Estudo pré-analítico e de validação para determinação de malondialdeído em plasma humano por cromatografia líquida de alta eficiência, após derivatização com 2,4-dinitrofenilhidrazina

Antunes, Marina Venzon; Lazzaretti, Camilla; Gamaro, Giovana Duzzo; Linden, Rafael

doi:10.1590/s1516-93322008000200013

Cited by 30 publications

(18 citation statements)

References 20 publications

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“…The products of lipid peroxidation of phospholipids present in cell membranes, such as MDA, can be used as indicators of OFR action in the body. 39 By far, MDA determination is the most popular indicator of oxidative damage to cells and tissues. 39 We noted that in the first two weeks of intervention with SCF, a significant reduction in MDA tissue content already had taken place, even when we applied a lower concentration of the substance.…”

Section: Discussionmentioning

confidence: 99%

“…39 By far, MDA determination is the most popular indicator of oxidative damage to cells and tissues. 39 We noted that in the first two weeks of intervention with SCF, a significant reduction in MDA tissue content already had taken place, even when we applied a lower concentration of the substance. MDA tissue content remained low after four weeks of intervention, regardless of SCF concentration used.…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of the anti-inflammatory and antioxidant effects of the sucralfate in diversion colitis

Rodrigues

Sato

Silva

et al. 2015

Journal of Coloproctology

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Evaluation of the anti-inflammatory and antioxidant effects of the sucralfate in diversion colitis

Rodrigues

Sato

Silva

et al. 2015

Journal of Coloproctology

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“…According to the method described by Antunes et al [24], MDA dosages were initiated with alkaline hydrolysis of plasma in order to release the proteinbound fraction, subsequently precipitated by the addition of HClO 4 . DNPH-derivatized protein was added to the supernatant.…”

Section: Monthmentioning

confidence: 99%

Oxidative stress in patients with type 2 diabetes mellitus treated with metformin

Dries¹,

Soares

Ziulkoski

et al. 2017

Sci Med

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AIMS: To evaluate oxidative stress parameters in patients with type 2 diabetes mellitus treated with metformin, relating these values to its side effects, plasma levels, glycemic control, diabetic complications, lipid profile, and the influence of pharmacotherapeutic follow-up. METHODS: Patients with type 2 diabetes mellitus, on metformin and in pharmacotherapeutic follow-up for four months, were evaluated. The pharmacotherapeutic follow-up consisted in providing information and answering patients' questions about medication and disease. In addition, administration times, dosages, and presence or absence of side effects related to the use of metformin were verified. Glycemic and lipid profile, oxidative stress (superoxide dismutase and malondialdehyde) and plasma metformin were evaluated. Pearson's correlation and Spearman's correlation were performed to evaluate the relationship between the variables at the beginning of the study. The independent t-test and Mann-Whitney U test were used to assess the difference between the groups with and without diabetic complications. The range of values between the beginning and end of the study was evaluated using Student's t-test or Wilcoxon U test. The significance level was set at 5%. RESULTS: The initial sample consisted of 49 patients aged 59±9 years with a body mass index of 29.8±5.1 kg/m 2 , who have had diabetes for a median time of 36 months (interquartile range of 1-240) and have been on metformin for a median time of 36 months (interquartile range of 1-180). Twenty-five patients left the study between the second and fourth meetings. Malondialdehyde levels differed between before and after pharmacotherapeutic follow-up, being positively correlated with blood glucose, glycohemoglobin, and triglyceride level, and negatively correlated with metformin and superoxide dismutase. Blood glucose, glycohemoglobin, and malondialdehyde levels increased, whereas metformin levels decreased in the group with diabetic complications, and there was a correlation between malondialdehyde and the number of diabetic complications per patient. CONCLUSIONS: In this sample of patients with type 2 diabetes mellitus treated with metformin, oxidative stress was more pronounced in those with poor glycemic control and diabetic complications.KEY WORDS: diabetes mellitus; oxidative stress; superoxide dismutase; malondialdehyde; metformin; diabetic complications. RESUMOOBJETIVOS: Avaliar parâmetros de estresse oxidativo em pacientes com diabetes mellitus tipo 2 em uso de metformina, relacionando estes valores a seus efeitos adversos, níveis plasmáticos, controle glicêmico, complicações diabéticas, perfil lipídico, e a influência do acompanhamento farmacoterapêutico. MÉTODOS: Foram avaliados pacientes com diabetes mellitus tipo 2, em uso de metformina, em acompanhamento farmacoterapêutico por quatro meses. O acompanhamento farmacoterapêutico consistiu na prestação de informações e no esclarecimento de dúvidas dos pacientes sobre a medicação e a doença. Além disto, foram verificados os horá...

