Rapid propagation of agave by in vitro tissue culture

Bình, Lê Trần; Muoi, Le Thi; Oanh, H. T. K.; Thang, Tran D.; Phòng, Đinh Thị

doi:10.1007/bf00116091

Cited by 40 publications

(34 citation statements)

References 8 publications

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“…These hormones have been used for the promotion of growth in several plants that form stolons [18,19]. We studied 64 culture conditions with various combinations of kinetin (0, 0.1, 0.5, 2.5 mg/L), GA3 (0, 0.01, 0.1, 1 mg/L), and NAA (0, 0.01, 0.1, 1 mg/L) for 4 weeks and measured the number of newly formed ramets and the weights of After growing aseptic plants on solid medium for more than one month, the plants were transferred to liquid medium in a plastic container.…”

Section: Analysis Of Effective Growth Conditions For Individual Youngmentioning

confidence: 99%

Establishment of an Aseptic Culture System and Analysis of the Effective Growth Conditions for Eleocharis acicularis Ramets for Use in Phytoremediation

et al. 2017

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Eleocharis acicularis, an aquatic macrophyte of the Cyperaceae family, has been shown to accumulate multiple heavy metals and has great potential for use in the phytoremediation of contaminated soil and water. To investigate the mechanism of accumulation of heavy metals in E. acicularis while excluding biotic and abiotic environmental effects and to acquire homogenous and sufficient populations of E. acicularis, we established an aseptic culture system and analyzed the applicability of this species for phytoremediation. Young ramet bases and stolon tips of E. acicularis grown in the field were sterilized, cultured, and established in an aseptic culture system, and the effective growth conditions of isolated ramets were determined. Isolated ramets grew remarkably well in a medium of pH 4.8 to 5.7 with 0.25 mg/L kinetin as a plant hormone. Furthermore, capacity for the accumulation of heavy metals was examined using E. acicularis subcultured with or without Si. Aseptically cultured E. acicularis showed a sufficient capacity for Cs and Zn accumulation and exceeded the criteria for hyperaccumulator plants in accumulating Pb, Cd, and In regardless of the addition or not of Si during its subculture. The aseptic culture of E. acicularis enhances its capacity for the accumulation of heavy metals and its applicability for phytoremediation.

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Section: Analysis Of Effective Growth Conditions For Individual Youngmentioning

confidence: 99%

Establishment of an Aseptic Culture System and Analysis of the Effective Growth Conditions for Eleocharis acicularis Ramets for Use in Phytoremediation

et al. 2017

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“…A straw was placed over the ovary to prevent oviposition by moths. Ovules were removed from the ovary as described in the tetrazolium experiment, and were placed on media made from a modified recipe of Murashige basal media agar (Murashige 1974;Binh et al 1990). The modified recipe contained 8% sucrose, 4.3 g Murashige and Skoog salts, 0.4 mg thiamine, 10 mg inositol, 2.0 mg IAA, and 1% agar per liter of media, and the pH was adjusted to 6.0.…”

Section: Effect Of Eggs On Ovules In Culturementioning

confidence: 99%

Effect of pollinator-inflicted ovule damage on floral abscission in the yucca-yucca moth mutualism: the role of mechanical and chemical factors

Marr

Pellmyr

2003

Oecologia

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The long-term persistence of obligate mutualisms (over 40 Mya in both fig/fig wasps and yucca/yucca moths) raises the question of how one species limits exploitation by the other species, even though there is selection pressure on individuals to maximize fitness. In the case of yuccas, moths serve as the plant's only pollinator, but eggs laid by the moths before pollination hatch into larvae that consume seeds. Previous studies have shown that flowers with high egg loads are more likely to abscise. This suggests that yucca flowers can select against moths that lay many eggs per flower through selective abscission of flowers; however, it is not known how yucca moths trigger floral abscission. We tested how the moth Tegeticula yuccasella triggers floral abscission during oviposition in Yucca filamentosa by examining the effects of ovipositor insertion and egg laying on ovule viability and floral abscission. Eggs are not laid at the site of ovipositor insertion: we used this separation to test whether wounded ovules were more closely associated with the ovipositor site or an egg's location. Using a tetrazolium stain to detect injured ovules, we determined whether the number of ovipositions affected the number of wounded ovules in naturally pollinated flowers. Two wounding experiments were used to test the effect of mechanical damage on the probability of floral abscission. The types of wounds in these experiments mimicked two types of oviposition-superficial oviposition in the ovary wall and oviposition into the locular cavity-that have been observed in species of Tegeticula. The effect of moth eggs on ovule viability was experimentally tested by culturing ovules in vitro, placing moth eggs on the ovules, and measuring changes in ovule viability with a tetrazolium stain. We found that ovules were physically wounded during natural oviposition. Ovules showed a visible wounding response in moth-pollinated flowers collected 7-12 h after oviposition. Exact location of wounded ovules relative to eggs and oviposition scars, as well as results from the artificial wounding experiments, showed that the moth ovipositor inflicts mechanical damage on the ovules. Significantly higher abscission rates were observed in artificially wounded flowers in which only 4-8% of the ovules were injured. Eggs did not affect ovule viability as measured by the tetrazolium stain. These results suggest that physical damage to ovules caused by ovipositing is sufficient to explain selective fruit abscission. Whether injury as a mechanism of selective abscission in yuccas is novel or a preadaptation will require further study.

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“…Roxb. ex Salm-Dyck (Binh et al 1990), A. parrasana A. Berger (Santacruz-Ruvalcaba et al 1999, A. victoria-reginae T. Moore (Rodríguez-Garay et al 1996, Martínez-Palacios et al 2003, A. sisalana Perrine ex Engelm. (Nikam 1997, Hazra et al 2002, Nikam et al 2003 and A. tequilana F.A.C.…”

Section: Introductionmentioning

confidence: 99%

Plant growth regulators optimization for maximize shoots number in Agave americana L. by indirect organogenesis

Reyes-Zambrano¹,

Lecona-Guzmán²,

Barredo-Pool³

et al. 2016

Gayana Bot.

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Rapid propagation of agave by in vitro tissue culture

Cited by 40 publications

References 8 publications

Establishment of an Aseptic Culture System and Analysis of the Effective Growth Conditions for Eleocharis acicularis Ramets for Use in Phytoremediation

Establishment of an Aseptic Culture System and Analysis of the Effective Growth Conditions for Eleocharis acicularis Ramets for Use in Phytoremediation

Effect of pollinator-inflicted ovule damage on floral abscission in the yucca-yucca moth mutualism: the role of mechanical and chemical factors

Plant growth regulators optimization for maximize shoots number in Agave americana L. by indirect organogenesis

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