018 ) have been applied for the rapid detection of a broad range of human and plant diseases. Among these techniques, molecular assays based on nucleic acid amplification (NAA) are widely preferred.…”

Section: Introductionmentioning

confidence: 99%

Advances in point-of-care nucleic acid extraction technologies for rapid diagnosis of human and plant diseases

Paul

Ostermann

Wei

2020

Biosensors and Bioelectronics

118

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Global health and food security constantly face the challenge of emerging human and plant diseases caused by bacteria, viruses, fungi, and other pathogens. Disease outbreaks such as SARS, MERS, Swine Flu, Ebola, and COVID-19 (on-going) have caused suffering, death, and economic losses worldwide. To prevent the spread of disease and protect human populations, rapid point-of-care (POC) molecular diagnosis of human and plant diseases play an increasingly crucial role. Nucleic acid-based molecular diagnosis reveals valuable information at the genomic level about the identity of the disease-causing pathogens and their pathogenesis, which help researchers, healthcare professionals, and patients to detect the presence of pathogens, track the spread of disease, and guide treatment more efficiently. A typical nucleic acid-based diagnostic test consists of three major steps: nucleic acid extraction, amplification, and amplicon detection. Among these steps, nucleic acid extraction is the first step of sample preparation, which remains one of the main challenges when converting laboratory molecular assays into POC tests. Sample preparation from human and plant specimens is a time-consuming and multi-step process, which requires well-equipped laboratories and skilled lab personnel. To perform rapid molecular diagnosis in resource-limited settings, simpler and instrument-free nucleic acid extraction techniques are required to improve the speed of field detection with minimal human intervention. This review summarizes the recent advances in POC nucleic acid extraction technologies. In particular, this review focuses on novel devices or methods that have demonstrated applicability and robustness for the isolation of high-quality nucleic acid from complex raw samples, such as human blood, saliva, sputum, nasal swabs, urine, and plant tissues. The integration of these rapid nucleic acid preparation methods with miniaturized assay and sensor technologies would pave the road for the “sample-in-result-out” diagnosis of human and plant diseases, especially in remote or resource-limited settings.

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“…Some particular application examples include wastewater treatment [7], desalination [8,9], removal of toxic chemicals [10], of bacterial and viral agents [11] and gathering and concentration of targeted species (e.g., precious metals) from seawater [12]. Another wide field of use is biomedicine and life sciences [13], and covers such diverse areas as biointerfaces including brain-machine interfaces [14], scaffolds for tissue growth [15,16], biosensors [17,18], drug delivery and targeting [19], various labs-on-a-chip [20], DNA sequencers [21], etc. A field where the benefits of biomimetic nanomembranes are already starting to lead to large advancements is renewable energy [22].…”

Section: Introductionmentioning

confidence: 99%

Biomimetic Nanomembranes: An Overview

Jakšić

2020

Biomimetics

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Nanomembranes are the principal building block of basically all living organisms, and without them life as we know it would not be possible. Yet in spite of their ubiquity, for a long time their artificial counterparts have mostly been overlooked in mainstream microsystem and nanosystem technologies, being a niche topic at best, instead of holding their rightful position as one of the basic structures in such systems. Synthetic biomimetic nanomembranes are essential in a vast number of seemingly disparate fields, including separation science and technology, sensing technology, environmental protection, renewable energy, process industry, life sciences and biomedicine. In this study, we review the possibilities for the synthesis of inorganic, organic and hybrid nanomembranes mimicking and in some way surpassing living structures, consider their main properties of interest, give a short overview of possible pathways for their enhancement through multifunctionalization, and summarize some of their numerous applications reported to date, with a focus on recent findings. It is our aim to stress the role of functionalized synthetic biomimetic nanomembranes within the context of modern nanoscience and nanotechnologies. We hope to highlight the importance of the topic, as well as to stress its great applicability potentials in many facets of human life.

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Microfluidic Devices for Label-Free DNA Detection

Cited by 41 publications

References 89 publications

Integrated Electrochemical Biosensors for Detection of Waterborne Pathogens in Low-Resource Settings

Integrated Electrochemical Biosensors for Detection of Waterborne Pathogens in Low-Resource Settings

Advances in point-of-care nucleic acid extraction technologies for rapid diagnosis of human and plant diseases

Biomimetic Nanomembranes: An Overview

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