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“…Plasma MDA was measured using the technique described by Antunes et al (2008), using high-performance liquid chromatography (HPLC). The chromatographer was composed of a system of pumps (LC-10AT), controller (SCL-10A), degasser (DGV-14A), column oven (CTO-10AS), autosampler (SIL-10AF), and a diode array detector (DAD) (SPD-M10A).…”

Section: Malondialdehyde (Mda)mentioning

confidence: 99%

A multibiomarker approach in rats to assess the impact of pollution on Sinos River, Southern Brazil

et al. 2010

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The aim of this study was to determine the feasibility of combining water quality analysis with different biomarkers to characterise the relationship between anthropogenic contamination and biotic response in the Sinos River, southern Brazil. Wistar rats were studied using three biomarkers combined with physical, chemical and microbiological analysis to assess the effects of pollution at four sampling sites. The induction of oxidative stress was quantified by MDA levels in peripheral blood, lymphocyte DNA damage was determined using the comet assay, and histopathological changes were analysed in the liver. After sampling, animals were allowed to drink the river water during a 48 hours period. No increase in oxidative stress and DNA damage was observed. However, liver damage was observed in the animals exposed to water samples, indicating that the Sinos River is contaminated with hepatotoxic substances. Water analyses confirmed that water quality decreased downriver.Keywords: genotoxicity, pollution, biomarkers, oxidative stress, tissue damage. Análise de múltiplos biomarcadores em ratos para determinar o impacto da poluição do rio dos SinosResumo O objetivo deste estudo foi determinar a viabilidade de combinar a análise da qualidade da água com o estudo de biomarcadores para caracterizar a relação entre contaminação antropogênica e resposta biótica no rio dos Sinos, sul do Brasil. Ratos Wistar machos foram estudados usando três biomarcadores combinados com análises físicas, químicas e microbiológicas para determinar os efeitos da poluição em quatro locais de amostragem de água. A indução do estresse oxidativo foi quantificada pelos níveis de MDA no sangue periférico, o dano do DNA linfocitário foi determinado usando o ensaio cometa, e as alterações histopatológicas foram analisadas no fígado. As amostras da água do rio dos Sinos (dos quatro pontos selecionados) foram administradas por via oral (como água para beber) por 48 horas. Nenhum aumento do estresse oxidativo ou do dano do DNA foi observado. Entretanto, dano do tecido hepático foi verificado nos animais expostos à ingestão das amostras da água, indicando que o rio dos Sinos está contaminado com substâncias hepatotóxicas. As análises das amostras de água confirmaram a redução da qualidade da água em direção à foz do rio.Palavras-chave: genotoxicidade, poluição, biomarcadores, estresse oxidativo, dano tecidual.

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Estudo pré-analítico e de validação para determinação de malondialdeído em plasma humano por cromatografia líquida de alta eficiência, após derivatização com 2,4-dinitrofenilhidrazina

Cited by 30 publications

References 20 publications

Evaluation of the anti-inflammatory and antioxidant effects of the sucralfate in diversion colitis

Evaluation of the anti-inflammatory and antioxidant effects of the sucralfate in diversion colitis

Oxidative stress in patients with type 2 diabetes mellitus treated with metformin

A multibiomarker approach in rats to assess the impact of pollution on Sinos River, Southern Brazil

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