Rapid, quantitative high-performance liquid column chromatography of pseudouridine

Kuo, Kenneth C.; Gehrke, Charles W.; McCune, Roy A.; Waalkes, T. Phillip; Borek, Ernest

doi:10.1016/s0378-4347(00)81367-5

Cited by 39 publications

(4 citation statements)

References 9 publications

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“…Several column chromatographic methods for the analysis of pyrimidines and purines in the urine have been described, including conventional cationexchange chromatography [ 29,301, HPLC anion-exchange chromatography and HPLC using normal-phase [36] and reverse-phase [37,38] columns. The conventional cation-exchange methods have the capability of separating numerous pyrimidine and purine bases and nucleosides in urine, but on the other hand have the disadvantages of long run times and regeneration steps, and the use of acid buffers, causing decomposition of purine nucleosides and deoxynucleosides.…”

Section: Discussionmentioning

confidence: 99%

Urinary purines and pyrimidines in patients with hyperammonemia of various origins

Gennip

Bree-Blom

Grift

et al. 1980

Clinica Chimica Acta

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SummaryExcretion patterns of pyrimidines and purines in patients with various types of hyperammonemia have been investigated by a-dimensional thin-layer chromatography and high pressure liquid chromatography (HPLC). For the quantitative analysis of pseudouridine, uracil and uridine a new procedure has been developed, consisting of pre-fractionation with Dowex 1 X 8, followed by dual column HPLC on a strong anion-exchanger and a reverse phase column. Thymine has also been analyzed in the pre-fractionated urine by a new HPLC method using the reverse phase column in combination with a strong cationexchange column. Quantitative data for urinary pyrimidines and uric acid in hyperammonemia are given. In patients with a defect in one of the urea cycle enzymes, the level of pyrimidine excretion was found to depend on plasma ammonia concentrations.In other hyperammonemic patients, an increased excretion of erotic acid, uracil and uridine has only been found in one of the two pati'ents with lysinuric protein intolerance, all other patients showing normal excretion patterns, Elevated uric acid excretions have been found frequently in our patients with hyper~monemia, but they did not always coincide with high plasma ammonia levels. A possible explanation for the difference in the excretion levels of the various pyrimidines is discussed.

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Section: Discussionmentioning

confidence: 99%

Urinary purines and pyrimidines in patients with hyperammonemia of various origins

Gennip

Bree-Blom

Grift

et al. 1980

Clinica Chimica Acta

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“…The Y concentration was determined by reversed-phase high performance liquid chromatography (19)(20)(21)(22)(23), specifically developed for quantitative measurements of tRNAmodified nucleosides in biological samples. Initial partial purification of ribonucleosides using an affinity gel containing an immobilized phenyl boronic acid was performed in order to improve selectivity and sensitivity.…”

Section: Methodsmentioning

confidence: 99%

Urinary Excretion of Pseudouridine in Patients with Brain Tumours

Shantaram

Aroor

Raja³

et al. 1993

Acta Oncologica

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“…Da der Nukleinsäure-Turnover proliferierender Zellen verändert ist, kann dieser durch Pseudouridin erfaßt werden (I, 5,6,7,36). Eine Reihe Untersuchungen verschiedener Laboratorien (1,5-10, 14, 16, 21-23, 25…”

Section: The Ciinical Value Of Urinary Excretion Of Pseudouridine In unclassified

“…Unsere Daten zeigen, wie die anderer Untersucher bei Erwachsenen auch, daß die Bestimmung der Pseudouridinausscheidung bei den verschiedensten Erkrankungen erhöht sein kann (5-12, 14, 16,21-23,25-29, 31,32,34-37,39,40,42-45). Diese erhöhten Werte werden nicht nur bei malignen Erkrankungen gefunden, wenngleich sie dort die wichtigste Rolle spielen und hier zum Teil als biochemischer Tumormarker angesehen werden (8,10,21,22,25,26,29,32,34,(35)(36)(37)39,42,43 schen Darstellung folgt eine Diskussion der zugrundeliegenden Wertfragen unter Überschriften wie "Fundamentalkritik", "Wertsetzung", "Rechtsbedenken", "Rassismus", "Neo-Eugenik? ".…”

Section: Diskussionunclassified

Die klinische Bedeutung der Pseudouridinbestimmung im Urin von Kindern und Jugendlichen*

Graf

Bach²,

Frisch³

et al. 1989

Klin Padiatr

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A HPLC-method is described to determine the Pseudouridine/creatinine ratio in spontaneous urine samples in infancy and childhood. The urines of 74 healthy children between 1 and 18 years of age and of 231 children with different diseases were examined for this ratio, making 1097 measurements. 157 children suffered from a malignant disease, 66 of them having an acute leukemia. Those patients, who remain in remission of the leukemia showed normal values, whereas the others had elevated ratios, reflecting the activity of the leukemia, when they were followed up by multiple determinations. Perhaps it is also possible to detect preclinical stages of leukemia by measuring the pseudouridine/creatinine ratio routinously over a long period of time. Today no strict correlation between the prognosis of leukemia and the level of this ratio can be drawn. Similar behaviour of the pseudouridine/creatinine ratio is seen in other malignant diseases with exception of brain tumors. The difference to leukemias is, that all other malignant tumors show more often normal values in patients with a remaining tumor. A pathological value of pseudouridine may also be seen in others than malignant diseases.

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Rapid, quantitative high-performance liquid column chromatography of pseudouridine

Cited by 39 publications

References 9 publications

Urinary purines and pyrimidines in patients with hyperammonemia of various origins

Urinary purines and pyrimidines in patients with hyperammonemia of various origins

Urinary Excretion of Pseudouridine in Patients with Brain Tumours

Die klinische Bedeutung der Pseudouridinbestimmung im Urin von Kindern und Jugendlichen*

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