Plant growth regulators optimization for maximize shoots number in Agave americana L. by indirect organogenesis

Reyes-Zambrano, Sheila Jazmín; Lecona-Guzmán, Carlos Alberto; Barredo-Pool, Felipe; Calderón, José Dolores Ambrosio; Abud‐Archila, Miguel; Rincón-Rosales, Reiner; Gutiérrez-Miceli, Federico Antonio

doi:10.4067/s0717-66432016000100014

Cited by 13 publications

(4 citation statements)

References 18 publications

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“…Due to their cell division capacity, they are considered a good source of explants. Our results are in the range reported in other works of A. Americana, regarding the percentage of callus where the meristem was used [20,26], finding percentages ranging from 80% to 100%. However, the callus obtained in those works was of the organogenic type, which is not desirable to generate a cell suspension.…”

Section: Discussionsupporting

confidence: 87%

Effect of Water Stress Induced by Polyethylene Glycol on Growth, Proline Accumulation in Agave americana L.

Enrique¹,

Alberto²,

Antonio³

et al. 2023

Phyton

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The effect of water deficit was determined on both in vitro and soil seedling as well as in cells in suspension of Agave americana L. In order to do the establishment of cells, the formation of callus was induced; for it two auxins were evaluated: 2,4-dichlorophenoxyacetic acid (2,4-D) and 4-mino-3,5,6-trichloropicolinic acid (picloram) at three concentrations (0.25, 0.5 and 0.75 mg L −1 ) in three explants (leaf, root and meristems) cultured in MS semisolid medium. The callogenesis response was related to the type and section of the explant, as well as the regulator used, and a cell suspension was established using 0.5 mg L −1 naphthaleneacetic acid (NAA) + 0.5 mg L −1 Benzylaminopurine (BAP). Seedlings were exposed to polyethyleneglycol (15% and 30% w/v) with a water potential of −0.87 and −2.67 MPa, respectively, under soil conditions. Water stress was applied through restricted irrigation. Fresh weight, root system growth, and chlorophyll concentration were some of the parameters that were affected by the effect of water deficit on A. americana L. Chlorophyll concentration values were significantly decreased by 15 at 30% PEG (19.6 SPAD units) compared to the control treatment. In in vitro plants, the highest concentration of proline was found in the roots, being the treatment with 30% polyethylene glycol where the highest concentration of this osmoregulator was obtained (62.5 mg g −1 DW). Under restricted irrigation conditions, an increase in proline concentration was observed both in the aerial part (2.2 µg 100 g −1 DW) and in the root system (1.8 µg 100 g −1 DW). However, the concentrations found were approximately ten times greater, less than those found under in vitro conditions. Therefore, the accumulation of proline can be considered an indicator of stress in Agave Americana L. growth in vitro.

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Section: Discussionsupporting

confidence: 87%

Effect of Water Stress Induced by Polyethylene Glycol on Growth, Proline Accumulation in Agave americana L.

Enrique¹,

Alberto²,

Antonio³

et al. 2023

Phyton

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“…Rooting and acclimatization of plantlets. Plantlets were rooting and acclimatization according to Reyes-Zambrano et al (2016). Two hundred and fifty plantlets with a welldeveloped root system were washed in running water to remove phytagel and transferred to unicel pots (12 • 10 cm) (one plantlet per unicel pot) containing peatmoss and agrolite mixture (1:1) for acclimatization.…”

Section: Methodsmentioning

confidence: 99%

In Vitro Propagation of Agave americana by Indirect Organogenesis

Lecona-Guzmán

Reyes-Zambrano

Barredo-Pool

et al. 2017

horts

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Factors such as slow growth, low rates of sexual and asexual reproduction, and viability of seeds among others limit the massive propagation of Agave americana L. by conventional methods. In this study, callus induction and shoot proliferation was determined in A. americana using Murashige and Skoog (MS) medium supplemented with dicholorophenoxyacetic acid (2,4-D) and 6-benzyl adenine (BA). Meristematic tissue was used as the explants, and were placed on MS medium supplemented with 30.0 g·L−1 sucrose with 0.11, 0.18, or 0.45 μm 2,4-D and 11.0, 22.0, 38.2, 44.0, 58.7, or 73.3 μm BA. Treatments were implemented according to factorial experimental design 3 × 6. After 1 month, the number of explants with callus was determined, whereas the numbers of shoots per explant were monitored after 4, 16, 20, and 36 weeks. The maximum percent of explants with callus was obtained with 0.11 μm 2,4-D and 58.7 and 73.3 μm BA, whereas the maximum numbers of shoots per explant (71) were obtained with 0.11 μm 2,4-D and 73.3 μm BA. The effect of different concentrations of indolebutyric acid (IBA) in the rooting of shoots was evaluated. There were no significant effects of IBA on the number of roots, root length, and axillary roots. Plantlets were acclimatized in the glasshouse and they did not show any phenotypic alteration. This is a highly efficient protocol for the in vitro propagation of A. americana via indirect organogenesis.

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“…In order to obtain the shoot apical meristem, the leaves, stems and roots were removed, explants were placed on MS (Murashige & Skoog) medium [19] supplemented with vitamins, sucrose (30 g/l), myo-inositol (0.228 mM), sodium phosphate (0.362 mM), phytagel (2.5 g/l), supplemented with 2,4-D (0.11 μM) and BAP (44 μM). Explants were incubated at 22°C ± 2 in continuous light [20]. The response was evaluated at eight weeks post culture and the percentage of callus induction was determined in each explant, and calculated with the following formula [21].…”

Section: Callus Induction and Proliferationmentioning

confidence: 99%

Morphometric and Biochemical Changes in Agave americana L. Plantlets Induced By Ethyl Methanesulfonate

Reyes-Zambrano

Ramírez-Merchant

Arias-Castro³

et al. 2019

Phyton

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A. americana L. is a crop with very little genetic variability. In order to evaluate the effect of ethyl methanesulfonate (EMS) to induce variability in in vitro plantlets of A. americana, different explants (meristems, leaves and roots) were evaluated for the production of callus. MS medium supplemented with ANA (2.68 μM) and BAP (2.68 μM) was used. Callus obtained from apical meristem were treated with 15 mM EMS for two hours after which shoot formation was induced using 2,4-D (0.11 μM) and BAP (44 μM). The EMS induced variations in the morphometric and morphological parameters of the plantlets obtained, with 60% of the plantlets presenting differences such as dwarfism and different leaf forms, without the presence of spines, as well as an increase in fructan content of 30% with respect to the control plantlets. PAL was increased and this activity is related with higher anthocyanins concentration in A. americana L. plantlets.

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Plant growth regulators optimization for maximize shoots number in Agave americana L. by indirect organogenesis

Cited by 13 publications

References 18 publications

Effect of Water Stress Induced by Polyethylene Glycol on Growth, Proline Accumulation in Agave americana L.

Effect of Water Stress Induced by Polyethylene Glycol on Growth, Proline Accumulation in Agave americana L.

In Vitro Propagation of Agave americana by Indirect Organogenesis

Morphometric and Biochemical Changes in Agave americana L. Plantlets Induced By Ethyl Methanesulfonate

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