Die klinische Bedeutung der Pseudouridinbestimmung im Urin von Kindern und Jugendlichen*

Abstract

A HPLC-method is described to determine the Pseudouridine/creatinine ratio in spontaneous urine samples in infancy and childhood. The urines of 74 healthy children between 1 and 18 years of age and of 231 children with different diseases were examined for this ratio, making 1097 measurements. 157 children suffered from a malignant disease, 66 of them having an acute leukemia. Those patients, who remain in remission of the leukemia showed normal values, whereas the others had elevated ratios, reflecting the activity of the leukemia, when they were followed up by multiple determinations. Perhaps it is also possible to detect preclinical stages of leukemia by measuring the pseudouridine/creatinine ratio routinously over a long period of time. Today no strict correlation between the prognosis of leukemia and the level of this ratio can be drawn. Similar behaviour of the pseudouridine/creatinine ratio is seen in other malignant diseases with exception of brain tumors. The difference to leukemias is, that all other malignant tumors show more often normal values in patients with a remaining tumor. A pathological value of pseudouridine may also be seen in others than malignant diseases.

Comparison of Urinary Neopterin and Pseudouridine in Patients with Malignant Proliferative Diseases

“…Determination of neopterin in blood plasma with a peak at 06.30 h (4). There is a considerable number of publications concerning changes in urinary pseudouridine excretion evoked by malignant growth (9)(10)(11)(12)(13)(14)(15)). Neopterin is urinary and/or serum neopterin concentrations: haesynthesized from guanosine triphosphate by macro-matological neoplasms, gynaecological tumours, tumphages by the action of GTP cyclohydrolase, an en-ours of the genitourinary tract, lung cancer, gastrozyme induced by interferon gamma and derived from intestinal and pancratic carcinoma, melanomas, _ breast tumours, head and neck cancers.…”

Age-dependence of urinary normal and modified nucleosides in childhood as determined by reversed-phase high-performance liquid chromatography

“…However, they did not include children younger than 4 years. Graf et al [28] described for a group of 74 healthy children between 1 and 18 years of age a decrease of the levels of Pseu using curve fitting with a polynomial of fourth degree. In all these studies, relatively small numbers of children and only few of the nucleosides were included.…”