Rapid prototyping of nano- and micro-patterned substrates for the control of cell neuritogenesis by topographic and chemical cues

Adv Healthcare Materials

Sitti

2016

A surface patterning technique and a specific and strong biotin-streptavidin bonding of bacteria on patterned surfaces are proposed to fabricate Janus particles that are propelled by the attached bacteria. Bacteria-driven Janus microswimmers with diameters larger than 3 μm show enhanced mean propulsion speed. Such microswimmers could be used for future applications such as targeted drug delivery and environmental remediation.

Section: Methodsmentioning

confidence: 99%

Patterned and Specific Attachment of Bacteria on Biohybrid Bacteria‐Driven Microswimmers

Adv Healthcare Materials

Sitti

2016

“…In the last few years, methods for the fabrication of nanoscale controlled topographies have been developed utilizing lithographic and etching techniques derived from the silicon microelectronics industry and can now be used to tailor surface chemistry and surface topography to elucidate how cells respond to nanotopography. This has immensely augmented the process to tailor the surface chemistry and surface topography to elucidate the cellular behavior—importantly, cell adhesion (including bacterial), activation, and differentiation fate . Micro‐ and nano‐engineered tools and techniques have played a critical role in discovering binding interactions between the cells and their supporting environment to explore how cells respond to nanostructures, but more work is needed to comprehend the mechanisms underlying cell responses.…”

Section: Current Methods To Study Cell–surface Interactions: Nanostrumentioning

confidence: 99%

Micro‐nanopatterning as tool to study the role of physicochemical properties on cell–surface interactions

J Biomedical Materials Res

Patil

Thombre

et al. 2013

The current nano-biotechnologies interfacing synthetic materials and cell biology requires a better understanding of cell-surface interactions on the micro-to-nanometer scale. Cell-substrate interactions are mediated by the presence of proteins adsorbed from biological fluids to the substrate. The effect of nanotopography and surface chemistry on protein adsorption as well as the mediation effect on subsequent cellular communication with substratum is not well documented. This review discusses the role of physicochemical properties of cell-surface interactions and state-of-the-art methods currently available for micro-nanoscale surface fabrication and patterning. We also briefly discuss the current surface patterning techniques that allow the combination of a fine and independent control on nanotopography and chemistry to understand the effect of surface nanoscale substrate morphology on cell-surface interactions which has never been realized in previous reports. In addition, we discuss the influence of various chemical patterning and modulation of the nano-topography of surfaces on cell functionality and phenotype.

“…Indeed, as soon as a biomaterial surface comes into contact with blood, serum or any other biological fluid, it is immediately covered by proteins that form an adsorbed layer. In other words, the interaction between a material and the biological environment is mediated by the protein adsorption process . More relevant literature explaining the role of nanostructured surfaces in proteins and biointerfacial interactions which are beyond the scope of this review can be found elsewhere …”

Section: Supersonic Cluster Beam Depositionmentioning

confidence: 99%

“…The recent application of a high‐throughput characterization approach to study the effect of surface nanoscale morphology on protein adsorption shed more light on this phenomenon. PSIM has been applied to ns‐TiO x for carrying out systematic characterization of the adsorption of proteins on nanostructured surfaces . With PSIM, it was possible to study the adsorption of a panel of three proteins (BSA, fibrinogen, and streptavidin) on a ns‐TiO x library composed by five families of samples each with different surface morphology [Fig.…”

Section: Supersonic Cluster Beam Depositionmentioning

confidence: 99%

Biotechnological applications of supersonic cluster beam‐deposited nanostructured thin films: Bottom‐up engineering to optimize cell–protein–surface interactions

J Biomedical Materials Res

2013

Technological innovations in biomaterial sciences harness nanoparticle (NP) production, manipulation, and deposition with supreme precision, enabling the development of industrial processes. This review first discusses the basic components of this approach, introducing cluster sources, experimental apparatus, and growth mechanisms for NP formation. The second part of this review provides an overview of how the nanoscale bottom-up engineering can control protein adsorption, which in turn determines the fate of nanostructured coating for prokaryotic and mammalian (primary and stem) cell interactions. In addition, we briefly address the implications of the cluster beam deposition technique for nanostructuration of biocompatible microdevices and its potential as a facile coating method to promote protein-surface interactions for microarray applications in biotechnology.