Rapid quantification of malondialdehyde in plasma by high performance liquid chromatography–visible detection

Grotto, Denise; Maria, Letizia De; Boeira, Silvana Peterini; Valentini, Juliana; Charão, Mariele Feiffer; Moro, Ângela M.; Nascimento, Paulo Cı́cero do; Pomblum, Valdeci Juarez; Garcia, Solange Cristina

doi:10.1016/j.jpba.2006.07.030

Cited by 294 publications

(196 citation statements)

References 19 publications

Supporting

Mentioning

174

Contrasting

Unclassified

Order By: Relevance

“…Peripheral insulin resistance was assessed via the homeostasis model assessment (HOMA) as described in the previous studies (Haffner et al 1997, Appleton et al 2005. Plasma malondialdehyde (MDA) was determined using a HPLC-based assay (Grotto et al 2007).…”

Section: Plasma Analysismentioning

confidence: 99%

DPP-4 inhibitors improve cognition and brain mitochondrial function of insulin-resistant rats

Pintana

Apaijai²,

Chattipakorn³

2013

Journal of Endocrinology

169

View full text Add to dashboard Cite

Recent evidence has demonstrated that insulin resistance is related to the development of type 2 diabetes mellitus. Our previous study found that high-fat diet (HFD) consumption caused not only peripheral and brain insulin resistance but also brain mitochondrial dysfunction and cognitive impairment. Vildagliptin and sitagliptin, dipeptidyl-peptidase-4 inhibitors, are recently developed anti-diabetic drugs. However, the effects of both drugs on cognitive behaviors and brain mitochondrial function in HFD-induced insulin-resistant rats have not yet been investigated. Sixty male Wistar rats were divided into two groups to receive either normal diet or HFD for 12 weeks. Rats in each group were then further divided into three treatment groups to receive either vehicle, vildagliptin (3 mg/kg per day), or sitagliptin (30 mg/kg per day) for 21 days. The cognitive behaviors of the rats were tested using the Morris Water Maze test. Blood samples were collected to determine metabolic parameters and plasma oxidative stress levels. Upon completion of the study, the animals were killed and the brains were removed to investigate brain and hippocampal mitochondrial function as well as to determine oxidative stress levels. We demonstrated that both drugs significantly improved the metabolic parameters and decreased circulating and brain oxidative stress levels in HFD-induced insulin-resistant rats. In addition, both drugs completely prevented brain and hippocampal mitochondrial dysfunction and equally improved the learning behaviors impaired by the HFD. Our findings suggest that the inhibition of dipeptidyl-peptidase-4 enzymes with vildagliptin or sitagliptin in insulinresistant rats not only increases peripheral insulin sensitivity but also decreases brain dysfunction. Key Words" insulin resistance " high-fat diet " DDP-4 inhibitors " memory decline " brain mitochondrial dysfunction

show abstract

Section: Plasma Analysismentioning

confidence: 99%

DPP-4 inhibitors improve cognition and brain mitochondrial function of insulin-resistant rats

Pintana

Apaijai²,

Chattipakorn³

2013

Journal of Endocrinology

169

View full text Add to dashboard Cite

show abstract

“…Concentration of glutathione (GSH) was determined using the method of Buetler et al (1963) and was expressed in mmol/l. Concentration of total MDA in plasma was determined by HPLC (Grotto et al, 2007) on TSP-130 system (Thermo Separation Products Inc., Thermo Fisher Scientific Inc., Waltham, MA, USA).…”

Section: Experimental Design and Dietsmentioning

confidence: 99%

Immune and oxidative response to linseed in the diet of periparturient Holstein cows

Đidara

Poljičak-Milas

Milinković-Tur³

et al. 2015

Animal

View full text Add to dashboard Cite

The aim of this research was to determine the influence of dietary replacement of n-6 with n-3 polyunsaturated fatty acids on cellular immunity and oxidative stress in the transition period dairy cows. The experiment was conducted on 20 dairy Holstein cows from 3 ± 1 weeks before parturition until the 6 th week of lactation. Both groups were fed an iso-energetic and iso-nitrogenous diet. Soybean meal from control (C) group was replaced with linseed in the experimental (LS) group. Cellular immunity and oxidative stress were measured on days −10, 1, 21 and 42 relative to parturition. During the entire experimental period, the proportion of CD45 + cells was lower ( P < 0.05) in LS group compared with the C group. The phagocytosis ability and phagocytosis index of cows fed with n-3 fatty acids were significantly reduced ( P < 0.05) compared with the group of cows fed with n-6 fatty acids. The most severe decrease in phagocytosis ability was on day −10 and 1 relative to parturition. The activity of superoxide dismutase ( P < 0.05) and plasma glutathione peroxidase ( P < 0.05) increased around calving, although activities were not influenced by dietary treatment. Increased malondialdehyde concentration ( P < 0.05) was influenced by dietary n-3 fatty acids and the time relative to parturition. The immune suppression was most pronounced during periparturient period. In that matter we can conclude that not only dietary n-3 fatty acids but also oxidative stress, which reached peak at time of parturition, contributed to the reduced cellular immunity during the periparturient period.

show abstract

“…Na derivatização do MDA com TBA (Londero, Greco,1996;Grotto et al, 2007), as condições experimentais propiciam a geração in vitro de produtos de lipoperoxidação, contribuindo para uma menor especificidade do método. O uso de DNPH como derivatizante apresenta como vantagem, além da melhoria das características de absortividade no UV, o uso de condições reacionais mais brandas, em temperatura ambiente.…”

Section: Derivatizaçãounclassified

“…Com o propósito de obter determinações mais específicas e sensíveis para a quantificação do MDA em amostras biológicas, diversas metodologias com emprego de cromatografia líquida de alta eficiência (CLAE) têm sido propostas (Steghens et al, 2001;Pilz, Meineke, Gleiter, 2000;Sim et al, 2003;Karatas, Karatepe, Baysar, 2002;Londero, Greco, 1996;Mao et al, 2006;Grotto et al, 2007). Os métodos propostos por Steghens et al (2001), Pilz, Meineke, Gleiter (2000) e Mao et al (2006) quantificam, além do MDA total, também a sua forma livre.…”

Section: Introductionunclassified

Estudo pré-analítico e de validação para determinação de malondialdeído em plasma humano por cromatografia líquida de alta eficiência, após derivatização com 2,4-dinitrofenilhidrazina

Antunes¹,

Lazzaretti²,

Gamaro³

et al. 2008

Rev. Bras. Cienc. Farm.

View full text Add to dashboard Cite

Rapid quantification of malondialdehyde in plasma by high performance liquid chromatography–visible detection

Cited by 294 publications

References 19 publications

DPP-4 inhibitors improve cognition and brain mitochondrial function of insulin-resistant rats

DPP-4 inhibitors improve cognition and brain mitochondrial function of insulin-resistant rats

Immune and oxidative response to linseed in the diet of periparturient Holstein cows

Estudo pré-analítico e de validação para determinação de malondialdeído em plasma humano por cromatografia líquida de alta eficiência, após derivatização com 2,4-dinitrofenilhidrazina

Contact Info

Product

Resources

About